BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,533	C→T	A99V (GCG→GTG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,533	0	C	T	98.9%	657.0 / ‑4.8	188	A99V (GCG→GTG)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (92/94); total (93/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 195 total aligned reads displayed.

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGT  >  NC_000913/8443‑8621

agATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgtgc                                                                                        <  2:3361773/93‑1 (MQ=255)

  aTCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGt                                                                                      >  2:548192/1‑93 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                     >  1:4178913/1‑93 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                     <  1:4376906/93‑1 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                     >  1:4544755/1‑93 (MQ=255)

     gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCtt                                                                                   <  2:4298257/93‑1 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                 >  2:4624173/1‑93 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                 <  1:2135280/93‑1 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                 <  2:2365673/93‑1 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                <  2:4244205/93‑1 (MQ=255)

         aCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCt                                                                               <  2:3144462/93‑1 (MQ=255)

         aCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCt                                                                               <  1:907191/93‑1 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                              <  2:4729749/93‑1 (MQ=255)

           gcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAt                                                                             <  1:4516040/93‑1 (MQ=255)

           gcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAt                                                                             >  1:4591710/1‑93 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                          >  1:233675/1‑93 (MQ=255)

               ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAGCTGAAGTTGATGTGCGTCTTTCCTATGaca                                                                         >  1:4576338/1‑93 (MQ=255)

               ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGaca                                                                         >  2:2951859/1‑93 (MQ=255)

               ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGaca                                                                         >  2:1818903/1‑93 (MQ=255)

                  acAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAACg                                                                      >  1:2450696/1‑93 (MQ=255)

                   cAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGa                                                                     >  1:838156/1‑93 (MQ=255)

                         ggCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTc                                                               >  2:75046/1‑93 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                             >  2:3426207/1‑93 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                             <  2:1665202/93‑1 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                             <  2:1665203/93‑1 (MQ=255)

                             gTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAtt                                                           <  2:1570300/93‑1 (MQ=255)

                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                          <  1:3031979/93‑1 (MQ=255)

                              aaaaTATTGCTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCATCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                          <  2:1089280/92‑1 (MQ=255)

                               aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                                         <  2:3203574/93‑1 (MQ=255)

                               aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                                         >  2:1528475/1‑93 (MQ=255)

                                aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCg                                                        >  1:1021405/1‑93 (MQ=255)

                                 atatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGa                                                       >  1:4507268/1‑93 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                      >  1:663051/1‑93 (MQ=255)

                                   atTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                     >  1:1428901/1‑93 (MQ=255)

                                   atTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                     <  1:3178870/93‑1 (MQ=255)

                                   atTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                     <  1:2347274/93‑1 (MQ=255)

                                    ttGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCACTTGCGAAAg                                                    >  2:491384/1‑93 (MQ=255)

                                    ttGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAg                                                    <  1:56507/93‑1 (MQ=255)

                                     tGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGc                                                   <  1:4343198/93‑1 (MQ=255)

                                     tGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGc                                                   <  1:3403283/93‑1 (MQ=255)

                                     tGGTCTGGAAAGCCTGAAACCGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGc                                                   <  1:4006320/93‑1 (MQ=255)

                                        tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaa                                                >  2:1172796/1‑93 (MQ=255)

                                         cTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                               <  2:4429748/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                              <  1:1241924/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                              >  2:284952/1‑93 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                              <  2:3820909/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                              >  1:4712121/1‑93 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                              <  2:1877156/93‑1 (MQ=255)

                                           ggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                                             <  1:2484764/93‑1 (MQ=255)

                                           ggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                                             >  2:199149/1‑93 (MQ=255)

                                             aaaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                                           <  2:2637338/93‑1 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                          >  2:675193/1‑93 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                          <  1:4489331/93‑1 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                          <  1:3963166/93‑1 (MQ=255)

                                               aTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTg                                         >  2:238153/1‑93 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                        >  1:1628448/1‑93 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                        >  1:1720993/1‑93 (MQ=255)

                                                  cTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                      >  1:1494594/1‑93 (MQ=255)

                                                   tGAAACTGGTTCTGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGTCTGATCa                                     >  1:2784191/1‑93 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                     <  1:270040/93‑1 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                     >  2:4328205/1‑93 (MQ=255)

                                                    gAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaa                                    >  1:1363411/1‑93 (MQ=255)

                                                     aaaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaaa                                   <  2:1270669/93‑1 (MQ=255)

                                                     aaaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaaa                                   <  2:871303/93‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                  >  1:1392648/1‑93 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAct                                 >  2:715541/1‑93 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAACACGCCTGATCAAAct                                 >  2:4040983/1‑93 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCGCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAct                                 <  1:2720812/93‑1 (MQ=255)

                                                        cTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActc                                <  2:2435243/93‑1 (MQ=255)

                                                          ggTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa                              >  2:113253/1‑93 (MQ=255)

                                                          ggTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa                              <  1:2942707/93‑1 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                             >  1:3343283/1‑93 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                             <  1:1996469/93‑1 (MQ=255)

                                                            ttCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACa                            >  2:1701938/1‑93 (MQ=255)

                                                              ccGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAc                          <  1:3590852/93‑1 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                        >  2:1307554/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                       >  2:2879588/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                       >  1:1592512/1‑93 (MQ=255)

                                                                   ccGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGc                     <  1:1745081/93‑1 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGCAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                    >  1:2253051/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                   <  2:4157522/93‑1 (MQ=255)

                                                                      tATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAAAACGAGCCTgg                  >  1:975609/1‑93 (MQ=255)

                                                                       aTCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt                 >  1:2706469/1‑93 (MQ=255)

                                                                       aTCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt                 <  2:1355404/93‑1 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                <  1:206094/93‑1 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                <  2:587511/93‑1 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt               <  2:4697439/93‑1 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt               >  2:3550169/1‑93 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCC‑GATCAAACTCTAAAACGATGCCGGTAtt              >  2:2055433/1‑93 (MQ=255)

                                                                           cAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTa             <  1:1164465/93‑1 (MQ=255)

                                                                             acggAAGTTGAGGTGCGGCGTGCCGATGACACCGAAGCGGCAATGGCGAAAGCAAAACGCCTGACCAAACTCGACAACGAGGCGGGGAGTAGc           >  2:3617521/4‑93 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc           >  1:3693599/1‑93 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc           <  1:2622868/93‑1 (MQ=255)

                                                                               tGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCaa         <  1:3761159/93‑1 (MQ=255)

                                                                                 aaGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACg       >  1:479974/1‑93 (MQ=255)

                                                                                  aGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGa      <  2:2494173/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt     >  2:2274276/1‑93 (MQ=255)

                                                                                    ttGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc    <  1:3917542/93‑1 (MQ=255)

                                                                                    ttGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc    >  2:944100/1‑93 (MQ=255)

                                                                                     tGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCg   >  1:1846417/1‑93 (MQ=255)

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGT  >  NC_000913/8443‑8621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`