BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,658	C→T	P172P (CCC→CCT)	ydaU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,658	0	C	T	97.8%	448.7 / ‑1.9	135	P172P (CCC→CCT)	ydaU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (70/62); total (73/62)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 143 total aligned reads displayed.

TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747

  ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGCTACAGATAAAGATCTAAAAACAGATcctcccc                                                                                         <  2:3943439/93‑1 (MQ=255)

  ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                         <  1:2034502/93‑1 (MQ=255)

   tCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCt                                                                                        <  2:3682920/93‑1 (MQ=255)

   tCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCt                                                                                        <  2:1268483/93‑1 (MQ=255)

   tCAAACAGATGTTGAAGTGGTGATTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCACACCt                                                                                        >  2:2385841/1‑93 (MQ=255)

    cAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTa                                                                                       >  1:741503/1‑93 (MQ=255)

     aaaCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTaa                                                                                      >  2:1611429/1‑93 (MQ=255)

      aaCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTaaa                                                                                     >  1:3002546/1‑93 (MQ=255)

      aaCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTaaa                                                                                     >  1:4076551/1‑93 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    >  2:1006787/1‑93 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    <  2:284195/93‑1 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    <  2:3343953/93‑1 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    >  1:4385450/1‑93 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    >  2:2148636/1‑93 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                    >  1:315858/1‑93 (MQ=255)

        cAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑c                                                                                 <  1:1397961/93‑1 (MQ=255)

        cAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑c                                                                                 <  1:2767568/93‑1 (MQ=255)

         aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑cc                                                                                >  1:3648290/1‑93 (MQ=255)

         aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑cc                                                                                <  1:3034423/93‑1 (MQ=255)

         aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑cc                                                                                <  2:1351697/93‑1 (MQ=255)

         aGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑cc                                                                                >  2:1245938/1‑93 (MQ=255)

           aTGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑cccc                                                                              <  1:4162353/93‑1 (MQ=255)

            tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑ccccc                                                                             >  1:2630793/1‑93 (MQ=255)

              ttGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑ccccccc                                                                           <  1:4199002/93‑1 (MQ=255)

              ttGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑ccccccc                                                                           <  2:4582367/93‑1 (MQ=255)

                gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCgg                                                                         <  1:2798996/93‑1 (MQ=255)

                 aaGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCggg                                                                        <  1:3086985/93‑1 (MQ=255)

                 aaGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCggg                                                                        <  2:4480312/93‑1 (MQ=255)

                  aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCgggg                                                                       <  1:4685478/93‑1 (MQ=255)

                   gtggtgTTTGACCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCggggg                                                                      <  2:4636246/93‑1 (MQ=255)

                   gtggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCggggg                                                                      >  2:3829996/1‑93 (MQ=255)

                     ggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGaa                                                                    >  1:3947728/1‑93 (MQ=255)

                         tttGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGa                                                                >  1:3344324/1‑93 (MQ=255)

                           tGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAgg                                                              <  2:1615041/93‑1 (MQ=255)

                            gAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGgt                                                             <  2:3776058/93‑1 (MQ=255)

                             aaCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGgtg                                                            >  1:336870/1‑93 (MQ=255)

                             aaCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGgtg                                                            <  1:971874/93‑1 (MQ=255)

                              aCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGgtgt                                                           <  1:1487733/93‑1 (MQ=255)

                               cATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTc                                                          <  1:2639177/93‑1 (MQ=255)

                                atgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCTAGGTGTCa                                                         >  1:3444641/1‑93 (MQ=255)

                                atgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCa                                                         >  1:3544059/1‑93 (MQ=255)

                                atgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCa                                                         >  2:2187061/1‑93 (MQ=255)

                                 tgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCATAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCaa                                                        >  1:1231294/1‑93 (MQ=255)

                                  gatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCaaa                                                       >  1:64649/1‑93 (MQ=255)

                                   atgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCaaaa                                                      <  1:676299/93‑1 (MQ=255)

                                      tCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGt                                                   <  1:649287/93‑1 (MQ=255)

                                      tCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGt                                                   >  1:3598670/1‑93 (MQ=255)

                                          cacaAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGa                                               >  2:373169/1‑93 (MQ=255)

                                            caAAGGCAATTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAcc                                             <  2:4110589/93‑1 (MQ=255)

                                            caAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCTCCCCGGGGGAATCGAGGTCTCAAAAAGATTGAcc                                             >  1:1489706/1‑93 (MQ=255)

                                            caAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAcc                                             >  2:1881292/1‑93 (MQ=255)

                                             aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAccc                                            >  1:4317852/1‑93 (MQ=255)

                                                ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCTCACCGGGGAAATCGAGGTGTCAAACAGTTTGAGCctct                                         >  1:4660250/1‑93 (MQ=255)

                                                ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         <  1:837562/93‑1 (MQ=255)

                                                ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         >  2:553744/1‑93 (MQ=255)

                                                ggCAACTAATAAAGATACAGATAAAGATCTAAAAACAGACCCTCCCCTAAGT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                         <  1:837561/93‑1 (MQ=255)

                                                   aaCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGa                                      >  2:3159041/1‑93 (MQ=255)

                                                   aaCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGa                                      >  2:3159040/1‑93 (MQ=255)

                                                    aCTAATAAAGATACAGATACAGATCTAAACACAGATCCCCCCCTAACT‑‑CCCCCCCGTGGGAATCGACGTGTCAAAAAGCTTGACCATCTGGat                                     >  1:2772293/1‑93 (MQ=255)

                                                    aCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGat                                     >  1:237222/1‑93 (MQ=255)

                                                      taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   <  1:3969520/93‑1 (MQ=255)

                                                       aataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATAtt                                  >  2:835750/1‑93 (MQ=255)

                                                       aataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATAtt                                  >  2:307626/1‑93 (MQ=255)

                                                       aataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATAtt                                  <  1:3212847/93‑1 (MQ=255)

                                                        ataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTa                                 >  1:2747287/1‑93 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  1:4635827/1‑93 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                <  1:877878/93‑1 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  2:124582/1‑93 (MQ=255)

                                                          aaaGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACt                               <  2:619692/93‑1 (MQ=255)

                                                           aaGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACtt                              <  2:1129381/93‑1 (MQ=255)

                                                            aGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACttt                             >  1:398033/1‑93 (MQ=255)

                                                             gATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTg                            >  2:329346/1‑93 (MQ=255)

                                                              aTACAGATAAAAATCTAAAAACAGATCCTCCCCTAAAC‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGc                           <  1:3492215/93‑1 (MQ=255)

                                                               tACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGcc                          <  1:2070402/93‑1 (MQ=255)

                                                                 cAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGa                        >  1:2799213/1‑93 (MQ=255)

                                                                  aGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGaa                       <  2:580549/93‑1 (MQ=255)

                                                                    aTAAAGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACt                     <  1:3867257/93‑1 (MQ=255)

                                                                       aaGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGa                  <  1:3003391/93‑1 (MQ=255)

                                                                        aGATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 >  2:2318888/1‑93 (MQ=255)

                                                                         gATCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAtt                >  1:2330377/1‑93 (MQ=255)

                                                                          aTCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAttt               >  2:2665265/1‑93 (MQ=255)

                                                                          aTCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAttt               >  2:2805613/1‑93 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              >  2:1453301/1‑93 (MQ=255)

                                                                              aaaaaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGt           >  2:791841/1‑93 (MQ=255)

                                                                                aaaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtct         >  2:1614435/1‑93 (MQ=255)

                                                                                aaaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtct         <  1:3549389/93‑1 (MQ=255)

                                                                                 aaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtctc        >  2:110108/1‑93 (MQ=255)

                                                                                 aaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtctc        <  2:3946087/93‑1 (MQ=255)

                                                                                 aaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtctc        >  1:1604424/1‑93 (MQ=255)

                                                                                 aaCAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtctc        >  2:1831757/1‑93 (MQ=255)

                                                                                   cAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGc      <  2:4291634/93‑1 (MQ=255)

                                                                                   cAGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGc      >  2:2110988/1‑93 (MQ=255)

                                                                                    aGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt     <  2:3630641/93‑1 (MQ=255)

                                                                                    aGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt     >  1:209897/1‑93 (MQ=255)

                                                                                    aGATCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt     >  2:1503467/1‑93 (MQ=255)

                                                                                      aTCCTCCCCTAAATCCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt     >  2:4501163/1‑93 (MQ=255)

                                                                                      aTCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCttt   <  2:2225619/93‑1 (MQ=255)

                                                                                      aTCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCttt   <  1:1830237/93‑1 (MQ=255)

                                                                                      aTCCTCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCttt   >  1:1352311/1‑93 (MQ=255)

                                                                                       tcctccCCTAAAT‑‑CCCCCCCGGGGGCATCGAGCCGTCAAAAAGCTTGCCTCTCTGGATATTACTTTGCCGTACTGGTTCTCTCTCTCGCTTTg  >  2:13686/1‑93 (MQ=255)

TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`