BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,482,154	T→C	V357A (GTA→GCA)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,154	0	T	C	96.0%	363.3 / 4.2	126	V357A (GTA→GCA)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (55/66); total (57/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 131 total aligned reads displayed.

TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGC  >  NC_000913/1482064‑1482243

tttCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAc                                                                                         >  1:3591862/1‑93 (MQ=255)

    caaCAGCGAACAAAGTAGGTTATTCCAGTCGCACAGCGGACGGCGTATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCggg                                                                                     <  2:4411327/92‑1 (MQ=255)

     aaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgggg                                                                                    <  2:4212630/93‑1 (MQ=255)

      aCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCggggt                                                                                   >  1:3945674/1‑92 (MQ=255)

      aCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGa                                                                                   <  1:3834595/93‑1 (MQ=255)

      aCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGa                                                                                   >  2:4228499/1‑93 (MQ=255)

        aGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGAtt                                                                                 <  1:4096753/93‑1 (MQ=255)

         gCGAACAAAATAGGGAATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTa                                                                                >  2:3893590/1‑93 (MQ=255)

          cGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGTGATTaa                                                                               <  1:1701706/93‑1 (MQ=255)

          cGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTaa                                                                               <  1:1701707/93‑1 (MQ=255)

           gAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTaaa                                                                              >  1:1633691/1‑93 (MQ=255)

            aaCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAAt                                                                             <  1:2323127/93‑1 (MQ=255)

             aCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATa                                                                            <  2:4026514/93‑1 (MQ=255)

             aCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATa                                                                            >  2:3435209/1‑93 (MQ=255)

             aCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATa                                                                            >  2:3404513/1‑93 (MQ=255)

               aaaaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACg                                                                          <  1:131678/93‑1 (MQ=255)

                aaaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGt                                                                         >  2:797675/1‑93 (MQ=255)

                 aaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGtt                                                                        <  1:3432743/93‑1 (MQ=255)

                 aaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGtt                                                                        <  1:1321375/93‑1 (MQ=255)

                  aTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGCCCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTa                                                                       <  2:3817112/93‑1 (MQ=255)

                  aTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTa                                                                       <  2:2723953/93‑1 (MQ=255)

                   tAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAt                                                                      <  1:1058392/93‑1 (MQ=255)

                    aGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATc                                                                     <  1:2611789/93‑1 (MQ=255)

                    aGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATc                                                                     <  1:763423/93‑1 (MQ=255)

                      ggTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAACGGTGCCGAACCCAACCGGGG‑TTAAATACGGTAttcac                                                                  >  1:1925172/1‑89 (MQ=255)

                      ggTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGa                                                                   >  2:4378452/1‑93 (MQ=255)

                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcc                                                                 >  2:190545/1‑93 (MQ=255)

                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcc                                                                 <  1:430964/93‑1 (MQ=255)

                         aTTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCTACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAccc                                                                <  1:3075776/93‑1 (MQ=255)

                         aTTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAccc                                                                >  2:3443766/1‑93 (MQ=255)

                          ttCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcccc                                                               <  2:1835012/93‑1 (MQ=255)

                          ttCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcccc                                                               <  1:47097/93‑1 (MQ=255)

                          ttCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcccc                                                               <  2:3642238/93‑1 (MQ=255)

                            ccAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCgg                                                             <  1:2678739/93‑1 (MQ=255)

                            ccAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCgg                                                             >  2:3453805/1‑93 (MQ=255)

                             cAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCggc                                                            >  1:1072357/1‑92 (MQ=255)

                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCTACCCCgggag                                                          >  2:1574076/1‑90 (MQ=255)

                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAc                                                          >  1:2153597/1‑93 (MQ=255)

                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAc                                                          <  1:3208282/93‑1 (MQ=255)

                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGGAc                                                          >  2:1085341/1‑93 (MQ=255)

                                tCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACa                                                         <  2:1235599/93‑1 (MQ=255)

                                  gCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTCCAgc                                                       >  1:419206/1‑93 (MQ=255)

                                  gCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgc                                                       <  1:452160/93‑1 (MQ=255)

                                  gCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGGACAgc                                                       >  2:2886654/1‑93 (MQ=255)

                                   cacaGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgcg                                                      >  1:2834878/1‑93 (MQ=255)

                                   cacaGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgcg                                                      >  1:2834877/1‑93 (MQ=255)

                                    acaGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgcgc                                                     >  1:3664724/1‑93 (MQ=255)

                                     caGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGcgcg                                                    >  1:865951/1‑93 (MQ=255)

                                     caGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGcgcg                                                    <  1:470476/93‑1 (MQ=255)

                                      aGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGt                                                   >  1:829775/1‑93 (MQ=255)

                                         ggACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATc                                                <  2:2787165/93‑1 (MQ=255)

                                         ggACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATc                                                >  2:2874886/1‑93 (MQ=255)

                                         ggACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATc                                                <  1:3713978/93‑1 (MQ=255)

                                              gcgcATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCg                                           >  1:1831089/1‑93 (MQ=255)

                                               cgcATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGc                                          <  1:3511544/93‑1 (MQ=255)

                                                 cATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTa                                        >  1:2390674/1‑93 (MQ=255)

                                                 cATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTa                                        <  2:2280939/93‑1 (MQ=255)

                                                  aTTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAc                                       >  1:2871197/1‑93 (MQ=255)

                                                   ttGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACa                                      >  2:4663625/1‑93 (MQ=255)

                                                   ttGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACa                                      <  1:1227704/93‑1 (MQ=255)

                                                     gtgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGc                                    <  2:2756993/93‑1 (MQ=255)

                                                      tgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCt                                   <  1:3805211/93‑1 (MQ=255)

                                                      tgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCt                                   <  1:2578103/93‑1 (MQ=255)

                                                       gCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTa                                  <  2:850517/93‑1 (MQ=255)

                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTGCAGCCATc                                >  1:781756/1‑93 (MQ=255)

                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                                >  1:781755/1‑93 (MQ=255)

                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                                <  1:3430191/93‑1 (MQ=255)

                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                                >  1:4048452/1‑93 (MQ=255)

                                                          ggCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCg                               >  2:536315/1‑93 (MQ=255)

                                                            cGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCa                             <  1:3775700/93‑1 (MQ=255)

                                                             gACCAACGTCGCGGAAACGTCGCTGACTTCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAc                            <  1:4729148/93‑1 (MQ=255)

                                                             gACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGACGCTACAGCTATCGCAc                            >  1:2484062/1‑93 (MQ=255)

                                                              aCCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAcc                           >  1:1657122/1‑93 (MQ=255)

                                                              aCCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAcc                           <  2:1453201/93‑1 (MQ=255)

                                                              aCCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAcc                           <  1:1905465/93‑1 (MQ=255)

                                                                cAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaa                         <  2:4001421/93‑1 (MQ=255)

                                                                cAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaa                         <  1:1075720/93‑1 (MQ=255)

                                                                cAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaa                         >  1:481786/1‑93 (MQ=255)

                                                                cAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaa                         <  1:2840079/93‑1 (MQ=255)

                                                                 aaCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaaa                        >  1:20257/1‑93 (MQ=255)

                                                                 aaCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCACGTATCAGCCGCTACAGCTATCGCACCaaa                        >  2:4638208/1‑93 (MQ=255)

                                                                   cGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGt                      <  2:2076544/93‑1 (MQ=255)

                                                                   cGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGt                      <  2:4267564/93‑1 (MQ=255)

                                                                     tCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGc                    >  2:2332258/1‑93 (MQ=255)

                                                                     tCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGc                    >  2:2887890/1‑93 (MQ=255)

                                                                      cgcgGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCa                   <  2:3045288/93‑1 (MQ=255)

                                                                       gcgGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAg                  >  1:4328454/1‑93 (MQ=255)

                                                                       gcgGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAg                  >  2:3910181/1‑93 (MQ=255)

                                                                         ggAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCg                >  2:2158452/1‑93 (MQ=255)

                                                                          gAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGt               >  1:1559579/1‑93 (MQ=255)

                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt              <  1:1911826/93‑1 (MQ=255)

                                                                            aaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGttt             >  2:2479595/1‑93 (MQ=255)

                                                                            aaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGttt             <  2:806022/93‑1 (MQ=255)

                                                                                 cGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGa        <  2:2564294/93‑1 (MQ=255)

                                                                                   cTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGAtt      <  1:1355712/93‑1 (MQ=255)

                                                                                   cTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGAtt      <  1:781344/93‑1 (MQ=255)

                                                                                   cTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGAtt      >  2:1028117/1‑93 (MQ=255)

                                                                                     gACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGa    >  1:300316/1‑93 (MQ=255)

                                                                                       ccGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGc  <  1:2402674/93‑1 (MQ=255)

                                                                                       ccGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGc  >  1:1543913/1‑93 (MQ=255)

TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGC  >  NC_000913/1482064‑1482243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`