BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,632,141	T→C	S166P (TCA→CCA)	b1545 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,632,141	0	T	C	99.0%	287.6 / ‑4.8	101	S166P (TCA→CCA)	b1545	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (46/54); total (47/54)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 101 total aligned reads displayed.

ATAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGTCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGCA  >  NC_000913/1632051‑1632212

aTAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCa                                                                       <  1:2741802/93‑1 (MQ=255)

aTAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCa                                                                       <  2:1823073/93‑1 (MQ=255)

aTAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCa                                                                       <  2:241338/93‑1 (MQ=255)

aTAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCa                                                                       <  1:3771200/93‑1 (MQ=255)

 tAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAg                                                                      >  2:1181002/1‑93 (MQ=37)

 tAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAg                                                                      <  1:640446/93‑1 (MQ=255)

   gTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGgt                                                                    <  1:731155/93‑1 (MQ=255)

    tAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGgtg                                                                   <  1:3895638/93‑1 (MQ=255)

     aaGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGgtgt                                                                  <  2:3645251/93‑1 (MQ=255)

       gagCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTaa                                                                <  2:3153096/93‑1 (MQ=255)

       gagCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTaa                                                                >  1:2387022/1‑93 (MQ=37)

        agCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAg                                                               <  2:2486939/93‑1 (MQ=255)

        agCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAg                                                               <  1:4328341/93‑1 (MQ=255)

         gCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGt                                                              >  1:3748453/1‑93 (MQ=37)

         gCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGt                                                              >  1:448534/1‑93 (MQ=37)

          cccGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGta                                                             >  2:3568304/1‑93 (MQ=37)

          cccGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGta                                                             >  1:4629396/1‑93 (MQ=37)

          cccGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGta                                                             <  2:4161506/93‑1 (MQ=255)

           ccGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGtat                                                            >  1:3900504/1‑93 (MQ=255)

              cggcgCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAg                                                         <  2:1408379/93‑1 (MQ=255)

              cggcgCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAg                                                         <  2:1390423/93‑1 (MQ=255)

                 cgcAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGc                                                      <  2:3140136/93‑1 (MQ=255)

                  gcAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGcc                                                     >  2:2054791/1‑93 (MQ=255)

                   cAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCa                                                    <  2:1859575/93‑1 (MQ=255)

                    aGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCAt                                                   >  1:463160/1‑93 (MQ=255)

                     gggAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCAtt                                                  >  2:369187/1‑93 (MQ=255)

                      ggAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTg                                                 <  2:3125579/93‑1 (MQ=255)

                      ggAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTg                                                 >  1:1423740/1‑93 (MQ=255)

                       gAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGc                                                <  2:3855228/93‑1 (MQ=255)

                       gAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGc                                                <  2:2377328/93‑1 (MQ=255)

                       gAAACGTGTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGc                                                <  2:3855227/93‑1 (MQ=255)

                        aaaCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTAcc                                               >  1:605830/1‑93 (MQ=255)

                        aaaCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGCGTAAGTATAAGTGCCATTGcc                                               >  1:2609786/1‑93 (MQ=255)

                         aaCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGTCAGGTGTAAGTATAAGTGCCATTGccc                                              >  1:2340204/1‑93 (MQ=37)

                         aaCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGccc                                              >  1:70713/1‑93 (MQ=255)

                         aaCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGccc                                              <  2:4073725/93‑1 (MQ=255)

                          aCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCg                                             >  1:1591945/1‑93 (MQ=255)

                          aCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGAGTAAGTATAAGTGCCATTGCCCg                                             >  2:767795/1‑93 (MQ=255)

                          aCGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCAGTATTCGAACGAATTAAACCAGGTGTAAGTATAAGTGCCATTGCCCg                                             >  1:3111452/1‑93 (MQ=14)

                           cGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCgg                                            <  2:4288568/93‑1 (MQ=255)

                            gTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCggg                                           >  2:1033099/1‑93 (MQ=255)

                            gTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCggg                                           >  2:760924/1‑93 (MQ=255)

                                 ggTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAAtta                                      >  1:3783639/1‑92 (MQ=255)

                                  gTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCa                                     <  2:1721178/93‑1 (MQ=255)

                                   tCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaa                                    <  2:317701/93‑1 (MQ=255)

                                   tCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaa                                    >  2:412744/1‑93 (MQ=255)

                                    cGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaaa                                   >  1:194432/1‑93 (MQ=255)

                                     gACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaaaa                                  <  2:4107166/93‑1 (MQ=255)

                                     gACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaaaa                                  <  1:1464784/93‑1 (MQ=255)

                                     gACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaaaa                                  >  1:4744410/1‑93 (MQ=37)

                                     gACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCaaaa                                  >  2:3228755/1‑93 (MQ=255)

                                        caccTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCt                               <  1:2592111/93‑1 (MQ=25)

                                        caccTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCt                               <  1:2816546/93‑1 (MQ=25)

                                        caccTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCt                               >  2:2841584/1‑93 (MQ=25)

                                        caccTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCt                               <  1:1491480/93‑1 (MQ=25)

                                         accTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTc                              >  1:2115153/1‑93 (MQ=25)

                                         accTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTc                              <  2:1554431/93‑1 (MQ=21)

                                         accTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTc                              >  1:2983941/1‑93 (MQ=25)

                                           cTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgc                            <  1:4279554/93‑1 (MQ=18)

                                           cTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgc                            <  2:4464518/93‑1 (MQ=18)

                                           cTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgc                            <  1:2844722/93‑1 (MQ=18)

                                           cTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgc                            <  1:453284/93‑1 (MQ=18)

                                            tgtgTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgcg                           >  2:1048490/1‑93 (MQ=21)

                                            tgtgTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTcgcg                           >  1:1714537/1‑93 (MQ=21)

                                             gtgtTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCgg                          >  2:3994651/1‑93 (MQ=21)

                                              tgtTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGc                         >  1:2165465/1‑93 (MQ=21)

                                              tgtTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGc                         >  1:461989/1‑93 (MQ=18)

                                                 tAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCaac                      >  1:1724722/1‑92 (MQ=21)

                                                  aaaTGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAAc                     <  2:1765516/93‑1 (MQ=18)

                                                  aaaTGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGAAAAc                     >  1:4155502/1‑93 (MQ=16)

                                                    aTGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCa                   <  1:1631687/93‑1 (MQ=18)

                                                     tGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAt                  <  2:4562630/93‑1 (MQ=18)

                                                     tGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAt                  >  2:1044450/1‑93 (MQ=21)

                                                      gaagaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAtt                 >  1:724117/1‑93 (MQ=21)

                                                      gaagaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAtt                 <  1:4326722/93‑1 (MQ=18)

                                                       aagaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAttt                <  2:4050416/93‑1 (MQ=18)

                                                       aagaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAttt                <  2:4050415/93‑1 (MQ=18)

                                                        agaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCAtttt               <  2:3277920/93‑1 (MQ=18)

                                                         gaaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTa              >  1:4236657/1‑93 (MQ=21)

                                                          aaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTaa             <  1:247643/93‑1 (MQ=18)

                                                          aaCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTaa             <  2:4481446/93‑1 (MQ=18)

                                                           aCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAag            >  2:585733/1‑93 (MQ=18)

                                                           aCAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAag            >  1:4364929/1‑93 (MQ=18)

                                                            cAGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAaga           <  2:2310682/93‑1 (MQ=18)

                                                             aGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAagag          <  2:1683823/93‑1 (MQ=18)

                                                             aGAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAagag          <  2:582392/93‑1 (MQ=18)

                                                              gAAACAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGc         >  2:3667703/1‑93 (MQ=18)

                                                               aaaCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGcc        >  2:3942763/1‑93 (MQ=21)

                                                               aaaCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGcc        >  1:995932/1‑93 (MQ=18)

                                                               aaaCAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGcc        >  2:176590/1‑93 (MQ=21)

                                                                  cAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCaaa     <  1:1620218/93‑1 (MQ=18)

                                                                  cAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCaaa     <  2:258621/93‑1 (MQ=18)

                                                                  cAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCaaa     <  2:2451603/93‑1 (MQ=18)

                                                                  cAGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCAGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCaaa     <  2:475160/93‑1 (MQ=255)

                                                                   aGGCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAg    <  1:244360/93‑1 (MQ=18)

                                                                    ggCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGc   <  1:1196965/93‑1 (MQ=18)

                                                                    ggCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGc   <  2:2556692/93‑1 (MQ=18)

                                                                     gCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGCa  >  1:1242719/1‑93 (MQ=17)

                                                                     gCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGCa  >  1:499293/1‑93 (MQ=17)

                                                                     gCGGTATTCGAACGAATTAAGCCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGCa  >  2:1996428/1‑93 (MQ=17)

ATAGTAAGAGCCCGCGGCGCAGGGAAACGTTTTGGTCGACCACCTGTGTTAAATGAAGAACAGAAACAGGCGGTATTCGAACGAATTAAGTCAGGTGTAAGTATAAGTGCCATTGCCCGGGAATTCAAAACCTCGCGGCAAACCATTTTAAGAGCCAAAGCA  >  NC_000913/1632051‑1632212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`