BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,478,094	A→G	G311G (GGA→GGG)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,094	0	A	G	98.8%	524.5 / ‑3.3	169	G311G (GGA→GGG)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (83/83); minor base C (0/2); total (83/86)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

Only 100 of 177 total aligned reads displayed.

TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183

tttATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGcc                                                                                         <  1:792140/93‑1 (MQ=255)

  tataTGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACCTTCTGGTGGGCCCg                                                                                       >  2:1979874/1‑93 (MQ=255)

  tataTGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACCTTCTGGTGGGCCCg                                                                                       >  2:1979875/1‑93 (MQ=255)

      tGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGcc                                                                                   >  2:1613986/1‑93 (MQ=255)

        cTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTAATTTCTGGAGGGCCCGTGCCAt                                                                                 >  1:1319030/1‑93 (MQ=255)

         tAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCATAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCAtt                                                                                >  1:1233087/1‑93 (MQ=255)

         tAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCAtt                                                                                >  1:2706959/1‑93 (MQ=255)

          aaCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTa                                                                               >  2:2465661/1‑93 (MQ=255)

            cAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATc                                                                             <  2:1187801/93‑1 (MQ=255)

             aaaTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATcc                                                                            >  2:2670853/1‑92 (MQ=255)

              aaTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGCCCCGTGCCATTATCGc                                                                           <  2:3970174/93‑1 (MQ=255)

               aTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGcc                                                                          <  1:311151/93‑1 (MQ=255)

               aTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGcc                                                                          <  2:421878/93‑1 (MQ=255)

                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  2:1773310/93‑1 (MQ=255)

                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  1:2254125/93‑1 (MQ=255)

                    aaaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCgttga                                                                     >  1:4426555/1‑89 (MQ=255)

                    aaaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTg                                                                     >  2:4746494/1‑93 (MQ=255)

                    aaaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTg                                                                     <  1:3052558/93‑1 (MQ=255)

                    aaaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGACATTATCGCCGGTTg                                                                     >  2:3641308/1‑93 (MQ=255)

                     aaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGa                                                                    >  2:4683122/1‑93 (MQ=255)

                     aaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGa                                                                    >  1:2575212/1‑93 (MQ=255)

                       aaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGAtt                                                                  <  1:1619105/93‑1 (MQ=255)

                       aaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGAtt                                                                  <  1:4040155/93‑1 (MQ=255)

                        aTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGAttt                                                                 >  1:4388366/1‑93 (MQ=255)

                         tttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTa                                                                >  2:1156033/1‑93 (MQ=255)

                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               <  1:4632251/93‑1 (MQ=255)

                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               <  1:1795864/93‑1 (MQ=255)

                           tGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGc                                                              >  1:1436985/1‑93 (MQ=255)

                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             >  1:2180943/1‑93 (MQ=255)

                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             >  2:1400581/1‑93 (MQ=255)

                              ttATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                           >  1:4738305/1‑93 (MQ=255)

                              ttATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                           >  1:547216/1‑93 (MQ=255)

                              ttATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                           >  1:3085212/1‑93 (MQ=255)

                                  cAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGc                                                       <  2:2770463/93‑1 (MQ=255)

                                  cAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGc                                                       <  2:355526/93‑1 (MQ=255)

                                  cAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGc                                                       <  2:4139641/93‑1 (MQ=255)

                                   aGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACATTCTGGTGGGCCCGTGCCATTATCGCCGGGTGATTTAGTGTATCGCt                                                      >  2:4470747/1‑93 (MQ=255)

                                    gCATGAAGACTGCACAGGACCAGCAGCGGATCGAAGGCGGCTACCCTCTGGTGGGCCCGTGCCACTATCGCCGGTTGACTTAGCGTATTGCtt                                                     >  1:3618231/1‑93 (MQ=255)

                                     cATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCttt                                                    <  2:962586/93‑1 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  2:3926307/1‑93 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  <  1:896770/93‑1 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  2:2314845/1‑93 (MQ=255)

                                        gAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATg                                                 >  2:2411399/1‑93 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                <  2:3463788/93‑1 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                <  1:3317262/93‑1 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                >  1:321790/1‑93 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                <  2:1686196/93‑1 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                >  2:1569377/1‑93 (MQ=255)

                                            aCTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaa                                             <  2:2942028/93‑1 (MQ=255)

                                             cTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaaa                                            >  2:1808048/1‑93 (MQ=255)

                                             cTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaaa                                            <  1:4507979/93‑1 (MQ=255)

                                             cTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaaa                                            <  2:4596510/93‑1 (MQ=255)

                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           <  1:3984522/93‑1 (MQ=255)

                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           >  2:2820269/1‑93 (MQ=255)

                                                 acaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCg                                        >  2:3825859/1‑93 (MQ=255)

                                                   aGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGAt                                      >  1:3188404/1‑93 (MQ=255)

                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  2:1716655/1‑93 (MQ=255)

                                                        cATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAt                                 >  2:1066771/1‑93 (MQ=255)

                                                        cATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAt                                 <  2:3403045/93‑1 (MQ=255)

                                                         aTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAtt                                <  2:3598774/93‑1 (MQ=255)

                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               >  1:673681/1‑93 (MQ=255)

                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               <  1:2484505/93‑1 (MQ=255)

                                                            aGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGtt                             >  1:2573676/1‑93 (MQ=255)

                                                             gCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTCTCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                            >  2:268948/1‑93 (MQ=255)

                                                              cGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCg                           <  2:3932356/93‑1 (MQ=255)

                                                               ggATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgg                          >  1:1092336/1‑93 (MQ=255)

                                                               ggATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgg                          >  2:6096/1‑93 (MQ=255)

                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTTCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         <  1:847876/93‑1 (MQ=255)

                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         >  2:559968/1‑93 (MQ=255)

                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         <  1:847877/93‑1 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  1:3336775/93‑1 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        >  2:3640833/1‑93 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  2:1011500/93‑1 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        >  2:4419208/1‑93 (MQ=255)

                                                                    gATGGCGGCTACTTTCTGGTGGGCTCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGc                     <  2:1707136/93‑1 (MQ=255)

                                                                      tGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCAc                   <  1:1953006/93‑1 (MQ=255)

                                                                       ggcggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCAct                  >  1:420562/1‑93 (MQ=255)

                                                                        gcggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCActc                 <  2:2919575/93‑1 (MQ=255)

                                                                        gcggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCActc                 <  1:572296/93‑1 (MQ=255)

                                                                         cggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCActct                <  2:451278/93‑1 (MQ=255)

                                                                         cggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCActct                <  2:3519784/93‑1 (MQ=255)

                                                                          ggcTCCTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCGCTCtt               <  2:4281645/93‑1 (MQ=255)

                                                                          ggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCtt               <  2:4103334/93‑1 (MQ=255)

                                                                          ggcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCtt               >  2:4543693/1‑93 (MQ=255)

                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTc              <  2:1344636/93‑1 (MQ=255)

                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTc              <  1:141331/93‑1 (MQ=255)

                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTc              >  2:3766065/1‑93 (MQ=255)

                                                                             tACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCgg            <  1:1977705/93‑1 (MQ=255)

                                                                             tACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCgg            >  1:297440/1‑93 (MQ=255)

                                                                              tcTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCggg           <  2:3040617/92‑1 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         >  1:2317457/1‑93 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         >  1:4291939/1‑93 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         <  1:1512891/93‑1 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         >  1:3541632/1‑93 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         <  1:1971820/93‑1 (MQ=255)

                                                                                   cTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGc      >  1:2311535/1‑93 (MQ=255)

                                                                                    tggtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCa     >  2:400596/1‑93 (MQ=255)

                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAtc   <  1:2378170/93‑1 (MQ=255)

                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAtc   >  2:3101449/1‑93 (MQ=255)

                                                                                       tggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAtct  <  2:148671/93‑1 (MQ=255)

TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`