BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,498,686	C→T	A30A (GCC→GCT)	tehA →	TehA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,498,686	0	C	T	98.0%	345.2 / ‑3.3	101	A30A (GCC→GCT)	tehA	TehA
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (56/43); total (58/43)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 120 total aligned reads displayed.

AGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAT  >  NC_000913/1498595‑1498775

agaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgcca                                                                                          >  1:4275929/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGGTAGTGATGGGATTGTCCTGGCGCTATgcgag                                                                                         >  1:503146/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctag                                                                                         >  1:2599246/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctag                                                                                         >  1:92526/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctag                                                                                         >  1:4163806/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctag                                                                                         >  1:1615600/1‑93 (MQ=255)

 gaTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACATTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGTCTGGCGCTATgctag                                                                                         >  2:315274/1‑93 (MQ=255)

  aTGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagc                                                                                        <  2:3478866/93‑1 (MQ=255)

   tGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagcc                                                                                       >  2:1797387/1‑93 (MQ=255)

   tGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagcc                                                                                       >  2:868523/1‑93 (MQ=255)

   tGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagcc                                                                                       >  1:2083965/1‑93 (MQ=255)

    gCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagcca                                                                                      >  1:1899092/1‑93 (MQ=255)

    gCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgctagcca                                                                                      >  1:766374/1‑93 (MQ=255)

       gagCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGt                                                                                   <  2:884333/93‑1 (MQ=255)

       gagCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGt                                                                                   >  2:1529958/1‑93 (MQ=255)

         gCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGttt                                                                                 >  1:3356451/1‑93 (MQ=255)

         gCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGttt                                                                                 <  1:3701875/93‑1 (MQ=255)

         gCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGttt                                                                                 >  1:1363722/1‑93 (MQ=255)

         gCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGttt                                                                                 >  1:1363723/1‑93 (MQ=255)

         gCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGGATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGttt                                                                                 >  1:2381988/1‑93 (MQ=255)

           gATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTgg                                                                               <  2:1478419/93‑1 (MQ=255)

            aTAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGc                                                                              <  1:1012868/93‑1 (MQ=255)

             tAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCa                                                                             >  2:2441190/1‑93 (MQ=255)

              aaaGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAg                                                                            <  2:239887/93‑1 (MQ=255)

                aGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGt                                                                          <  1:4693574/93‑1 (MQ=255)

                aGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGt                                                                          <  2:3611342/93‑1 (MQ=255)

                 gTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGta                                                                         >  1:244351/1‑92 (MQ=255)

                 gTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTc                                                                         >  1:1907994/1‑93 (MQ=255)

                 gTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTc                                                                         >  1:342107/1‑93 (MQ=255)

                  tGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCa                                                                        >  2:4532281/1‑93 (MQ=255)

                  tGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCa                                                                        >  2:2254663/1‑93 (MQ=255)

                     tCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGcc                                                                     <  2:2551019/93‑1 (MQ=255)

                       aaTTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCAc                                                                   <  2:2083049/93‑1 (MQ=255)

                         tttGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTg                                                                 <  1:911590/93‑1 (MQ=255)

                         tttGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTg                                                                 >  1:1285713/1‑93 (MQ=255)

                           tGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGt                                                               >  2:4541342/1‑93 (MQ=255)

                             ccGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTa                                                             <  1:1298396/93‑1 (MQ=255)

                             ccGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTa                                                             <  1:4523588/93‑1 (MQ=255)

                              cGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAg                                                            >  1:3201112/1‑93 (MQ=255)

                               ggCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAgg                                                           >  2:4572793/1‑93 (MQ=255)

                               ggCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGGTAgg                                                           >  2:3251070/1‑93 (MQ=255)

                                   ggCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGAt                                                       >  2:2791431/1‑93 (MQ=255)

                                    gCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATg                                                      >  2:4409457/1‑93 (MQ=255)

                                     cTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATgg                                                     <  2:1040067/93‑1 (MQ=255)

                                       aCTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGc                                                   >  1:3488449/1‑93 (MQ=255)

                                       aCTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGc                                                   >  2:1778597/1‑93 (MQ=255)

                                        cTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCt                                                  >  1:2100358/1‑93 (MQ=255)

                                         tttGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGGTGGGCTg                                                 >  2:3831441/1‑93 (MQ=255)

                                           tGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGt                                               <  2:3631950/93‑1 (MQ=255)

                                             gTATTGTGTTGGGGACGCTAGGGCTGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGGCACCCACTGCTTCGGGGCGGGGCTGGTgc                                             >  1:210126/1‑92 (MQ=255)

                                             gTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGa                                             >  1:210127/1‑93 (MQ=255)

                                              tATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAt                                            <  2:2178991/93‑1 (MQ=255)

                                               aTTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAtt                                           <  1:2106836/93‑1 (MQ=255)

                                               aTTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAtt                                           <  1:2399931/93‑1 (MQ=255)

                                                ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGAttt                                          >  1:1221830/1‑92 (MQ=255)

                                                ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                          <  2:3676699/93‑1 (MQ=255)

                                                ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                          <  2:4574290/93‑1 (MQ=255)

                                                ttGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTc                                          <  2:4566027/93‑1 (MQ=255)

                                                     ttGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGGTTCTGGCg                                     >  2:1800256/1‑93 (MQ=255)

                                                        gggACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatg                                  <  2:3488222/93‑1 (MQ=255)

                                                        gggACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatg                                  <  2:1206275/93‑1 (MQ=255)

                                                         ggACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatga                                 <  1:316735/93‑1 (MQ=255)

                                                          gACGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCgatgat                                >  1:496067/1‑93 (MQ=255)

                                                           gcGATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatc                               <  2:2730002/92‑1 (MQ=255)

                                                             gATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatcat                             >  2:4113750/1‑93 (MQ=255)

                                                             gATAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatcat                             >  2:4398718/1‑93 (MQ=255)

                                                              aTAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGatcatc                            >  1:2851281/1‑93 (MQ=255)

                                                               tAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCt                           <  2:809774/93‑1 (MQ=255)

                                                               tAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCt                           <  1:665688/93‑1 (MQ=255)

                                                               tAGGGATGGGATTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCt                           >  1:1348467/1‑93 (MQ=255)

                                                                 gggatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTgg                         >  2:759517/1‑93 (MQ=255)

                                                                  ggatgggatTTGCCTGGCTCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTggg                        <  2:1958558/93‑1 (MQ=255)

                                                                  ggatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGATAGGGGATGGGCTGGTTATTCTGGCGATTATGATCTggg                        >  2:2464069/1‑93 (MQ=255)

                                                                   gatgggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTgggg                       <  2:1396404/93‑1 (MQ=255)

                                                                      gggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatt                    >  1:3085874/1‑93 (MQ=255)

                                                                      gggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatt                    >  2:1381571/1‑93 (MQ=255)

                                                                      gggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatt                    <  2:55307/93‑1 (MQ=255)

                                                                      gggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatt                    >  2:1381570/1‑93 (MQ=255)

                                                                      gggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatt                    <  2:1242399/93‑1 (MQ=255)

                                                                       ggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatta                   >  1:4236704/1‑93 (MQ=255)

                                                                       ggatTTGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGatta                   <  1:4208472/93‑1 (MQ=255)

                                                                         atTTGCCTGGCGCTATGCTAGCCAGGTTTGGTAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGattatt                 >  2:2935859/1‑93 (MQ=255)

                                                                          tttGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTg                >  1:2213355/1‑93 (MQ=255)

                                                                           ttGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGa               >  1:2278679/1‑93 (MQ=255)

                                                                            tGCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGAc              >  1:469178/1‑93 (MQ=255)

                                                                             gCCTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACt             >  2:1989551/1‑93 (MQ=255)

                                                                              ccTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTa            <  2:2090718/93‑1 (MQ=255)

                                                                               cTGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAg           >  2:3503640/1‑93 (MQ=255)

                                                                                tGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgc          >  1:3280803/1‑93 (MQ=255)

                                                                                tGGCGCTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgc          >  1:2582039/1‑93 (MQ=255)

                                                                                 ggCGCTATGCCAGCCAGGTTTGGCAGTTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgcg         <  2:2461314/93‑1 (MQ=255)

                                                                                  gcgcTATTCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAgcgc        <  2:2490794/93‑1 (MQ=255)

                                                                                    gcTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAt      <  2:2212244/93‑1 (MQ=255)

                                                                                     cTATGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCAtt     >  1:873044/1‑93 (MQ=255)

                                                                                       aTGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTa   <  2:271032/93‑1 (MQ=255)

                                                                                        tGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGGTGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAt  >  2:1126189/1‑93 (MQ=255)

                                                                                        tGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGGTTATTGACTAGCGCATTTAt  >  2:1249165/1‑93 (MQ=255)

                                                                                        tGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAt  <  2:3098572/93‑1 (MQ=255)

                                                                                        tGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAt  <  2:2979164/93‑1 (MQ=255)

                                                                                        tGCTAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAt  <  2:2979166/93‑1 (MQ=255)

AGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTAGGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTAT  >  NC_000913/1498595‑1498775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`