BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,484,846	A→G	A1254A (GCA→GCG)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,484,846	0	A	G	97.2%	433.3 / 1.6	143	A1254A (GCA→GCG)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (65/74); total (66/77)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

Only 100 of 151 total aligned reads displayed.

GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935

ggTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCcgcgcg                                                                                         >  2:3676229/1‑93 (MQ=255)

 gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                        >  1:4574835/1‑93 (MQ=255)

 gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                        <  1:175464/93‑1 (MQ=255)

  ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                       <  2:2438552/93‑1 (MQ=255)

  ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                       <  2:4745153/93‑1 (MQ=255)

   tGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcg                                                                                      >  2:2176560/1‑93 (MQ=255)

    gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     <  1:4463719/93‑1 (MQ=255)

    gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     >  1:2567259/1‑93 (MQ=255)

     aTCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTg                                                                                    >  1:1266393/1‑93 (MQ=255)

      tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   <  2:116066/93‑1 (MQ=255)

      tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   >  2:3775977/1‑93 (MQ=255)

          caTCGCGACAGTGCGCAGATGCTGAAAGTAGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAt                                                                               >  2:416169/1‑93 (MQ=255)

           aTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATg                                                                              >  2:4137427/1‑93 (MQ=255)

             cgcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGaa                                                                            <  1:1009876/93‑1 (MQ=255)

              gcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAg                                                                           <  2:225039/93‑1 (MQ=255)

              gcgACCGTGCGCAGATGCTGAAAGTCAAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGgaagaat                                                                           <  2:225041/93‑2 (MQ=255)

               cgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGa                                                                          <  2:3531138/93‑1 (MQ=255)

                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        >  1:1878169/1‑93 (MQ=255)

                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        >  1:239340/1‑93 (MQ=255)

                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        >  2:320823/1‑93 (MQ=255)

                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        >  1:4271068/1‑93 (MQ=255)

                    gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     >  2:4065159/1‑93 (MQ=255)

                      gcgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGaaa                                                                   <  1:888876/93‑1 (MQ=255)

                       cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  >  1:3509425/1‑93 (MQ=255)

                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 <  1:162057/93‑1 (MQ=255)

                         cAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAagag                                                                <  1:3011026/93‑1 (MQ=255)

                           gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGGTCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              >  1:544962/1‑93 (MQ=255)

                           gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgagac                                                              >  1:3384499/1‑92 (MQ=255)

                           gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              <  1:32230/93‑1 (MQ=255)

                           gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              <  2:4012513/93‑1 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  1:3949537/93‑1 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            >  2:1100638/1‑93 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  1:3111218/93‑1 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            >  1:2376236/1‑93 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            >  1:2376235/1‑93 (MQ=255)

                                tGAAATTCAAAAACGTCCAGCAGGCGTGACAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         <  1:2040011/93‑1 (MQ=255)

                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  2:4503715/1‑93 (MQ=255)

                                  aaaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCGGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTgg                                                       <  1:2563169/93‑1 (MQ=255)

                                  aaaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTgg                                                       >  2:4419038/1‑93 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      >  1:3298579/1‑93 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      <  2:3616425/93‑1 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      >  2:2842836/1‑93 (MQ=255)

                                    aGTCGAAAACGTCCAGGAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgat                                                     >  2:2309638/1‑93 (MQ=255)

                                      tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   >  2:2787298/1‑93 (MQ=255)

                                       cGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatgat                                                  <  1:4303679/93‑1 (MQ=255)

                                       cGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatgat                                                  <  2:1956170/93‑1 (MQ=255)

                                         aaaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATag                                                >  2:3283333/1‑93 (MQ=255)

                                           aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              >  1:2309139/1‑93 (MQ=255)

                                           aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              <  2:815382/93‑1 (MQ=255)

                                             cGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                            <  1:1188385/93‑1 (MQ=255)

                                             cGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                            >  2:2565495/1‑93 (MQ=255)

                                             cGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCTGCCCGCGTGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                            <  1:19091/93‑1 (MQ=255)

                                              gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                           <  1:4460144/93‑1 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          >  1:2325493/1‑93 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          <  1:3832972/93‑1 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          <  1:2693019/93‑1 (MQ=255)

                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  2:1834475/93‑1 (MQ=255)

                                                  cccagGCGTGGCAGCAATGGATCAACATACGCACGCTCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:2348364/91‑1 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGTATGATAGAAGAGTTgc                                       >  1:2114832/1‑93 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:2667902/93‑1 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:2379285/93‑1 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:2675208/93‑1 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       >  1:1025934/1‑93 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  1:35390/93‑1 (MQ=255)

                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   <  1:3635093/93‑1 (MQ=255)

                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   >  2:810163/1‑93 (MQ=255)

                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGGTGCGCgt                                   >  2:1261954/1‑93 (MQ=255)

                                                       gCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtt                                  >  1:3287313/1‑93 (MQ=255)

                                                         gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttag                                >  2:2163382/1‑93 (MQ=255)

                                                         gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttag                                <  2:2794625/93‑1 (MQ=255)

                                                           ggCAGCAATGGATCAACAAACTGTCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              <  2:3386074/93‑1 (MQ=255)

                                                             gagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                            <  1:613691/92‑1 (MQ=255)

                                                             cagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                            >  1:796342/1‑93 (MQ=255)

                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           >  2:2565353/1‑93 (MQ=255)

                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           <  1:2797086/93‑1 (MQ=255)

                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           <  2:3408388/93‑1 (MQ=255)

                                                               gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                          <  2:4715588/93‑1 (MQ=255)

                                                               gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                          <  2:1954234/93‑1 (MQ=255)

                                                                caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                         <  1:2444468/93‑1 (MQ=255)

                                                                caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                         >  1:1562347/1‑93 (MQ=255)

                                                                 aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                        >  1:2185669/1‑93 (MQ=255)

                                                                 aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGCATGATAGAAGAGTTGCGCGTTAGTTACTTCg                        >  1:2073550/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  2:942137/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  1:4077649/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  2:1748930/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  1:4077648/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      <  1:4380808/93‑1 (MQ=255)

                                                                        caacaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaaca                 <  2:4370686/93‑1 (MQ=255)

                                                                          acaaCCTGCCGCCCGCGCGTCGTGAGGAGGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  2:4599647/93‑1 (MQ=255)

                                                                            aaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtt             >  2:337675/1‑93 (MQ=255)

                                                                            aaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtt             <  2:3743610/93‑1 (MQ=255)

                                                                               cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          <  1:3189046/93‑1 (MQ=255)

                                                                                 gccgccCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAc        <  1:79424/93‑1 (MQ=255)

                                                                                   cgcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGc      <  2:1347823/93‑1 (MQ=255)

                                                                                    gcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGcc     >  2:2028277/1‑93 (MQ=255)

                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  1:517601/1‑93 (MQ=255)

                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  2:2055932/1‑93 (MQ=255)

                                                                                      ccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCtt   <  1:4447601/93‑1 (MQ=255)

                                                                                       cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  <  2:2877396/93‑1 (MQ=255)

                                                                                       cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  <  1:1710912/93‑1 (MQ=255)

GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`