Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,462,362 T→C I267T (ATT→ACT)  b1399 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,462,3620TC97.1% 423.6 / ‑0.6 137I267T (ATT→ACT) b1399hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base C (75/58);  total (75/62)
Fisher's exact test for biased strand distribution p-value = 3.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 148 total aligned reads displayed.

TCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACATTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGT  >  NC_000913/1462270‑1462451
                                                                                            |                                                                                         
tCGCCGTGTCGTCCTTTAAGACCCATTGTTGCCGGAGTAGTTGCTTTCGGCACACTGGTGAGGGCATACGGAGCGTCTGCTGTGTATGAACAt                                                                                           >  2:3183378/1‑93 (MQ=255)
  gCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACActt                                                                                         <  1:2915039/93‑1 (MQ=255)
  gCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACActt                                                                                         >  2:2978724/1‑93 (MQ=255)
   ccGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTa                                                                                        <  2:1469471/93‑1 (MQ=255)
     gtgtCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATc                                                                                      <  2:2052773/93‑1 (MQ=255)
      tgtCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCa                                                                                     >  1:475506/1‑93 (MQ=255)
        tcgtcCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAgc                                                                                   >  2:772064/1‑93 (MQ=255)
        tcgtcCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAgc                                                                                   <  1:700438/93‑1 (MQ=255)
          gtcCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAgcgc                                                                                 <  1:4106703/93‑1 (MQ=255)
           tcCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGcgcg                                                                                >  2:1348294/1‑93 (MQ=255)
             cTTAAAGACCCATTGTTTCCGGAGGAGTTGCTTCCGGCACACTGGGCATGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTa                                                                              >  2:385857/1‑93 (MQ=255)
             cTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTa                                                                              >  1:3329819/1‑93 (MQ=255)
             cTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTa                                                                              >  2:4110028/1‑93 (MQ=255)
               tAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAgc                                                                            <  2:3271948/93‑1 (MQ=255)
               tAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAgc                                                                            <  2:300219/93‑1 (MQ=255)
                aaaGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACATTTATCAGCGCGTAgcg                                                                           <  2:3492633/93‑1 (MQ=255)
                 aaGACCCATTTTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTGATCAGCGCGTAgcgc                                                                          >  2:4222252/1‑93 (MQ=255)
                 aaGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAgcgc                                                                          >  1:4257469/1‑93 (MQ=255)
                 aaGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAgcgc                                                                          >  1:1657700/1‑93 (MQ=255)
                  aGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACATTTATCAGCGCGTAGCGcc                                                                         <  1:3135710/93‑1 (MQ=255)
                  aGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGcc                                                                         >  2:1368696/1‑93 (MQ=255)
                  aGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGcc                                                                         <  2:461996/93‑1 (MQ=255)
                  aGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGcc                                                                         <  1:425223/93‑1 (MQ=255)
                  aGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGcc                                                                         >  2:2657743/1‑93 (MQ=255)
                   gACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCct                                                                        <  1:102904/93‑1 (MQ=255)
                   gACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCct                                                                        <  1:4451269/93‑1 (MQ=255)
                   gACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCct                                                                        >  1:3107720/1‑93 (MQ=255)
                    aCCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctg                                                                       >  1:3571512/1‑93 (MQ=255)
                    aCCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctg                                                                       >  2:2531659/1‑93 (MQ=255)
                    aCCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctg                                                                       >  1:3167740/1‑93 (MQ=255)
                     cccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgc                                                                      >  1:985709/1‑93 (MQ=255)
                     cccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgc                                                                      >  2:2709026/1‑93 (MQ=255)
                     cccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgc                                                                      >  2:3971461/1‑93 (MQ=255)
                      ccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgct                                                                     >  1:2441026/1‑93 (MQ=255)
                      ccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgct                                                                     >  1:115530/1‑93 (MQ=255)
                      ccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgct                                                                     >  1:822124/1‑93 (MQ=255)
                      ccATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgct                                                                     >  1:4665670/1‑93 (MQ=255)
                       cATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgctg                                                                    >  2:2183603/1‑93 (MQ=255)
                       cATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCctgctg                                                                    >  2:2170751/1‑93 (MQ=255)
                        aTTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCtgctgc                                                                   >  1:1875321/1‑93 (MQ=255)
                        aTTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCtgctgc                                                                   >  1:1221601/1‑93 (MQ=255)
                        aTTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCtgctgc                                                                   >  1:3701964/1‑93 (MQ=255)
                         ttgttgCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTgctgct                                                                  >  2:4147128/1‑93 (MQ=255)
                            ttgCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTa                                                               >  1:1565104/1‑93 (MQ=255)
                            ttgCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTa                                                               <  2:368116/93‑1 (MQ=255)
                             tgCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAg                                                              <  1:416044/93‑1 (MQ=255)
                             tgCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAg                                                              <  2:3148542/93‑1 (MQ=255)
                              gCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGc                                                             <  2:4057196/93‑1 (MQ=255)
                                 ggaggaGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGcgtt                                                          <  1:2801433/93‑1 (MQ=255)
                                  gaggagTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGcgttc                                                         <  1:4348673/93‑1 (MQ=255)
                                   aggagTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGcgttcg                                                        <  2:3850999/93‑1 (MQ=255)
                                      agTGGCTTCCGCCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTa                                                     <  2:1899749/93‑1 (MQ=255)
                                       gTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAg                                                    >  1:3302580/1‑93 (MQ=255)
                                        ttGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGt                                                   >  1:3268287/1‑93 (MQ=255)
                                        ttGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGt                                                   <  2:1571897/93‑1 (MQ=255)
                                         tGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTg                                                  <  1:2414849/93‑1 (MQ=255)
                                           cTTCCGGCACACTGGGCAGGGCATACGGCGCCGCAGCTCGGAATCAACAGTTATCAGGGCGTAGCCGCTGCTGCTTTAGTGTTCGTTAGTGaa                                                >  2:4046730/1‑93 (MQ=255)
                                             tCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGaaaa                                              >  1:2150623/1‑93 (MQ=255)
                                             tCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGAGCGTAGCGACTGCTGATTTAGCGTTCGTTAGTGaaaa                                              >  2:337774/1‑93 (MQ=255)
                                              ccGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGaaaaa                                             >  2:1045967/1‑93 (MQ=255)
                                                ggCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAgg                                           <  1:2292359/93‑1 (MQ=255)
                                                  cacaCTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTg                                         >  1:25585/1‑93 (MQ=255)
                                                  cacaCTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTg                                         <  2:3362110/93‑1 (MQ=255)
                                                  cacaCTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTg                                         <  1:27888/93‑1 (MQ=255)
                                                  cacaCTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTg                                         >  1:25586/1‑93 (MQ=255)
                                                   acacTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGa                                        >  1:3191095/1‑93 (MQ=255)
                                                   acacTGGGCAGGGCATAAGGCGCGTCAGCTGTGTATCAACACTTATCCGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAACAGgaga                                        >  1:3564543/1‑93 (MQ=255)
                                                    cacTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGGGAGAAAGGTGaa                                       >  2:1236635/1‑93 (MQ=255)
                                                    cacTGGGCAGAGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGaa                                       <  2:4359962/93‑1 (MQ=255)
                                                      cTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAAc                                     >  1:3774677/1‑93 (MQ=255)
                                                      cTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAAc                                     >  2:2847330/1‑93 (MQ=255)
                                                         ggcagggcaTACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCtc                                  <  2:2403499/93‑1 (MQ=255)
                                                          gcagggcaTACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCtcg                                 <  2:372272/93‑1 (MQ=255)
                                                            agggcaTACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCtcggt                               <  2:1811722/93‑1 (MQ=255)
                                                             gggcaTACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCtcggtc                              >  2:1173512/1‑93 (MQ=255)
                                                                 aTACGGCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTg                          >  2:523415/1‑93 (MQ=255)
                                                                     ggCGCGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAAct                      >  2:2743849/1‑93 (MQ=255)
                                                                      gcgcGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActg                     >  2:3470023/1‑93 (MQ=255)
                                                                      gcgcGTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActg                     >  2:1232002/1‑93 (MQ=255)
                                                                        gcgTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcc                   <  1:209471/93‑1 (MQ=255)
                                                                        gcgTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcc                   <  1:128108/93‑1 (MQ=255)
                                                                         cgTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcct                  <  1:1726052/93‑1 (MQ=255)
                                                                         cgTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcct                  >  2:1425862/1‑93 (MQ=255)
                                                                          gTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcctg                 <  1:4045064/93‑1 (MQ=255)
                                                                          gTCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcctg                 >  1:515257/1‑93 (MQ=255)
                                                                           tCAGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAActgcctgc                >  1:899370/1‑93 (MQ=255)
                                                                             cgCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTgcgc              <  1:854871/92‑1 (MQ=255)
                                                                             aGCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTgcgc              >  1:3130597/1‑93 (MQ=255)
                                                                              gCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGcc             >  2:2266611/1‑93 (MQ=255)
                                                                              gCTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGcc             >  2:1644851/1‑93 (MQ=255)
                                                                               cTGTGTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCg            <  1:3872730/93‑1 (MQ=255)
                                                                                tgtgTATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCgg           <  1:4609887/93‑1 (MQ=255)
                                                                                  tgtATCAACACTTATCATCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGa         <  2:2914426/93‑1 (MQ=255)
                                                                                    tATCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAg       >  2:4450183/1‑93 (MQ=255)
                                                                                     aTCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGc      <  2:3530744/93‑1 (MQ=255)
                                                                                     aTCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGc      <  2:3473061/93‑1 (MQ=255)
                                                                                      tCAACACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGcc     >  1:1003547/1‑93 (MQ=255)
                                                                                        aaCATTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTg   <  2:735657/93‑1 (MQ=255)
                                                                                        aaCACTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTg   <  1:2331100/93‑1 (MQ=255)
                                                                                         acacTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGt  >  2:205448/1‑93 (MQ=255)
                                                                                            |                                                                                         
TCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACATTTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGT  >  NC_000913/1462270‑1462451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑