Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,619,900 T→C V182A (GTA→GCA)  ydeF → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,619,9000TC99.5% 601.8 / ‑4.8 185V182A (GTA→GCA) ydeFputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (81/103);  total (82/103)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 193 total aligned reads displayed.

GCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGTAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGTTTA  >  NC_000913/1619810‑1619989
                                                                                          |                                                                                         
gCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCaa                                                                                         <  2:1343023/93‑1 (MQ=255)
 cACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCaaa                                                                                        <  1:135204/93‑1 (MQ=255)
 cACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCaaa                                                                                        <  2:1698852/93‑1 (MQ=255)
  aCGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAg                                                                                       >  1:101481/1‑93 (MQ=255)
  aCGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAg                                                                                       >  2:376866/1‑93 (MQ=255)
  aCGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAg                                                                                       >  2:2658450/1‑93 (MQ=255)
   cGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAgc                                                                                      <  2:3535509/93‑1 (MQ=255)
   cGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAgc                                                                                      <  1:2554529/93‑1 (MQ=255)
        ttGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGc                                                                                 <  2:2852776/93‑1 (MQ=255)
        gtGGTAATGCAGAGCATCAATCTGACCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCAAAGTTTTTCATTCAAATTTGGGCAAAGCGCAGc                                                                                 <  2:3646119/92‑1 (MQ=255)
         tGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCg                                                                                >  1:448649/1‑93 (MQ=255)
          ggTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCga                                                                               <  2:3514645/93‑1 (MQ=255)
          ggTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCga                                                                               >  1:494997/1‑93 (MQ=255)
            tAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCgaga                                                                             <  1:259279/93‑1 (MQ=255)
             aaTGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGaa                                                                            <  2:4606080/93‑1 (MQ=255)
              aTGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGaaa                                                                           >  1:2918657/1‑93 (MQ=255)
               tGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGaaaa                                                                          >  2:1527023/1‑93 (MQ=255)
               tGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGaaaa                                                                          <  1:4428128/93‑1 (MQ=255)
                gCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCAATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAat                                                                         <  2:4065285/93‑1 (MQ=255)
                  agagCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAatca                                                                       <  2:1757106/93‑1 (MQ=255)
                    agCATCAGTCTGCCCTTCTGGCTGGCAGCTGTCTGTTCCGTGTTTGCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAatcatc                                                                     <  1:4617854/93‑1 (MQ=255)
                     gCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCGGCGAGAAAATCATCg                                                                    >  2:1239783/1‑93 (MQ=255)
                      cATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGc                                                                   <  1:654734/93‑1 (MQ=255)
                      cATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGc                                                                   >  2:1994149/1‑93 (MQ=255)
                       aTCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGcc                                                                  <  2:2282176/93‑1 (MQ=255)
                       aTCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGcc                                                                  >  2:931770/1‑93 (MQ=255)
                        tCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCa                                                                 >  2:137343/1‑93 (MQ=255)
                        tCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCa                                                                 >  1:3488483/1‑93 (MQ=255)
                               gCCCTTATGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAAc                                                          >  1:3238398/1‑93 (MQ=255)
                                 ccTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAg                                                        <  1:1389288/93‑1 (MQ=255)
                                 ccTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAg                                                        <  1:602579/93‑1 (MQ=255)
                                  cTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAgg                                                       <  2:2835976/93‑1 (MQ=255)
                                  cTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAgg                                                       <  1:4659710/93‑1 (MQ=255)
                                   ttCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGACACGGAAACAGGc                                                      >  1:6452/1‑93 (MQ=255)
                                         ctggcAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTc                                                <  1:1082268/93‑1 (MQ=255)
                                          tggcAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCt                                               >  1:1267596/1‑93 (MQ=255)
                                          tggcAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCt                                               <  2:1958778/93‑1 (MQ=255)
                                          tggcAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCt                                               <  1:4444637/93‑1 (MQ=255)
                                            gcAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTgg                                             <  1:1062070/93‑1 (MQ=255)
                                              aGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTc                                           >  2:3529207/1‑93 (MQ=255)
                                               gCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCg                                          >  2:1669478/1‑93 (MQ=255)
                                               gCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCg                                          >  1:3252408/1‑93 (MQ=255)
                                               gCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCg                                          >  1:1210809/1‑93 (MQ=255)
                                                cTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGc                                         <  2:2941833/93‑1 (MQ=255)
                                                cTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGc                                         >  1:1989333/1‑93 (MQ=255)
                                                 tATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGcc                                        >  1:2815347/1‑93 (MQ=255)
                                                 tATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGcc                                        <  2:792686/93‑1 (MQ=255)
                                                   tCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGa                                      >  1:2894564/1‑93 (MQ=255)
                                                     tGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGaaa                                    <  1:3182245/93‑1 (MQ=255)
                                                      gTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAg                                   >  2:3691295/1‑93 (MQ=255)
                                                       ttCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGt                                  <  2:2403331/93‑1 (MQ=255)
                                                       ttCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGt                                  >  2:414335/1‑93 (MQ=255)
                                                        tCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGtt                                 >  1:4304145/1‑93 (MQ=255)
                                                         ccGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGttt                                >  2:3227607/1‑93 (MQ=255)
                                                         ccGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGttt                                <  2:3866469/93‑1 (MQ=255)
                                                         ccGCGTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGttt                                <  1:493918/93‑1 (MQ=255)
                                                           gcgTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTtta                              >  2:2228242/1‑93 (MQ=255)
                                                           gcgTGTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTtta                              <  2:1554205/93‑1 (MQ=255)
                                                            cgTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTttat                             <  2:4498292/93‑1 (MQ=255)
                                                             gTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTttatt                            >  1:2261887/1‑93 (MQ=255)
                                                             gTTTCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTttatt                            >  1:3033478/1‑93 (MQ=255)
                                                               ttCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTAc                          <  2:3184570/93‑1 (MQ=255)
                                                               ttCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTAc                          <  2:4514603/93‑1 (MQ=255)
                                                               ttCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTAc                          >  2:2520103/1‑93 (MQ=255)
                                                                tCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACa                         <  1:178876/93‑1 (MQ=255)
                                                                tCCCATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACa                         >  2:2161565/1‑93 (MQ=255)
                                                                 cccATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACaa                        >  2:3371641/1‑93 (MQ=255)
                                                                 cccATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACaa                        <  1:1006118/93‑1 (MQ=255)
                                                                  cgaTGCTTTTCACTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCCGGTCGCCGAAAGTTTTATTACAAg                       <  1:378671/91‑1 (MQ=255)
                                                                  ccATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAg                       >  2:483217/1‑93 (MQ=255)
                                                                  ccATGCTTTTCATTCAAATTTGGGCAAAGCGCAGAGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAg                       >  2:2977947/1‑93 (MQ=255)
                                                                   cATGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGa                      >  2:307346/1‑93 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     <  2:655210/93‑1 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     >  2:1032491/1‑93 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     >  2:1171291/1‑93 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     <  2:3417068/93‑1 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     <  1:2758143/93‑1 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     <  1:1703962/93‑1 (MQ=255)
                                                                    aTGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGAt                     <  1:1525544/93‑1 (MQ=255)
                                                                     tGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATa                    <  1:236729/93‑1 (MQ=255)
                                                                     tGCTTTTCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATa                    >  1:1262913/1‑93 (MQ=255)
                                                                        ttttCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAg                 <  2:1881193/93‑1 (MQ=255)
                                                                         tttCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGc                <  1:2745652/93‑1 (MQ=255)
                                                                         tttCATTCAAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTAGCCGAAAGTTTTATTAGAAGATAAAga                >  2:3058061/1‑92 (MQ=255)
                                                                            cattcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACt             <  1:3176218/93‑1 (MQ=255)
                                                                            cattcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACt             <  2:852969/93‑1 (MQ=255)
                                                                            cattcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACCGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACt             <  1:130360/93‑1 (MQ=255)
                                                                             attcaAACTTGGGAAAAGATCAGGGAGAAACTCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACtg            <  1:2462901/93‑1 (MQ=255)
                                                                              ttcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACtgt           >  2:1272862/1‑93 (MQ=255)
                                                                              ttcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACtgt           >  1:4559742/1‑93 (MQ=255)
                                                                               tcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACtgtt          <  1:946225/93‑1 (MQ=255)
                                                                               tcaAATTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACtgtt          <  2:4000325/93‑1 (MQ=255)
                                                                                 aaaTTTGGGCAAAGCGCAGCGAGAAAATCATCGCTACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCAAtgttgt        <  2:2457939/93‑1 (MQ=255)
                                                                                 aaaTTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCAAtgttgt        <  1:3147412/93‑1 (MQ=255)
                                                                                  aaTTTGGGCAAAGGGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTCCAAGATAAAGCACTGTtgtg       <  1:221211/93‑1 (MQ=255)
                                                                                  aaTTTGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTtgtg       <  1:221331/93‑1 (MQ=255)
                                                                                   aTTTGCGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTgg      <  2:3703677/93‑1 (MQ=255)
                                                                                    tttGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGt     >  1:4093044/1‑93 (MQ=255)
                                                                                    tttGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGt     <  1:782665/93‑1 (MQ=255)
                                                                                    tttGGGCAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGt     <  2:2325388/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGCGTTTCCCATGCTTTTCATTCAAATTTGGGTAAAGCGCAGCGAGAAAATCATCGCCACGGAAACAGGCAGTGTCTGGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGTTTA  >  NC_000913/1619810‑1619989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑