BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,388	A→G	S298P (TCC→CCC)	b1337 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,388	0	A	G	97.6%	396.3 / ‑0.5	127	S298P (TCC→CCC)	b1337	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (57/67); total (58/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 128 total aligned reads displayed.

CAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400299‑1400477

cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        <  1:3075323/93‑1 (MQ=255)

cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        >  1:2415035/1‑93 (MQ=255)

cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        >  2:4184628/1‑93 (MQ=255)

cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        >  2:4532070/1‑93 (MQ=255)

  gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      <  1:2581354/93‑1 (MQ=255)

  gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      <  2:1290036/93‑1 (MQ=255)

  gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      >  1:4098423/1‑93 (MQ=255)

    tcttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCt                                                                                    <  1:2598512/93‑1 (MQ=255)

    tcttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCt                                                                                    <  1:2276834/93‑1 (MQ=255)

     cttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   >  1:4563222/1‑93 (MQ=255)

     cttcGGAGTTCCATGCCGTGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   <  1:1942057/93‑1 (MQ=255)

      ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  1:3990085/1‑93 (MQ=255)

      ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  1:2469417/1‑93 (MQ=255)

      ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  <  2:4176929/93‑1 (MQ=255)

      ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  <  2:2694528/93‑1 (MQ=255)

       tcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTg                                                                                 >  2:4619343/1‑93 (MQ=255)

       tcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTg                                                                                 <  1:470333/93‑1 (MQ=255)

        cGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGTCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGt                                                                                <  2:2735777/93‑1 (MQ=255)

        cGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGt                                                                                >  2:557556/1‑93 (MQ=255)

         ggAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTc                                                                               >  1:1366116/1‑93 (MQ=255)

          gAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCg                                                                              <  1:3971/93‑1 (MQ=255)

           aGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGa                                                                             <  1:3723238/93‑1 (MQ=255)

            gTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGaa                                                                            >  2:1198167/1‑93 (MQ=255)

            gTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGaa                                                                            <  1:4743538/93‑1 (MQ=255)

             ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAg                                                                           >  1:3611316/1‑93 (MQ=255)

             ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAg                                                                           <  2:2142293/93‑1 (MQ=255)

             ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAg                                                                           <  1:1215447/93‑1 (MQ=255)

              tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          <  2:1458565/93‑1 (MQ=255)

              tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          <  2:283376/93‑1 (MQ=255)

              tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          <  1:1026929/93‑1 (MQ=255)

               ccATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCg                                                                         >  2:2458239/1‑93 (MQ=255)

                cATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCgg                                                                        >  1:1880366/1‑93 (MQ=255)

                 aTTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGc                                                                       >  1:525065/1‑93 (MQ=255)

                  ttCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCa                                                                      >  2:2350886/1‑93 (MQ=255)

                  ttCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCa                                                                      >  1:813095/1‑93 (MQ=255)

                   tCCGGGGTACCAAAATGGGATNGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     <  1:3893490/93‑1 (MQ=255)

                   tCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     <  2:2293596/93‑1 (MQ=255)

                   tCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     <  2:2933154/93‑1 (MQ=255)

                   tCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     >  1:2511230/1‑93 (MQ=255)

                      ggggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCa                                                                  >  2:381709/1‑93 (MQ=255)

                      ggggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCa                                                                  >  2:4027662/1‑93 (MQ=255)

                       gggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCaa                                                                 >  2:3798695/1‑93 (MQ=255)

                        ggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAAc                                                                >  1:3566249/1‑93 (MQ=255)

                           aCCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGt                                                             <  1:4082665/93‑1 (MQ=255)

                           aCCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGt                                                             >  2:4529834/1‑93 (MQ=255)

                           aCCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGt                                                             <  1:3977099/93‑1 (MQ=255)

                             cAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTgg                                                           <  2:1226904/93‑1 (MQ=255)

                              aaaaTGGGATAGGGTTTGGTACATGGCCTTTTGTGGGGCGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          <  2:3523386/93‑1 (MQ=255)

                              aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          <  2:3523385/93‑1 (MQ=255)

                              aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          >  1:880801/1‑93 (MQ=255)

                               aaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGtt                                                         <  1:143412/93‑1 (MQ=255)

                                 aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       >  2:3204204/1‑93 (MQ=255)

                                 aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       >  2:392368/1‑93 (MQ=255)

                                 aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       >  2:4121458/1‑93 (MQ=255)

                                  tGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCg                                                      >  2:340201/1‑93 (MQ=255)

                                  tGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCg                                                      >  1:2095010/1‑93 (MQ=255)

                                   tggATAGGGCCTAGTACATGTCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCgg                                                     <  1:4673142/92‑1 (MQ=255)

                                    ggATAGGGCCGGGTACATGGCATTTTCTAAGTGGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGt                                                    <  1:1318927/93‑1 (MQ=255)

                                      aTAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtca                                                  >  1:3001038/1‑93 (MQ=255)

                                        aGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatc                                                <  2:428043/93‑1 (MQ=255)

                                        aGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatc                                                <  2:428042/93‑1 (MQ=255)

                                         gggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatca                                               >  2:4163009/1‑93 (MQ=255)

                                          ggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              <  1:4023271/93‑1 (MQ=255)

                                              tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          <  1:934785/93‑1 (MQ=255)

                                               ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCg                                         >  2:4468471/1‑93 (MQ=255)

                                                gTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGc                                        <  2:750330/93‑1 (MQ=255)

                                                 tACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCa                                       >  1:2541927/1‑93 (MQ=255)

                                                  aCATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAc                                      >  2:2536857/1‑93 (MQ=255)

                                                  aCATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAc                                      >  1:2346357/1‑93 (MQ=255)

                                                   cATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAcc                                     >  2:3277000/1‑93 (MQ=255)

                                                   cATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAcc                                     <  1:2284841/93‑1 (MQ=255)

                                                    aTGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    >  1:2114052/1‑93 (MQ=255)

                                                     tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCtt                                   <  2:4041918/93‑1 (MQ=255)

                                                      ggCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTc                                  <  2:3179390/93‑1 (MQ=255)

                                                        cATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCgg                                <  2:2042839/93‑1 (MQ=255)

                                                        cATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCgg                                >  1:3706519/1‑93 (MQ=255)

                                                         aTTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGc                               <  2:1333875/93‑1 (MQ=255)

                                                         aTTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGc                               <  2:1665734/93‑1 (MQ=255)

                                                          ttttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCg                              >  1:2525058/1‑93 (MQ=255)

                                                            ttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAt                            <  2:1048852/93‑1 (MQ=255)

                                                            ttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAt                            <  1:1705181/93‑1 (MQ=255)

                                                              cTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAttt                          <  1:1584945/93‑1 (MQ=255)

                                                               tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         <  1:3402523/93‑1 (MQ=255)

                                                                 aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       >  1:1329655/1‑93 (MQ=255)

                                                                 aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       <  2:2318875/93‑1 (MQ=255)

                                                                  ggTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGc                      >  2:2511853/1‑93 (MQ=255)

                                                                  ggTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGc                      <  1:1428926/93‑1 (MQ=255)

                                                                   gTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCg                     <  1:522905/93‑1 (MQ=255)

                                                                     gCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGAc                   <  1:2770148/93‑1 (MQ=255)

                                                                       gATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGAccc                 >  1:3535851/1‑93 (MQ=255)

                                                                         ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               >  2:3621182/1‑93 (MQ=255)

                                                                          tCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAt              <  2:536760/93‑1 (MQ=255)

                                                                           cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             >  2:4540545/1‑93 (MQ=255)

                                                                                  aaaCTGGGACAGGCTTTGTCGAAGCTGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  1:4026692/92‑1 (MQ=255)

                                                                                   aaCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAatat     <  1:2831352/93‑1 (MQ=255)

                                                                                   aaCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAatat     <  2:3874042/93‑1 (MQ=255)

                                                                                   aaCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAatat     <  1:1477661/93‑1 (MQ=255)

                                                                                     cTGGGACAGGCTTTGTCGAATCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgc   >  2:3469440/1‑93 (MQ=255)

                                                                                      tGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgct  >  1:960042/1‑93 (MQ=255)

                                                                                      tGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgct  <  1:1060508/93‑1 (MQ=255)

CAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400299‑1400477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`