BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,461,082	C→T	P312S (CCA→TCA)	b1398 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,461,082	0	C	T	97.6%	434.5 / ‑2.0	127	P312S (CCA→TCA)	b1398	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (66/58); total (68/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 135 total aligned reads displayed.

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCC  >  NC_000913/1460992‑1461170

 gTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAg                                                                                       <  2:1885188/93‑1 (MQ=255)

  tGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAgg                                                                                      >  1:2993941/1‑93 (MQ=255)

  tGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAgg                                                                                      >  2:2086684/1‑93 (MQ=255)

   gAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGa                                                                                     <  2:2574449/93‑1 (MQ=255)

     aCATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAAc                                                                                   >  1:2081435/1‑93 (MQ=255)

      cATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACg                                                                                  <  1:627151/93‑1 (MQ=255)

      cATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACg                                                                                  >  1:484150/1‑93 (MQ=255)

       aTGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACgg                                                                                 >  1:3003795/1‑93 (MQ=255)

        tGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgc                                                                                <  1:155011/93‑1 (MQ=255)

           cGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGcgcg                                                                             >  1:2474254/1‑93 (MQ=255)

             aaCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCggc                                                                           >  2:397627/1‑92 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGAc                                                                          >  2:2104504/1‑93 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACtg                                                                        >  1:4513663/1‑92 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                       <  2:2925970/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                       >  2:2806688/1‑93 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                       >  1:2528840/1‑93 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGCCTGTTATCAGGAACGGCGCGCACTATgc                                                                     >  1:1224109/1‑93 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgc                                                                     <  2:4556935/93‑1 (MQ=255)

                    aTTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgcg                                                                    >  1:1318995/1‑93 (MQ=255)

                     ttCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgcgc                                                                   <  1:341920/93‑1 (MQ=255)

                      tCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgcc                                                                  >  1:1007635/1‑93 (MQ=255)

                        accacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgccgc                                                                >  2:4576725/1‑93 (MQ=255)

                         ccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCa                                                               >  1:798981/1‑93 (MQ=255)

                         ccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTCTGCGCCGCa                                                               >  1:2727748/1‑93 (MQ=255)

                         ccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCa                                                               >  1:2727747/1‑93 (MQ=255)

                              cagACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGAATGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGAt                                                          <  2:4193920/91‑1 (MQ=255)

                              cTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGAt                                                          >  1:1787065/1‑93 (MQ=255)

                              cTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGAt                                                          >  1:4749535/1‑93 (MQ=255)

                               tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCGCGCTTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATc                                                         <  2:2431933/93‑1 (MQ=255)

                               tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAGCGGCGCGGACTATGCGCCGCATGGATc                                                         <  2:1718435/93‑1 (MQ=255)

                               tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATc                                                         <  1:3326191/93‑1 (MQ=255)

                                  ccAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCa                                                      >  1:4098581/1‑93 (MQ=255)

                                    taagaagCATTGCCGGTCATTAGCTACCGCACGCGTGATTTGACGCGACTGTTATCAGGAACGGCGCGGACTATGCGGCGCATGGATCGCATc                                                    >  2:2953591/2‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                    <  2:805763/93‑1 (MQ=255)

                                     aagaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCa                                                   >  1:1066017/1‑93 (MQ=255)

                                      agaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAg                                                  >  2:4077709/1‑93 (MQ=255)

                                       gaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGc                                                 >  2:549530/1‑93 (MQ=255)

                                        aagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCg                                                <  1:1259710/93‑1 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                              <  1:3495870/93‑1 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                              <  2:540984/93‑1 (MQ=255)

                                           cATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGAc                                             >  2:3395477/1‑93 (MQ=255)

                                           cATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGAc                                             <  2:3655001/93‑1 (MQ=255)

                                              tGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCa                                          <  1:4490210/93‑1 (MQ=255)

                                                ccGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGc                                        <  1:785656/93‑1 (MQ=255)

                                                ccGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGc                                        <  1:4552016/93‑1 (MQ=255)

                                                 cGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCGTGGATCGCATCAGCGGACGCAGCg                                       >  1:3789463/1‑93 (MQ=255)

                                                 cGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCg                                       >  2:82880/1‑93 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                      >  1:974017/1‑93 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                      <  2:3281807/93‑1 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                      <  2:649091/93‑1 (MQ=255)

                                                  cgTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                      <  2:1594393/92‑1 (MQ=255)

                                                   gTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGAt                                     >  2:3890624/1‑93 (MQ=255)

                                                     cATTCGTTCCCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                   <  1:3839103/93‑1 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                   >  1:213999/1‑93 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                   >  1:2305463/1‑93 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                   >  2:1844676/1‑93 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                   <  1:2586067/93‑1 (MQ=255)

                                                      aTTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGAc                                  >  2:1431915/1‑93 (MQ=255)

                                                      aTTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGAc                                  >  1:1141174/1‑93 (MQ=255)

                                                       ttCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACa                                 <  2:3321356/93‑1 (MQ=255)

                                                        tCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACAt                                <  2:1569135/93‑1 (MQ=255)

                                                         cGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATg                               >  1:2381418/1‑93 (MQ=255)

                                                          gTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGt                              <  2:2785930/93‑1 (MQ=255)

                                                          gTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGACGCATGGATCGCATCAGCGGACGCAGCGATGACATGt                              >  2:1149079/1‑93 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                             >  1:1772711/1‑93 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                             >  1:4470902/1‑93 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                             <  1:2860775/93‑1 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                             >  2:3520550/1‑93 (MQ=255)

                                                            tACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTg                            >  2:2062821/1‑93 (MQ=255)

                                                             aCCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGa                           >  2:2243911/1‑93 (MQ=255)

                                                              ccGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGat                          >  1:445832/1‑93 (MQ=255)

                                                               cGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatt                         <  2:269493/93‑1 (MQ=255)

                                                                gCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatta                        <  2:3827441/93‑1 (MQ=255)

                                                                gCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatta                        <  1:2969306/93‑1 (MQ=255)

                                                                   cgcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTc                     <  1:4470039/93‑1 (MQ=255)

                                                                   cgcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTc                     <  1:2000485/93‑1 (MQ=255)

                                                                    gcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGGGATGACATGTTGATTATTcg                    >  1:1178392/1‑93 (MQ=255)

                                                                    gcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                    <  2:4633601/93‑1 (MQ=255)

                                                                     cgTGATTTACCCCGAATGTTATCAGGAAAGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgc                   <  2:136392/93‑1 (MQ=255)

                                                                     cgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgc                   <  1:907170/93‑1 (MQ=255)

                                                                       tGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCgg                 >  2:1834044/1‑93 (MQ=255)

                                                                       tGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCgg                 >  1:2526910/1‑93 (MQ=255)

                                                                        gATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgt                <  1:2007867/93‑1 (MQ=255)

                                                                        gATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgt                <  1:1191819/93‑1 (MQ=255)

                                                                         aTTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtg               <  1:3098074/93‑1 (MQ=255)

                                                                         aTTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtg               <  2:3590743/93‑1 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt              >  2:1005547/1‑93 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt              >  2:3289745/1‑93 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt              <  1:2137809/93‑1 (MQ=255)

                                                                           ttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa             >  1:200776/1‑93 (MQ=255)

                                                                            tAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTaa            >  1:3628310/1‑93 (MQ=255)

                                                                              aCCAGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt          <  1:3873070/93‑1 (MQ=255)

                                                                               cccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATg         >  1:2811538/1‑93 (MQ=255)

                                                                               cccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATg         <  1:3274761/93‑1 (MQ=255)

                                                                                ccGACTGTTATCAGGGACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGt        <  1:836088/93‑1 (MQ=255)

                                                                                 cGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTc       >  1:2839527/1‑93 (MQ=255)

                                                                                   aCTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt     <  1:1687703/93‑1 (MQ=255)

                                                                                    atGTTAACAGGAACGGCGCGAAATATGCGGCGCATGGATCGCATCAGCGGACGAAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtat    <  2:1492082/92‑3 (MQ=255)

                                                                                     tGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTc   <  1:3435967/93‑1 (MQ=255)

                                                                                      gTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTcc  <  2:1482016/93‑1 (MQ=255)

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCC  >  NC_000913/1460992‑1461170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`