BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,693,549	A→G	W183R (TGG→CGG)	uidA ←	beta‑D‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,549	0	A	G	99.2%	422.7 / ‑4.6	134	W183R (TGG→CGG)	uidA	beta‑D‑glucuronidase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (53/80); total (54/80)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 139 total aligned reads displayed.

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638

 gACCTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTTCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                        >  1:3309468/1‑93 (MQ=255)

 gACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                        >  1:399659/1‑93 (MQ=255)

 gACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                        >  1:3309467/1‑93 (MQ=255)

   cATCACCATTGGCCCCCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGTGTCACCACGGTGATATCGTCCACCCGGgtgt                                                                                      <  2:270021/93‑1 (MQ=255)

    aTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     >  1:247250/1‑93 (MQ=255)

     tCACCATTGGCCCCCCCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTc                                                                                    <  1:1954761/93‑1 (MQ=255)

      cACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCg                                                                                   >  1:4727032/1‑93 (MQ=255)

       aCCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCCCCCGGGGGTTCgg                                                                                  >  1:1602662/1‑93 (MQ=255)

        ccATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGc                                                                                 >  1:1123981/1‑93 (MQ=255)

        ccATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGc                                                                                 <  2:4614374/93‑1 (MQ=255)

         cATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCg                                                                                >  2:4646563/1‑93 (MQ=255)

         cATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCg                                                                                >  1:1193053/1‑93 (MQ=255)

          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                               <  1:2139455/93‑1 (MQ=255)

          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgg                                                                               >  1:3798447/1‑92 (MQ=255)

           ttGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtg                                                                              <  1:4397357/93‑1 (MQ=255)

             ggCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggt                                                                            <  2:1938648/93‑1 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         <  1:1648618/93‑1 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         >  1:4744317/1‑93 (MQ=255)

                 accaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                        >  1:85274/1‑93 (MQ=255)

                 accaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGCGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                        >  1:1900736/1‑93 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  2:1770023/93‑1 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  1:86399/93‑1 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  1:924543/93‑1 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGCCGTGGTGTaga                                                                       <  2:3479389/93‑1 (MQ=255)

                    cccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGc                                                                     <  2:4118005/92‑1 (MQ=255)

                     ccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCa                                                                    <  2:3185348/93‑1 (MQ=255)

                     ccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCa                                                                    <  1:2747211/93‑1 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                  <  1:202524/93‑1 (MQ=255)

                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                 <  2:3786003/93‑1 (MQ=255)

                         ccAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAc                                                                <  2:3081989/93‑1 (MQ=255)

                         ccAGTCAACAGACGCGTGGTTACAGCCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAc                                                                <  2:4704088/93‑1 (MQ=255)

                          cAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACg                                                               <  1:611728/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  2:3492253/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  2:1211607/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  2:2637043/93‑1 (MQ=255)

                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                             <  1:1009762/93‑1 (MQ=255)

                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                            >  2:2131214/1‑93 (MQ=255)

                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                            >  1:4586972/1‑93 (MQ=255)

                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  2:4509187/93‑1 (MQ=255)

                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  1:4187666/93‑1 (MQ=255)

                               aaCAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCg                                                          >  2:2041735/1‑93 (MQ=255)

                                aCAGACGCGTGGTTACAGTCTTGCTCGACATTCGTCACCACCGTGTTTTCGTCCACCCGGGTGGTTGGCGCGTTGTAGTCCATTTCGCTGcat                                                         >  2:3168929/1‑91 (MQ=255)

                                aCAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTGCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                         <  1:2436361/93‑1 (MQ=255)

                                 cAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGAt                                                        >  1:4415327/1‑93 (MQ=255)

                                  aGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATg                                                       >  1:441552/1‑93 (MQ=255)

                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                      >  1:1946683/1‑93 (MQ=255)

                                      gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATc                                                   <  1:2965113/93‑1 (MQ=255)

                                      gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATc                                                   <  1:1404269/93‑1 (MQ=255)

                                      gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATc                                                   >  2:2412436/1‑93 (MQ=255)

                                       cgTGGTTACAGTCTTGAGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATcc                                                  <  2:470649/93‑1 (MQ=255)

                                          ggTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCgg                                               >  1:2610688/1‑93 (MQ=255)

                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCgga                                              >  2:2133327/1‑92 (MQ=255)

                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGc                                              >  1:4582986/1‑93 (MQ=255)

                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGc                                              >  1:881668/1‑93 (MQ=255)

                                            ttACAGTCTTGCGCAACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCa                                             >  1:4215371/1‑93 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                           <  1:4245841/93‑1 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                           <  1:4307602/93‑1 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                           <  1:2452329/93‑1 (MQ=255)

                                               cAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAg                                          >  1:131820/1‑93 (MQ=255)

                                               cAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAg                                          >  1:1760105/1‑93 (MQ=255)

                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGTTGGATCCCGGCATAGtt                                        >  2:3948482/1‑93 (MQ=255)

                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGtt                                        >  2:1558079/1‑93 (MQ=255)

                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGa                                   <  1:3529297/93‑1 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                >  2:3955376/1‑93 (MQ=255)

                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               <  1:2067145/93‑1 (MQ=255)

                                                           aCATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              <  2:592978/93‑1 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                             >  2:1019534/1‑93 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                             <  1:2434904/93‑1 (MQ=255)

                                                             aTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            <  1:2185712/93‑1 (MQ=255)

                                                               gCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGa                          <  2:4185854/93‑1 (MQ=255)

                                                               gCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGa                          <  2:1371571/93‑1 (MQ=255)

                                                                cGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGaa                         <  2:475424/93‑1 (MQ=255)

                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                        <  1:275115/93‑1 (MQ=255)

                                                                   caccacGGTGATATCGTCCACCCGGCTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTa                      <  2:403269/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     <  2:2499730/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     <  2:2308966/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     >  1:1753097/1‑93 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     >  1:1575945/1‑93 (MQ=255)

                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  >  2:4323872/1‑93 (MQ=255)

                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  >  1:3665756/1‑93 (MQ=255)

                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc               <  2:3413097/93‑1 (MQ=255)

                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc               <  2:3413098/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  2:436852/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  2:730456/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              >  2:1797386/1‑93 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  1:3231406/93‑1 (MQ=255)

                                                                           gtatatCGTCCAACCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  1:1089359/91‑1 (MQ=255)

                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt             <  2:4745925/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           <  2:4077679/93‑1 (MQ=255)

                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTc         >  2:346276/1‑93 (MQ=255)

                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTc         <  2:2023365/93‑1 (MQ=255)

                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTc         <  1:2194383/93‑1 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  1:2870221/93‑1 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  1:1508582/93‑1 (MQ=255)

                                                                                  gTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt       <  1:124387/93‑1 (MQ=255)

                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc    >  1:4273832/1‑93 (MQ=255)

                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc    <  1:25569/93‑1 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGt  >  2:676496/1‑93 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTATTTCGTGCCGt  <  2:4175503/93‑1 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATAATGGAAGTAAGACTGCTTTTTCTTGCCGt  <  1:401396/93‑1 (MQ=255)

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`