BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,473,746	A→G	K195K (AAA→AAG)	ydbD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,473,746	0	A	G	99.5%	638.4 / ‑5.8	196	K195K (AAA→AAG)	ydbD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (80/115); total (80/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 205 total aligned reads displayed.

TGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCAATAT  >  NC_000913/1473656‑1473835

tGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGgc                                                                                         >  2:3275818/1‑93 (MQ=255)

 gACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGgcg                                                                                        >  2:2293767/1‑93 (MQ=255)

  aCATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGgcgc                                                                                       <  2:1929797/93‑1 (MQ=255)

  aCATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGgcgc                                                                                       <  2:1316756/93‑1 (MQ=255)

    aTAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtg                                                                                     >  2:2929549/1‑93 (MQ=255)

    aTAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtg                                                                                     <  2:4432815/93‑1 (MQ=255)

     tAATTGCTCCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtga                                                                                    <  2:824661/93‑1 (MQ=255)

     tAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtga                                                                                    <  2:939774/93‑1 (MQ=255)

     tAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtga                                                                                    >  2:643506/1‑93 (MQ=255)

     tAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtga                                                                                    >  1:3784152/1‑93 (MQ=255)

      aaTTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtgat                                                                                   <  2:2427994/93‑1 (MQ=255)

       aTTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCtgatg                                                                                  <  1:968507/93‑1 (MQ=255)

         tGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTG‑AAATGTCAAAAAGGCGCTGATGata                                                                               <  2:2179216/93‑1 (MQ=255)

            cccTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGataat                                                                             <  2:2162108/92‑1 (MQ=255)

                 ttgttgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATc                                                                        >  1:4392627/1‑93 (MQ=255)

                  tgttgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATcc                                                                       <  2:3516524/93‑1 (MQ=255)

                   gttgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCt                                                                      >  1:1342662/1‑93 (MQ=255)

                   gttgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCt                                                                      <  2:3821179/93‑1 (MQ=255)

                    ttgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTg                                                                     >  1:605919/1‑93 (MQ=255)

                     tgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGa                                                                    >  2:4233835/1‑93 (MQ=255)

                     tgCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGa                                                                    <  2:2667405/93‑1 (MQ=255)

                      gCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGAt                                                                   <  2:4626372/93‑1 (MQ=255)

                       cGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATg                                                                  <  1:1506568/93‑1 (MQ=255)

                         tCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCa                                                                <  2:599742/93‑1 (MQ=255)

                         tCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCa                                                                >  1:3567588/1‑93 (MQ=255)

                         tCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCa                                                                <  1:3610401/93‑1 (MQ=255)

                           ttCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCata                                                              >  1:2395696/1‑93 (MQ=255)

                           ttCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCata                                                              >  2:2967600/1‑93 (MQ=255)

                            tCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCatat                                                             >  2:1240542/1‑93 (MQ=255)

                            tCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCatat                                                             >  2:4665009/1‑93 (MQ=255)

                            tCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTC‑AAAAGGCGCTGATGATAATAAATCCTGATGCAtatt                                                            <  2:3379147/93‑1 (MQ=255)

                             cGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCAtatt                                                            <  2:4286772/93‑1 (MQ=255)

                              gACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCAtatta                                                           <  1:1276771/93‑1 (MQ=255)

                               aCAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCAtattat                                                          <  1:3380719/93‑1 (MQ=255)

                               aCAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCAtattat                                                          <  2:4591792/93‑1 (MQ=255)

                                cAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATattatt                                                         >  2:657104/1‑93 (MQ=255)

                                cAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATattatt                                                         >  1:1984814/1‑93 (MQ=255)

                                 aaaTAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTg                                                        <  2:407316/93‑1 (MQ=255)

                                 aaaTAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTg                                                        <  1:255564/93‑1 (MQ=255)

                                  aaTAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTgg                                                       <  1:278121/93‑1 (MQ=255)

                                   aTAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTggg                                                      <  2:4121575/93‑1 (MQ=255)

                                    tAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGTCGCTGATGATAATAAATCCTGATGCATATTATTgggg                                                     >  1:3314409/1‑93 (MQ=255)

                                      gTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGc                                                   >  1:3041897/1‑93 (MQ=255)

                                        gAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCta                                                 >  1:1042426/1‑93 (MQ=255)

                                         aaTTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaa                                                >  2:22410/1‑93 (MQ=255)

                                          aTTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaat                                               <  2:2674309/93‑1 (MQ=255)

                                          aTTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaat                                               <  1:2456016/93‑1 (MQ=255)

                                             cATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaattaa                                            >  1:3678622/1‑93 (MQ=255)

                                             cATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaattaa                                            <  1:1677641/93‑1 (MQ=255)

                                             cATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaattaa                                            <  2:390948/93‑1 (MQ=255)

                                              aTGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaattaat                                           <  1:1132502/93‑1 (MQ=255)

                                               tGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAatg                                          <  2:849364/93‑1 (MQ=255)

                                                 gTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAatgat                                        >  2:1862236/1‑93 (MQ=255)

                                                  tCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAatgatg                                       <  1:3255809/93‑1 (MQ=255)

                                                  tCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAatgatg                                       >  1:329914/1‑93 (MQ=255)

                                                       gCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGAt                                  <  2:3043310/93‑1 (MQ=255)

                                                       gCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGAt                                  <  2:449020/93‑1 (MQ=255)

                                                       gCGAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGAt                                  <  2:2739958/93‑1 (MQ=255)

                                                         gAGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGc                                <  1:2309493/93‑1 (MQ=255)

                                                          aGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGct                               <  2:3669386/93‑1 (MQ=255)

                                                          aGTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGct                               >  1:3112246/1‑93 (MQ=255)

                                                           gTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGctc                              >  2:2017204/1‑93 (MQ=255)

                                                           gTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGctc                              >  2:3592211/1‑93 (MQ=255)

                                                           gTGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGctc                              <  2:1822526/93‑1 (MQ=255)

                                                            tGACAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGctct                             <  1:1156206/93‑1 (MQ=255)

                                                              aCAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTga                           >  2:3416923/1‑93 (MQ=255)

                                                               cAAACATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaa                          >  2:3322069/1‑93 (MQ=255)

                                                                aaaCATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaa                         >  1:2194440/1‑93 (MQ=255)

                                                                aaaCATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaa                         <  2:490779/93‑1 (MQ=255)

                                                                 aaCATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaag                        >  2:4313784/1‑93 (MQ=255)

                                                                 aaCATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaag                        >  2:1686445/1‑93 (MQ=255)

                                                                  aCATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaaga                       >  1:4503613/1‑93 (MQ=255)

                                                                   cATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaagaa                      >  2:154364/1‑93 (MQ=255)

                                                                   cATGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaagaa                      <  2:3439932/93‑1 (MQ=255)

                                                                    aTGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaagaaa                     >  1:2864031/1‑93 (MQ=255)

                                                                    aTGACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTgaaagaaa                     <  2:563642/93‑1 (MQ=255)

                                                                      gACACTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAAtt                   >  1:373865/1‑93 (MQ=255)

                                                                       acacTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAAttt                  >  2:2760458/1‑93 (MQ=255)

                                                                        cacTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAAtttt                 >  1:4470482/1‑93 (MQ=255)

                                                                        cacTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAAtttt                 >  2:2991552/1‑93 (MQ=255)

                                                                         acTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAAttttt                >  1:2180295/1‑93 (MQ=255)

                                                                          cTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTa               <  1:763898/93‑1 (MQ=255)

                                                                          cTGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTa               >  2:3693779/1‑93 (MQ=255)

                                                                           tGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTaa              <  1:101094/93‑1 (MQ=255)

                                                                           tGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTaa              >  2:947568/1‑93 (MQ=255)

                                                                           tGAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTaa              <  1:4191776/93‑1 (MQ=255)

                                                                            gAAAATGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAg             >  1:1395622/1‑93 (MQ=255)

                                                                             aaaaTGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTGATGATGAAGATGCTCTGAAAGAAATTTTTAAGc            >  1:4565473/1‑93 (MQ=255)

                                                                             aaaaTGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGc            >  2:1071893/1‑93 (MQ=255)

                                                                             aaaaTGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGc            <  1:4699899/93‑1 (MQ=255)

                                                                             aaaaTGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGc            <  1:179941/93‑1 (MQ=255)

                                                                                aTGTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGaa         >  1:2124709/1‑93 (MQ=255)

                                                                                 tGTCAAAAAGGAGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAAGGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAg        <  1:2625555/93‑1 (MQ=255)

                                                                                  gTCAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGc       <  2:3121742/93‑1 (MQ=255)

                                                                                    cAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCaa     <  2:2843162/93‑1 (MQ=255)

                                                                                    cAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCaa     <  1:2277906/93‑1 (MQ=255)

                                                                                    cAAAAAGGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGCCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCaa     <  2:1329973/93‑1 (MQ=255)

                                                                                     aaaaagGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCAat    >  1:802603/1‑93 (MQ=255)

                                                                                      aaaagGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCAata   <  1:400418/93‑1 (MQ=255)

                                                                                       aaagGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCAatat  <  2:1764634/93‑1 (MQ=255)

TGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTAATGATGAAGATGCTCTGAAAGAAATTTTTAAGCGAAGCAATAT  >  NC_000913/1473656‑1473835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`