BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,599	T→C	H292R (CAC→CGC)	b1428 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,599	0	T	C	97.7%	396.9 / ‑0.4	129	H292R (CAC→CGC)	b1428	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (60/66); total (62/67)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

Only 100 of 141 total aligned reads displayed.

GCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497510‑1497688

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCggc                                                                                        >  1:1494365/1‑92 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:1494366/1‑93 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:268366/1‑93 (MQ=255)

 gcgcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTc                                                                                       <  1:1752426/91‑1 (MQ=255)

 cggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTc                                                                                       >  2:2585488/1‑93 (MQ=255)

 cggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTc                                                                                       <  2:1715300/93‑1 (MQ=255)

 cggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTc                                                                                       <  1:2853886/93‑1 (MQ=255)

  ggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCg                                                                                      <  2:2937798/93‑1 (MQ=255)

  ggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCg                                                                                      >  1:3924964/1‑93 (MQ=255)

  ggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCg                                                                                      >  1:3368360/1‑93 (MQ=255)

   gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     >  2:3879250/1‑93 (MQ=255)

   gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     <  1:726774/93‑1 (MQ=255)

    cgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGt                                                                                    <  2:4530291/93‑1 (MQ=255)

      ttCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTa                                                                                  <  1:1876792/93‑1 (MQ=255)

      ttCGCGTAATGCGATCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTa                                                                                  <  1:1084778/93‑1 (MQ=255)

        cgcgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAAc                                                                                <  1:940281/93‑1 (MQ=255)

         gcgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGGTAACt                                                                               >  1:1751172/1‑93 (MQ=255)

            tAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCca                                                                            <  1:2170826/93‑1 (MQ=255)

            tAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCca                                                                            >  1:3012375/1‑93 (MQ=255)

            tAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAAAGACGGCGCCGTCGGTTAACTCca                                                                            >  2:3196332/1‑93 (MQ=255)

             aaTGCGCTCCTCGCCGTTAATGACTTTTGGACCACGCACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCcac                                                                           <  2:1770583/93‑1 (MQ=255)

              aTGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCcaca                                                                          <  2:4675612/93‑1 (MQ=255)

                 cgcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCATAGGCTTCAACGACGGCGCGGTCGGGTAACTCCACATgg                                                                       >  2:2648472/1‑93 (MQ=255)

                 cgcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATgg                                                                       <  2:848670/93‑1 (MQ=255)

                  gcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGt                                                                      >  1:2117971/1‑93 (MQ=255)

                   ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  2:2450478/93‑1 (MQ=255)

                   ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGGTTACTCCACATGGtt                                                                     >  2:4296622/1‑93 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  1:1288604/1‑93 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  2:231610/1‑93 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  1:2418651/1‑93 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtgt                                                                    >  1:369343/1‑91 (MQ=255)

                     cctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtttt                                                                   <  1:3542259/93‑1 (MQ=255)

                      ctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTc                                                                  <  2:2710426/93‑1 (MQ=255)

                      ctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTc                                                                  <  2:4542162/93‑1 (MQ=255)

                       tcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCa                                                                 <  1:4663126/93‑1 (MQ=255)

                        cGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCaa                                                                <  1:3229715/93‑1 (MQ=255)

                         gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAt                                                               <  2:1190064/93‑1 (MQ=255)

                         gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAt                                                               >  2:4342260/1‑93 (MQ=255)

                         gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAt                                                               <  1:2881280/93‑1 (MQ=255)

                          ccGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAtt                                                              >  1:706173/1‑93 (MQ=255)

                           cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             >  1:128594/1‑93 (MQ=255)

                           cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             >  2:1403544/1‑93 (MQ=255)

                            gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  2:2177085/1‑93 (MQ=255)

                            gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            <  1:463239/93‑1 (MQ=255)

                            gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  2:641305/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:2174751/93‑1 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  1:1436116/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:1441876/93‑1 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:311606/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          >  2:1304994/1‑93 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         >  2:887630/1‑93 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         <  1:1938728/93‑1 (MQ=255)

                                 tGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCg                                                       >  2:2516485/1‑93 (MQ=255)

                                 tGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCg                                                       <  2:1908527/93‑1 (MQ=255)

                                      tttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCt                                                  >  2:3514032/1‑93 (MQ=255)

                                      tttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCt                                                  >  1:2952175/1‑93 (MQ=255)

                                      tttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCt                                                  >  2:1978531/1‑93 (MQ=255)

                                       ttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTg                                                 >  2:4635893/1‑93 (MQ=255)

                                        tGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGt                                                <  1:1437761/93‑1 (MQ=255)

                                          gACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTaa                                              <  1:3991696/93‑1 (MQ=255)

                                          gACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGAGGGCGGATAGCTCCACATGGTTTTCAATTATCACCGCACCTGTaa                                              >  1:697456/1‑93 (MQ=255)

                                            ccACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATa                                            >  2:23406/1‑93 (MQ=255)

                                             cACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATAc                                           <  2:564458/93‑1 (MQ=255)

                                             cACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATAc                                           >  1:414837/1‑93 (MQ=255)

                                              aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACg                                          >  2:2874327/1‑93 (MQ=255)

                                              aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACg                                          >  2:1625655/1‑93 (MQ=255)

                                                ggACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGc                                        >  1:3095440/1‑93 (MQ=255)

                                                      gAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCAc                                  >  2:1318809/1‑93 (MQ=255)

                                                       aaCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCAcc                                 <  2:4689631/93‑1 (MQ=255)

                                                        aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                >  1:2008653/1‑93 (MQ=255)

                                                         ccGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCtt                               >  2:2705012/1‑93 (MQ=255)

                                                         ccGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCtt                               <  1:2717780/93‑1 (MQ=255)

                                                          cgcgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTg                              <  2:2328477/90‑1 (MQ=255)

                                                           gtgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGt                             >  1:4162471/1‑93 (MQ=255)

                                                              tCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATg                          >  2:576221/1‑93 (MQ=255)

                                                                cccATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGAc                        <  2:2705900/92‑1 (MQ=255)

                                                                 ccATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACa                       <  2:1895863/93‑1 (MQ=255)

                                                                  cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACac                      >  1:945459/1‑92 (MQ=255)

                                                                  cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      <  1:3229308/93‑1 (MQ=255)

                                                                    tCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACg                    <  2:1601134/93‑1 (MQ=255)

                                                                    tCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACg                    >  2:516266/1‑93 (MQ=255)

                                                                       aaGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATAACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGc                 <  1:4706743/93‑1 (MQ=255)

                                                                        aGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGTTCTCACCTTGTATGACAACGTggt                >  1:1127737/1‑91 (MQ=255)

                                                                        aGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCt                <  2:1317819/93‑1 (MQ=255)

                                                                           cTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgt             <  2:1986510/93‑1 (MQ=255)

                                                                            ttCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtc            <  1:3440879/93‑1 (MQ=255)

                                                                            ttCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtc            <  1:2387467/93‑1 (MQ=255)

                                                                            ttCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtc            <  2:1547405/93‑1 (MQ=255)

                                                                             tCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCCCCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtcg           <  2:1757502/93‑1 (MQ=255)

                                                                              cAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGa          <  1:3328952/93‑1 (MQ=255)

                                                                                acgacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGc        >  2:1281882/1‑93 (MQ=255)

                                                                                   acgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGa     <  1:3280191/93‑1 (MQ=255)

                                                                                    cgGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGaa    >  2:3124916/1‑93 (MQ=255)

                                                                                    cgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGaa    <  2:771892/93‑1 (MQ=255)

                                                                                    cgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGaa    <  1:1896384/93‑1 (MQ=255)

                                                                                    cgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGaa    <  2:3908738/93‑1 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  1:1042184/93‑1 (MQ=255)

                                                                                      gcgcGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc  <  1:3850301/93‑1 (MQ=255)

                                                                                      gcgcGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc  <  1:3850302/93‑1 (MQ=255)

                                                                                      gcgcGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc  <  1:1658192/93‑1 (MQ=255)

GCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497510‑1497688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`