Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,838 A→G W249R (TGG→CGG)  speD ← S‑adenosylmethionine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,8380AG99.4% 562.0 / NA 173W249R (TGG→CGG) speDS‑adenosylmethionine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (108/64);  minor base T (1/0);  total (109/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

GTAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAA  >  NC_000913/134747‑134927
                                                                                           |                                                                                         
    ataaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcg                                                                                      >  1:354078/1‑93 (MQ=255)
     taaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcgc                                                                                     >  1:3690450/1‑93 (MQ=255)
     taaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcgc                                                                                     >  1:4061437/1‑93 (MQ=255)
     taaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGcagcgc                                                                                     >  1:3049991/1‑93 (MQ=255)
       aatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCcgcagcgcag                                                                                   >  1:3325396/1‑93 (MQ=255)
       aatCTGGCGGACCCTGGGGGCGCCACCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCcgcagcgcag                                                                                   <  1:3477477/93‑1 (MQ=255)
          ttGGCAGAGCCGGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCgg                                                                                <  1:3232545/92‑1 (MQ=255)
           tGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCgga                                                                               >  1:1663577/1‑92 (MQ=255)
           tGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGt                                                                               >  1:4308164/1‑93 (MQ=255)
             gCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  2:2413450/93‑1 (MQ=255)
             gCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTaa                                                                             <  2:673901/93‑1 (MQ=255)
                gAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAAttt                                                                          >  2:387639/1‑93 (MQ=255)
                 aGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTc                                                                         >  2:3045254/1‑93 (MQ=255)
                  gCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTcc                                                                        >  1:1957872/1‑93 (MQ=255)
                   ccTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCt                                                                       <  1:4622168/93‑1 (MQ=255)
                   ccTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCt                                                                       >  2:2756598/1‑93 (MQ=255)
                    cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTg                                                                      >  2:2022914/1‑93 (MQ=255)
                    cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTg                                                                      <  1:2774968/93‑1 (MQ=255)
                    cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTg                                                                      <  1:4680582/93‑1 (MQ=255)
                     tGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCTCATCGCAGCGGCAATTTCCTgg                                                                     >  2:4710872/1‑93 (MQ=255)
                     tGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTgg                                                                     >  2:4630729/1‑93 (MQ=255)
                      gggAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgc                                                                    >  2:3831651/1‑93 (MQ=255)
                      gggAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgc                                                                    >  2:3748790/1‑93 (MQ=255)
                       ggAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGgcg                                                                   <  1:1024832/93‑1 (MQ=255)
                         ggCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgct                                                                 <  1:4135643/92‑1 (MQ=255)
                         aGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgct                                                                 <  2:4583108/93‑1 (MQ=255)
                         aGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgct                                                                 <  1:4501018/93‑1 (MQ=255)
                            tCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgctcgc                                                              >  1:2578232/1‑93 (MQ=255)
                             ccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGcgctcgct                                                             <  1:2968227/93‑1 (MQ=255)
                              cGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTg                                                            >  1:516487/1‑93 (MQ=255)
                                ccAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTc                                                          >  1:3158125/1‑93 (MQ=255)
                                 cAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCg                                                         <  2:147852/93‑1 (MQ=255)
                                   gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGt                                                       >  1:3098375/1‑93 (MQ=255)
                                    agCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGtt                                                      <  2:3419251/93‑1 (MQ=255)
                                      ccGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTaa                                                    <  2:2351923/93‑1 (MQ=255)
                                      ccGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTaa                                                    <  2:3109734/93‑1 (MQ=255)
                                       cGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAg                                                   >  2:2275010/1‑93 (MQ=255)
                                        gTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGt                                                  >  1:2875124/1‑93 (MQ=255)
                                         ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 <  1:4393528/93‑1 (MQ=255)
                                         ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 >  2:4167859/1‑93 (MQ=255)
                                         ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 >  1:387669/1‑93 (MQ=255)
                                         ttAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                                                 <  2:232157/93‑1 (MQ=255)
                                           aaaCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCtt                                               <  1:2167740/93‑1 (MQ=255)
                                            aaCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTc                                              <  2:4104312/93‑1 (MQ=255)
                                             aCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTcc                                             >  1:3360829/1‑93 (MQ=255)
                                             aCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTcc                                             <  1:3127344/93‑1 (MQ=255)
                                             aCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTcc                                             >  2:2502580/1‑93 (MQ=255)
                                               aGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCgg                                           >  1:2906401/1‑93 (MQ=255)
                                               aGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCgg                                           >  1:2350517/1‑93 (MQ=255)
                                                gCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGt                                          <  1:1674296/93‑1 (MQ=255)
                                                gCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGt                                          <  1:1527034/93‑1 (MQ=255)
                                                  ccgCATATTGCGCCCGGAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGttt                                        <  2:1275383/91‑1 (MQ=255)
                                                   ggCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTGCGGTTTg                                       >  1:640935/1‑93 (MQ=255)
                                                    gCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTGGCTGTCGGTTAAGTCTTCCGGTTTgg                                      <  1:2062508/93‑1 (MQ=255)
                                                      atatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTCATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtg                                    >  1:3155174/1‑93 (MQ=255)
                                                      atatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtg                                    <  2:1035598/93‑1 (MQ=255)
                                                      atatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtg                                    <  1:592455/93‑1 (MQ=255)
                                                       tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   <  2:236783/93‑1 (MQ=255)
                                                       tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   <  2:537787/93‑1 (MQ=255)
                                                       tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   <  2:4724616/93‑1 (MQ=255)
                                                       tatTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt                                   >  1:4587787/1‑93 (MQ=255)
                                                        atTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGtgtg                                  >  2:2126131/1‑93 (MQ=255)
                                                         ttGCGCCCGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTgg                                 >  1:105634/1‑93 (MQ=255)
                                                               ccGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATg                           >  2:4426144/1‑93 (MQ=255)
                                                                cGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGt                          >  2:208796/1‑93 (MQ=255)
                                                                cGTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGt                          >  1:4478654/1‑93 (MQ=255)
                                                                 gTAATAAATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTa                         >  2:113414/1‑93 (MQ=255)
                                                                  taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        <  1:300300/93‑1 (MQ=255)
                                                                  taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        <  1:4034219/93‑1 (MQ=255)
                                                                  taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        >  1:4700528/1‑93 (MQ=255)
                                                                  taataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAg                        >  1:4514536/1‑93 (MQ=255)
                                                                   aataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGt                       <  2:2340431/93‑1 (MQ=255)
                                                                    ataaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTg                      >  1:1605511/1‑93 (MQ=255)
                                                                     taaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGc                     >  1:4172595/1‑93 (MQ=255)
                                                                     taaATCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGc                     >  1:4172596/1‑93 (MQ=255)
                                                                      aaaTCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCt                    <  2:2912289/93‑1 (MQ=255)
                                                                       aaTCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCtt                   <  2:4133852/93‑1 (MQ=255)
                                                                        aTCTCGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTa                  >  1:1275419/1‑93 (MQ=255)
                                                                         tctcGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTaa                 >  2:1642316/1‑93 (MQ=255)
                                                                         tctcGCGCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTaa                 >  1:346103/1‑93 (MQ=255)
                                                                            cgcgCATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGt              <  1:713232/93‑1 (MQ=255)
                                                                             gcgcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGtg             >  1:2519232/1‑92 (MQ=255)
                                                                             gcgcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTc             <  2:820533/93‑1 (MQ=255)
                                                                             gcgcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGCACATTTAGTGCTTAAGGTc             >  1:2949378/1‑93 (MQ=255)
                                                                               gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           >  2:3995802/1‑93 (MQ=255)
                                                                               gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           >  2:412129/1‑93 (MQ=255)
                                                                               gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           >  1:4719285/1‑93 (MQ=255)
                                                                               gcATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa           >  2:3301743/1‑93 (MQ=255)
                                                                                cATTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa          <  1:1443284/93‑1 (MQ=255)
                                                                                 ctttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAc         <  2:219853/92‑1 (MQ=255)
                                                                                 aTTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAc         >  1:99735/1‑93 (MQ=255)
                                                                                 aTTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAc         <  2:2586257/93‑1 (MQ=255)
                                                                                 aTTTCTTTCCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAc         >  2:2285241/1‑93 (MQ=255)
                                                                                  tttctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAct        >  2:3898970/1‑93 (MQ=255)
                                                                                    tctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActct      >  2:2938289/1‑93 (MQ=255)
                                                                                    tctttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActct      >  2:3645250/1‑93 (MQ=255)
                                                                                      tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    >  2:4534015/1‑93 (MQ=255)
                                                                                      tttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCttt    <  2:4440194/93‑1 (MQ=255)
                                                                                       ttcCGCAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTa   <  1:2550679/93‑1 (MQ=255)
                                                                                        tcCGCAGCGCAGCGGGAATTGACTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTaa  <  1:1982072/93‑1 (MQ=255)
                                                                                           |                                                                                         
GTAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAA  >  NC_000913/134747‑134927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑