Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,599,943 G→A A144T (GCC→ACC)  b1513 → putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,599,9430GA97.6% 415.5 / ‑2.1 127A144T (GCC→ACC) b1513putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (62/62);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 131 total aligned reads displayed.

AACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCC  >  NC_000913/1599854‑1600031
                                                                                         |                                                                                        
aaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCg                                                                                       >  1:4264505/1‑93 (MQ=255)
aaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCg                                                                                       >  1:2547268/1‑93 (MQ=255)
 aCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGa                                                                                      >  2:3338198/1‑93 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:2953755/93‑1 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  1:1681253/93‑1 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:45588/93‑1 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  1:4270127/93‑1 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                     <  2:45589/93‑1 (MQ=255)
  cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCACTGGATGTCACCGAt                                                                                     <  1:1681252/93‑1 (MQ=255)
   aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                    <  1:1328738/93‑1 (MQ=255)
   aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                    <  1:4504213/93‑1 (MQ=255)
   aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                    <  1:4257693/93‑1 (MQ=255)
   aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                    <  2:617395/93‑1 (MQ=255)
      tctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGcc                                                                                 <  1:1218039/93‑1 (MQ=255)
         ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                              <  2:4027279/93‑1 (MQ=255)
            tGCAGAAAATTAAGAACCTGCTGGCGGCGCTGGGCTGCCAGCTTGATCTGCATAGTCTGGCAGGATCGCTGGGTGGCACCGATCGACAAAtgg                                                                           >  2:570842/1‑93 (MQ=255)
            tGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtgg                                                                           <  2:4393574/93‑1 (MQ=255)
             gCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtggt                                                                          <  1:3994868/93‑1 (MQ=255)
               aGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtggtgg                                                                        >  1:3088484/1‑93 (MQ=255)
                gAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGa                                                                       >  2:73497/1‑93 (MQ=255)
                  aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                     <  2:4401552/93‑1 (MQ=255)
                  aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                     <  1:2876505/93‑1 (MQ=255)
                   aaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCATGATCGCTGGCAGTCACCGATCGCCAAATGGAAGAAAt                                                                    >  1:3231676/1‑93 (MQ=255)
                   aaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAt                                                                    >  1:2387523/1‑93 (MQ=255)
                   aaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAt                                                                    >  2:524847/1‑93 (MQ=255)
                    aTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtc                                                                   >  2:2061889/1‑93 (MQ=255)
                     tGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTTCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  >  1:1682184/1‑93 (MQ=255)
                     tGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  <  1:2699334/93‑1 (MQ=255)
                     tGAAGAACTTGCTGGCGGCGCTGGGATGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcc                                                                  <  1:1900550/93‑1 (MQ=255)
                       aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAAtcctc                                                                <  1:4493815/93‑1 (MQ=255)
                       aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctc                                                                >  2:2782117/1‑93 (MQ=255)
                        agaaCTTGCTGGGGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctcc                                                               <  1:3168882/93‑1 (MQ=255)
                        agaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctcc                                                               >  1:3895885/1‑93 (MQ=255)
                        agaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctcc                                                               <  1:4419464/93‑1 (MQ=255)
                         gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                              >  1:1225265/1‑93 (MQ=255)
                         gaaCTGGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                              <  2:2443831/93‑1 (MQ=255)
                          aaCTTGCTGGCGGCGCTGTGCTGCCAGTTTGATCTGCATAGTCTGGTAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAGCCTCcgc                                                             <  1:918559/93‑1 (MQ=255)
                          aaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgc                                                             <  1:918560/93‑1 (MQ=255)
                           aCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgcg                                                            <  2:3915975/93‑1 (MQ=255)
                           aCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcccg                                                            >  1:2617213/1‑93 (MQ=255)
                             ttGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                          >  2:1956504/1‑93 (MQ=255)
                             ttGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                          >  1:4046383/1‑93 (MQ=255)
                             ttGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                          >  2:1705234/1‑93 (MQ=255)
                             ttGCTGGCGCCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                          <  2:142535/93‑1 (MQ=255)
                              tGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGc                                                         <  2:1123295/93‑1 (MQ=255)
                              tGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGc                                                         <  1:3352878/93‑1 (MQ=255)
                               gCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCt                                                        <  1:1922086/93‑1 (MQ=255)
                               gCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCt                                                        <  1:2121141/93‑1 (MQ=255)
                                cTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTg                                                       >  1:2147704/1‑93 (MQ=255)
                                cTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTg                                                       >  1:2781610/1‑93 (MQ=255)
                                   gcggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATg                                                    <  2:2463814/93‑1 (MQ=255)
                                     ggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgcg                                                  >  1:4431604/1‑93 (MQ=255)
                                        gcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGa                                               >  2:2019095/1‑93 (MQ=255)
                                        gcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGGGGAAATCGTCCGCGGGCTGATGGGCGa                                               >  2:1160540/1‑93 (MQ=255)
                                           gggCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTc                                            >  2:762016/1‑93 (MQ=255)
                                            ggCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTcg                                           <  1:3491105/93‑1 (MQ=255)
                                             gCTGCCAGTTTGATCTGCATAGTCTGGCAGGACCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTcgc                                          <  2:1560799/93‑1 (MQ=255)
                                              cTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTcgcg                                         <  1:4461642/93‑1 (MQ=255)
                                               tGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCgg                                        >  2:403847/1‑93 (MQ=255)
                                               tGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCgg                                        >  2:3851814/1‑93 (MQ=255)
                                               tGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCgg                                        >  1:3078507/1‑93 (MQ=255)
                                               tGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCgg                                        >  2:1708384/1‑93 (MQ=255)
                                                gCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCCAATGGTGGAAATCCTCCGCGGGCTGATGTGTGACTCGCGGa                                       >  2:3326054/1‑93 (MQ=255)
                                                gCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGa                                       >  1:4159259/1‑93 (MQ=255)
                                                  cAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGAtt                                     >  1:730686/1‑93 (MQ=255)
                                                        gATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATc                               <  2:890750/93‑1 (MQ=255)
                                                         aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATct                              >  2:1453908/1‑92 (MQ=255)
                                                         aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                              >  1:1903854/1‑93 (MQ=255)
                                                          tCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGCTCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGGGCGACTCGCGGATTCTGATcac                             >  2:1153473/1‑91 (MQ=255)
                                                          tCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCt                             >  1:4747789/1‑93 (MQ=255)
                                                           cttcATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTc                            <  2:2205152/90‑1 (MQ=255)
                                                              cATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGAt                         <  1:1093582/93‑1 (MQ=255)
                                                              cATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGAt                         >  2:1675104/1‑93 (MQ=255)
                                                               aTAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATg                        <  2:214283/93‑1 (MQ=255)
                                                                tAGTCTGGCAGGATCGCTGGATGTCACCGACCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                       <  1:675632/93‑1 (MQ=255)
                                                                tAGTCTGGCAGGATCGCTGGATGTCACCGACCGCCAAATGGTGGAAAGCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                       <  1:675633/93‑1 (MQ=255)
                                                                 aGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGaa                      <  2:3783614/93‑1 (MQ=255)
                                                                  gTCTGGCAGGATAGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAc                     >  2:2946250/1‑93 (MQ=255)
                                                                   tCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAcc                    >  2:2126460/1‑93 (MQ=255)
                                                                    cTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCt                   <  2:1131027/93‑1 (MQ=255)
                                                                     tGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTa                  >  1:2257328/1‑93 (MQ=255)
                                                                      ggCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAc                 <  1:2083558/93‑1 (MQ=255)
                                                                       gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                <  2:4007248/93‑1 (MQ=255)
                                                                          ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc             <  2:4555351/93‑1 (MQ=255)
                                                                          ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc             <  2:1841106/93‑1 (MQ=255)
                                                                          ggATCCCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc             <  2:1770128/93‑1 (MQ=255)
                                                                           gATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCt            <  1:3294819/93‑1 (MQ=255)
                                                                           gATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCt            <  1:361559/93‑1 (MQ=255)
                                                                            aTCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTc           >  1:346531/1‑93 (MQ=255)
                                                                            aTCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTc           <  2:4105582/93‑1 (MQ=255)
                                                                             tCGCTGGATGTCACCGAGCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCg          <  2:3838437/93‑1 (MQ=255)
                                                                               gCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCt        >  2:2047425/1‑93 (MQ=255)
                                                                                 tGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGCACCTACCGCCTCGCTTa      >  2:2754395/1‑93 (MQ=255)
                                                                                 tGGATGTCACCGATCGCCAAATGGGGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAACGCCTCCCTTa      >  2:2754394/1‑93 (MQ=255)
                                                                                  ggATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAc     >  2:1867505/1‑93 (MQ=255)
                                                                                   gATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcc    >  2:2532500/1‑93 (MQ=255)
                                                                                     tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc  >  2:3190480/1‑93 (MQ=255)
                                                                                     tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc  >  1:4426306/1‑93 (MQ=255)
                                                                                     tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc  <  2:4329174/93‑1 (MQ=255)
                                                                                     tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc  >  2:3705352/1‑93 (MQ=255)
                                                                                         |                                                                                        
AACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCC  >  NC_000913/1599854‑1600031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑