BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,480,081	T→C	S383S (AGT→AGC)	ynbD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,480,081	0	T	C	99.2%	397.8 / ‑5.7	124	S383S (AGT→AGC)	ynbD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (68/55); total (69/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 130 total aligned reads displayed.

GGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGC  >  NC_000913/1479989‑1480169

ggggAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGt                                                                                          <  2:4113245/93‑1 (MQ=255)

    aGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAgcgcgc                                                                                      <  1:2379549/93‑1 (MQ=255)

    aGCTCCGACAGGCCGTGGCGATGCTCGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAgcgcgc                                                                                      >  1:4690877/1‑93 (MQ=255)

     gCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCt                                                                                     <  1:616015/93‑1 (MQ=255)

       tCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtgg                                                                                   >  1:4394745/1‑93 (MQ=255)

       tCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtgg                                                                                   <  1:3454899/93‑1 (MQ=255)

       tCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtgg                                                                                   >  1:2644341/1‑93 (MQ=255)

       tCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtgg                                                                                   >  1:2170224/1‑93 (MQ=255)

        ccGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggt                                                                                  <  1:4616174/93‑1 (MQ=255)

        ccGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggg                                                                                  >  1:3882929/1‑92 (MQ=255)

         cGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtg                                                                                 <  1:4167399/93‑1 (MQ=255)

         cGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtg                                                                                 <  1:927385/93‑1 (MQ=255)

           aCAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggt                                                                               <  2:3685002/93‑1 (MQ=255)

           aCAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggt                                                                               >  2:1315672/1‑93 (MQ=255)

           aCAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggg                                                                               >  2:1315671/1‑92 (MQ=255)

            cAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggtg                                                                              <  2:2029449/93‑1 (MQ=255)

            cAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggtg                                                                              >  2:532601/1‑93 (MQ=255)

             aGGCCGGGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCtggtggtgg                                                                             <  1:3674980/93‑1 (MQ=255)

               gCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCTTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTggcg                                                                           <  1:1138776/93‑1 (MQ=255)

                 cGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTggcggc                                                                         <  2:3520074/93‑1 (MQ=255)

                 cGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCACCGCGCTGGTGGTggcggc                                                                         >  1:4266683/1‑93 (MQ=255)

                   tGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCAt                                                                       >  1:1848709/1‑93 (MQ=255)

                   tGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCAt                                                                       <  1:1811207/93‑1 (MQ=255)

                    ggCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATg                                                                      <  1:925284/93‑1 (MQ=255)

                     gCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATgg                                                                     <  2:2453167/93‑1 (MQ=255)

                       gATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGgtt                                                                   >  2:4130808/1‑93 (MQ=255)

                          gCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGgttgtt                                                                >  1:204802/1‑93 (MQ=255)

                             ggAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTtgttatt                                                             >  2:4043179/1‑92 (MQ=255)

                               aaaCATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTtgttatgtt                                                           >  1:3487063/1‑93 (MQ=255)

                                aaCATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTtgttatgttc                                                          >  1:3243907/1‑92 (MQ=255)

                                aaCATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTtgttatgtta                                                          >  2:2388505/1‑93 (MQ=255)

                                aaCATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTtgttatgtta                                                          >  1:2969089/1‑93 (MQ=255)

                                  cATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACg                                                        <  1:4359707/93‑1 (MQ=255)

                                  cATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACg                                                        <  2:2766830/93‑1 (MQ=255)

                                    ttACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGa                                                      >  1:4591550/1‑93 (MQ=255)

                                     tACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGac                                                     <  1:1078991/93‑1 (MQ=255)

                                      aCGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGaga                                                    >  1:614899/1‑91 (MQ=255)

                                      aCGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGaca                                                    <  2:4472252/93‑1 (MQ=255)

                                      aCGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGaca                                                    <  2:4257619/93‑1 (MQ=255)

                                       cgcgAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGacac                                                   >  2:3653425/1‑93 (MQ=255)

                                        gcgAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACt                                                  >  2:2112581/1‑93 (MQ=255)

                                         cggagagCAAGACAGCGTTCTGGTCCATTGTGCTTTGGAATTATCGCACAGCGCGCTGGTGGTGGCGGCATAGTTGTTATGTTACGGACACTg                                                 <  2:4214483/90‑1 (MQ=255)

                                         cgAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTg                                                 <  2:191732/93‑1 (MQ=255)

                                          gAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGt                                                <  1:4507611/93‑1 (MQ=255)

                                          gAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGt                                                <  1:3012832/93‑1 (MQ=255)

                                          gAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGt                                                <  2:2261138/93‑1 (MQ=255)

                                           aaGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTa                                               >  1:1064948/1‑93 (MQ=255)

                                             gagCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTaca                                             >  1:1683190/1‑91 (MQ=255)

                                             gagCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTaaa                                             >  1:313296/1‑93 (MQ=255)

                                              agCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTaaaa                                            >  2:1966423/1‑93 (MQ=255)

                                              agCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTaaaa                                            >  1:208641/1‑93 (MQ=255)

                                               gCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAAc                                           >  1:3771070/1‑93 (MQ=255)

                                               gCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAAc                                           <  2:77120/93‑1 (MQ=255)

                                                cAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAAcc                                          <  1:1537763/93‑1 (MQ=255)

                                                 aaGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCg                                         >  1:1723961/1‑93 (MQ=255)

                                                 aaGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCg                                         >  1:1176276/1‑93 (MQ=255)

                                                  aGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGt                                        >  2:4068314/1‑93 (MQ=255)

                                                  aGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGt                                        >  1:4587009/1‑93 (MQ=255)

                                                  aGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGt                                        >  2:4068315/1‑93 (MQ=255)

                                                    gCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAAACGATa                                      >  1:2307449/1‑93 (MQ=255)

                                                     cAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTaa                                     <  2:2658629/93‑1 (MQ=255)

                                                       gCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATg                                   <  2:769675/93‑1 (MQ=255)

                                                       gCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATg                                   >  2:3706148/1‑93 (MQ=255)

                                                        cGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGa                                  >  1:1774471/1‑93 (MQ=255)

                                                        cGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGa                                  <  2:3953082/93‑1 (MQ=255)

                                                          ttCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGAGGTGGCGGCATGGTTGTTATGTTACGGACCCTGTAAAACGGTTAATGAAg                                >  2:2332493/1‑93 (MQ=255)

                                                          ttCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAg                                >  2:2388406/1‑93 (MQ=255)

                                                           tCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGc                               <  2:3387433/93‑1 (MQ=255)

                                                           tCTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGc                               >  1:4091774/1‑93 (MQ=255)

                                                            cTGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCg                              <  1:3634670/93‑1 (MQ=255)

                                                             tGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGa                             <  1:1680714/93‑1 (MQ=255)

                                                             tGGTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGa                             <  2:3083858/93‑1 (MQ=255)

                                                              ggTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGAt                            >  2:739379/1‑93 (MQ=255)

                                                              ggTCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGAt                            <  2:1492506/93‑1 (MQ=255)

                                                                tCCATTGTGCTTTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGGTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTa                          >  2:4195350/1‑93 (MQ=255)

                                                                tCCATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTa                          <  2:2146876/93‑1 (MQ=255)

                                                                gccATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTa                          <  1:1999789/92‑1 (MQ=255)

                                                                 ccATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAg                         <  2:457172/93‑1 (MQ=255)

                                                                 ccATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAg                         >  2:117673/1‑93 (MQ=255)

                                                                  cATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGCAGCGATTAGc                        >  1:2473482/1‑93 (MQ=255)

                                                                  cATTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGc                        >  2:2724139/1‑93 (MQ=255)

                                                                   aTTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCt                       >  1:3193359/1‑93 (MQ=255)

                                                                   aTTGTGCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCt                       >  2:460888/1‑93 (MQ=255)

                                                                     tgtgCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCtat                     <  2:1630411/93‑1 (MQ=255)

                                                                       tgCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTatat                   <  2:816456/93‑1 (MQ=255)

                                                                        gCATTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATAtt                  >  2:313951/1‑93 (MQ=255)

                                                                          aTTGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCg                >  1:3573742/1‑93 (MQ=255)

                                                                           ttGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGa               >  1:836669/1‑93 (MQ=255)

                                                                           ttGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGa               <  2:669035/93‑1 (MQ=255)

                                                                           ttGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGa               <  2:669176/93‑1 (MQ=255)

                                                                            tGGGATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAg              >  2:517979/1‑93 (MQ=255)

                                                                             gggATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGc             >  2:2148910/1‑93 (MQ=255)

                                                                             gggATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGc             <  2:3254045/93‑1 (MQ=255)

                                                                              ggATTATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGcc            >  1:536248/1‑93 (MQ=255)

                                                                                 ttATCGCGCAGCACGATGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGa         <  2:4220305/93‑1 (MQ=255)

                                                                                  tATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGAc        <  2:1336881/93‑1 (MQ=255)

                                                                                  tATCGCGCAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGAc        <  1:122766/93‑1 (MQ=255)

                                                                                      gcgcAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGccc    >  1:2812683/1‑93 (MQ=255)

                                                                                       cgcAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCg   <  2:4261922/93‑1 (MQ=255)

                                                                                       cgcAGCGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCg   >  1:1058253/1‑93 (MQ=255)

GGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGC  >  NC_000913/1479989‑1480169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`