BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	126,160	A→G	T156A (ACC→GCC)	aceF →	pyruvate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	126,160	0	A	G	97.1%	411.6 / 1.1	136	T156A (ACC→GCC)	aceF	pyruvate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (63/69); total (65/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 143 total aligned reads displayed.

AAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCG  >  NC_000913/126070‑126249

 aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCg                                                                                        <  1:2648520/93‑1 (MQ=255)

  aGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGt                                                                                       <  1:2278181/93‑1 (MQ=255)

  aGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGt                                                                                       >  1:3374564/1‑93 (MQ=255)

   gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTg                                                                                      >  1:1015958/1‑93 (MQ=255)

   gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTg                                                                                      <  1:2837755/93‑1 (MQ=255)

     tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGaa                                                                                    >  1:3368694/1‑93 (MQ=255)

       gCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAag                                                                                  <  1:4308280/93‑1 (MQ=255)

        cGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAaga                                                                                 >  2:4531215/1‑93 (MQ=255)

         gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGGAagag                                                                                >  2:2558863/1‑93 (MQ=255)

         gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAagag                                                                                >  2:2558864/1‑93 (MQ=255)

          aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAgaga                                                                               <  2:1176176/93‑1 (MQ=255)

          aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAgaga                                                                               <  1:4745570/93‑1 (MQ=255)

          aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAgaga                                                                               >  2:287485/1‑93 (MQ=255)

           tAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGAt                                                                              <  2:1653793/93‑1 (MQ=255)

           tAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGAt                                                                              >  1:397425/1‑93 (MQ=255)

            aaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATc                                                                             <  2:1183661/93‑1 (MQ=255)

             aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCa                                                                            <  1:4075674/93‑1 (MQ=255)

             aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCa                                                                            <  1:4075673/93‑1 (MQ=255)

             aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCa                                                                            >  2:592489/1‑93 (MQ=255)

             aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCAACAAGGCTTCTATGGAAGGTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCa                                                                            >  1:4244101/1‑93 (MQ=255)

              aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCaa                                                                           >  2:972574/1‑93 (MQ=255)

               gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCaaa                                                                          <  1:1021227/93‑1 (MQ=255)

               gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCaaa                                                                          <  1:1037831/93‑1 (MQ=255)

                ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAg                                                                         <  2:1414596/93‑1 (MQ=255)

                   gaGCTGAACAGTCGCTGATCGCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGa                                                                      <  1:4659565/92‑1 (MQ=255)

                   aaGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGa                                                                      >  1:2819702/1‑93 (MQ=255)

                   aaGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGa                                                                      <  1:4629722/93‑1 (MQ=255)

                    aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGaa                                                                     <  1:4511112/93‑1 (MQ=255)

                     gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAAc                                                                    <  2:3186710/93‑1 (MQ=255)

                       tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGt                                                                  >  1:4404258/1‑93 (MQ=255)

                       tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGt                                                                  <  2:4503809/93‑1 (MQ=255)

                       tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGt                                                                  <  1:3404257/93‑1 (MQ=255)

                        gagcAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTGTGGAAGTTCGGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTg                                                                 <  1:2802633/90‑1 (MQ=21)

                         aaCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTgg                                                                <  2:1213244/93‑1 (MQ=255)

                          aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTggg                                                               >  2:4587531/1‑93 (MQ=255)

                          aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTggg                                                               <  2:2484616/93‑1 (MQ=255)

                             gTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTgc                                                            >  1:2635005/1‑92 (MQ=255)

                             gTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGa                                                            >  2:3676562/1‑93 (MQ=255)

                             gTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGa                                                            <  1:3650360/93‑1 (MQ=255)

                             gTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGa                                                            >  2:1280551/1‑93 (MQ=255)

                               cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                                          <  1:1552919/93‑1 (MQ=255)

                                gCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCTGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACaa                                                         >  1:4120578/1‑93 (MQ=255)

                                ccTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACaa                                                         <  1:1092176/92‑1 (MQ=255)

                                 cTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa                                                        >  2:4543866/1‑93 (MQ=255)

                                  tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg                                                       >  1:1111540/1‑93 (MQ=255)

                                   gATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt                                                      <  1:2265229/93‑1 (MQ=255)

                                   gATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt                                                      <  1:4046068/93‑1 (MQ=255)

                                     tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAGGAGCTCAAAGTGAACGTGGGCGACAAAgtgt                                                    >  2:1639413/1‑93 (MQ=17)

                                     tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtgt                                                    >  1:2800842/1‑93 (MQ=255)

                                     tCACCGTAGAAGGCCACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtgt                                                    <  2:1670541/93‑1 (MQ=255)

                                      cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                                                   <  1:4660869/93‑1 (MQ=255)

                                       aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCt                                                  <  2:2946899/93‑1 (MQ=255)

                                       aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCt                                                  <  2:3241219/93‑1 (MQ=255)

                                        ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa                                                 >  1:3426515/1‑93 (MQ=255)

                                        ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa                                                 >  2:903119/1‑93 (MQ=255)

                                         cGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc                                                <  2:2031512/93‑1 (MQ=255)

                                         cGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc                                                >  2:2048983/1‑93 (MQ=255)

                                         cGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc                                                >  1:805523/1‑93 (MQ=255)

                                         cGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc                                                <  1:1615921/93‑1 (MQ=255)

                                          gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAcc                                               <  2:1411114/93‑1 (MQ=255)

                                           tAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCg                                              >  1:1327462/1‑93 (MQ=255)

                                             gAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGc                                            >  2:4433584/1‑93 (MQ=255)

                                              aaGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTTACAAAGTGTCTACCGGCt                                           <  2:3866560/93‑1 (MQ=255)

                                               aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                                          <  2:1322658/93‑1 (MQ=255)

                                               aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                                          <  2:1295790/93‑1 (MQ=255)

                                                     cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAt                                    <  1:4642194/93‑1 (MQ=255)

                                                        ggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt                                 <  2:2400860/93‑1 (MQ=255)

                                                         gCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATg                                <  1:3031462/93‑1 (MQ=255)

                                                          cTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAttg                               >  2:1340913/1‑91 (MQ=255)

                                                          cTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg                               >  2:3665781/1‑93 (MQ=255)

                                                           ttCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt                              >  1:2718991/1‑93 (MQ=255)

                                                            tCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGACCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGCTTGTGGtg                             >  2:1414723/1‑92 (MQ=37)

                                                             cTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCt                            <  1:1121053/93‑1 (MQ=255)

                                                              tATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCtt                           <  2:2154753/93‑1 (MQ=255)

                                                              tATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGGCtt                           >  2:4081894/1‑93 (MQ=255)

                                                               aTGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTc                          <  2:3408481/93‑1 (MQ=255)

                                                                tGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCg                         >  1:2047125/1‑93 (MQ=255)

                                                                 ggAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATTGTCTTTGa                        >  2:3647594/1‑93 (MQ=255)

                                                                  gAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCgga                       >  1:2340751/1‑91 (MQ=255)

                                                                  gAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa                       >  1:2480113/1‑93 (MQ=255)

                                                                    aGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGt                     <  2:547517/93‑1 (MQ=255)

                                                                     gTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                    >  2:4378769/1‑93 (MQ=255)

                                                                     gTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                    <  1:3155955/93‑1 (MQ=255)

                                                                     gTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                    <  1:3077513/93‑1 (MQ=255)

                                                                     gTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                    <  1:300320/93‑1 (MQ=255)

                                                                     gTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                    <  1:1841279/93‑1 (MQ=255)

                                                                      ttCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcg                   >  2:2909381/1‑93 (MQ=255)

                                                                       tCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgc                  <  1:3947406/93‑1 (MQ=255)

                                                                       tCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgc                  <  1:2988113/93‑1 (MQ=255)

                                                                        ccGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTTGAAGTcgcg                 >  1:2081693/1‑93 (MQ=255)

                                                                        ccGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg                 <  1:3572356/93‑1 (MQ=255)

                                                                             tCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGGTTATGGTCTTTGAAAGCGCGGGGGa            >  1:1510736/1‑93 (MQ=255)

                                                                             tCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCCGATTATGGGCTTCGAAGGCGCgggggg            >  1:2198363/1‑90 (MQ=255)

                                                                              ccGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGaa           <  1:1815638/93‑1 (MQ=255)

                                                                               cGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAg          <  2:3966420/93‑1 (MQ=255)

                                                                                gTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGGGaaac         >  2:2187497/1‑91 (MQ=255)

                                                                                 tttGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCa        <  1:3375020/93‑1 (MQ=255)

                                                                                    gCTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgc     <  2:3426030/93‑1 (MQ=255)

                                                                                     cTGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgcg    <  1:1731560/93‑1 (MQ=255)

                                                                                      tGGCGCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgcgc   >  1:1776101/1‑93 (MQ=255)

AAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCG  >  NC_000913/126070‑126249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`