BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,480	A→G	F256S (TTC→TCC)	tyrS ←	tyrosine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,480	0	A	G	98.6%	440.2 / ‑5.4	138	F256S (TTC→TCC)	tyrS	tyrosine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (72/64); total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGC  >  NC_000913/1714390‑1714569

aGGGGGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCTGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                         <  1:3775627/93‑1 (MQ=255)

aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                         >  2:4109754/1‑93 (MQ=255)

aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                         >  1:656225/1‑93 (MQ=255)

aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                         <  1:3775626/93‑1 (MQ=255)

 gggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAtt                                                                                        <  1:1057425/93‑1 (MQ=255)

 gggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAtt                                                                                        >  2:2806124/1‑93 (MQ=255)

 gggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAtt                                                                                        <  1:898359/93‑1 (MQ=255)

  ggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAttt                                                                                       <  1:4517425/93‑1 (MQ=255)

     gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTa                                                                                    >  2:764907/1‑93 (MQ=255)

     gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTa                                                                                    >  2:3208103/1‑93 (MQ=255)

     gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTa                                                                                    >  1:2369228/1‑93 (MQ=255)

           ttctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                              <  1:124331/92‑1 (MQ=255)

           ctctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                              <  1:1617135/93‑1 (MQ=255)

           ctctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                              >  1:4065443/1‑93 (MQ=255)

           atctGCAATGCTAATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                              <  2:1820598/92‑1 (MQ=255)

             ctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTgg                                                                            <  2:1124383/93‑1 (MQ=255)

             ctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTgg                                                                            <  1:2231997/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                           <  2:3650956/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                           >  2:2029232/1‑93 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                           >  2:1168416/1‑93 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                           >  2:1168415/1‑93 (MQ=255)

                cAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGttt                                                                         >  1:3438700/1‑93 (MQ=255)

                cAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGttt                                                                         >  1:693676/1‑93 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                        >  2:644458/1‑93 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                        >  2:587039/1‑93 (MQ=255)

                  aTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTttc                                                                       <  2:970195/93‑1 (MQ=255)

                  aTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTttc                                                                       <  2:1737276/93‑1 (MQ=255)

                  aTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTttc                                                                       >  1:3733725/1‑93 (MQ=255)

                   tGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTttct                                                                      >  1:4633334/1‑93 (MQ=255)

                     cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTttcttc                                                                    <  1:2401168/93‑1 (MQ=255)

                      tCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCg                                                                   >  2:108124/1‑93 (MQ=255)

                       cATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCgg                                                                  <  1:3934222/93‑1 (MQ=255)

                       cATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCgg                                                                  <  1:3934223/93‑1 (MQ=255)

                        aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGa                                                                 >  2:686137/1‑93 (MQ=255)

                         tAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAt                                                                >  1:2798075/1‑93 (MQ=255)

                          aaaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATc                                                               <  1:3802432/93‑1 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                              <  1:579549/93‑1 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                              <  1:361210/93‑1 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                              >  2:2030085/1‑93 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                              >  1:2164178/1‑93 (MQ=255)

                                gaagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCa                                                         >  2:1233816/1‑93 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                        <  1:3382115/93‑1 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                        >  1:1939151/1‑93 (MQ=255)

                                    aaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                                     >  1:1875694/1‑93 (MQ=255)

                                       ttCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcg                                                  >  2:879511/1‑93 (MQ=255)

                                       ttCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATACAACCAGACTgcg                                                  >  1:2108046/1‑93 (MQ=255)

                                           ggAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccgc                                              >  1:673254/1‑93 (MQ=255)

                                           ggAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccgc                                              >  2:299404/1‑93 (MQ=255)

                                           ggAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccgc                                              >  1:3566796/1‑93 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                            <  2:3597910/93‑1 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                            <  1:2806561/93‑1 (MQ=255)

                                              aGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                                           <  2:4084389/93‑1 (MQ=255)

                                               gCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTc                                          <  1:3016348/93‑1 (MQ=255)

                                                cGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCa                                         <  1:3922206/93‑1 (MQ=255)

                                                 ggTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCCCCGCCTTCAg                                        <  2:3303753/93‑1 (MQ=255)

                                                   tAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtt                                      <  2:2505651/93‑1 (MQ=255)

                                                    aaaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGATTGCGCCGCCTTCAGttt                                     <  2:2619458/93‑1 (MQ=255)

                                                    aaaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                                     <  2:2527472/93‑1 (MQ=255)

                                                        gTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAcc                                 >  2:2919434/1‑93 (MQ=255)

                                                        gTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAcc                                 <  2:2380337/93‑1 (MQ=255)

                                                        gTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAca                                 >  1:4416578/1‑92 (MQ=255)

                                                         tCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCa                                >  2:4244864/1‑93 (MQ=255)

                                                         tCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCa                                >  2:1079771/1‑93 (MQ=255)

                                                          cGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCaa                               <  2:701739/93‑1 (MQ=255)

                                                           ggCATGCGCAGTGTTGATCCAGAACTGGTAAAATATGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGACGCCTTCAGTATTACCaaa                              <  2:4353498/93‑1 (MQ=255)

                                                           ggCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCaaa                              <  1:2581002/93‑1 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAt                             >  1:782503/1‑93 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAt                             <  2:3368121/93‑1 (MQ=255)

                                                              aTCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt                           <  2:2893683/93‑1 (MQ=255)

                                                              aTCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt                           >  2:588503/1‑93 (MQ=255)

                                                                ccGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTgg                         <  2:965885/93‑1 (MQ=255)

                                                                ccGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTgg                         <  1:1042762/93‑1 (MQ=255)

                                                                ccGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTgg                         <  1:136365/93‑1 (MQ=255)

                                                                  gCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg                       >  1:4288540/1‑93 (MQ=255)

                                                                  gCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg                       >  1:2197226/1‑93 (MQ=255)

                                                                    aGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGGTCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                     >  2:3409922/1‑93 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                    <  2:3364383/93‑1 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                    >  2:48297/1‑93 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                    >  2:48296/1‑93 (MQ=255)

                                                                      tgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCAt                   >  2:2084343/1‑93 (MQ=255)

                                                                        ttGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                 <  1:1544593/93‑1 (MQ=255)

                                                                            tCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt             >  2:1761632/1‑93 (MQ=255)

                                                                            tCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt             <  1:2618609/93‑1 (MQ=255)

                                                                            tCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt             >  1:835774/1‑93 (MQ=255)

                                                                              cAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTa           <  2:4471833/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGACATCTGCTTTAg          >  2:895311/1‑93 (MQ=255)

                                                                                gAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGt         >  1:4549102/1‑93 (MQ=255)

                                                                                 aaCTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTg        <  2:752151/93‑1 (MQ=255)

                                                                                  aCTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGa       <  2:1310457/93‑1 (MQ=255)

                                                                                  aCTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGa       >  1:3732140/1‑93 (MQ=255)

                                                                                   cTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGAt      >  2:3798604/1‑93 (MQ=255)

                                                                                   cTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGAt      >  2:3905225/1‑93 (MQ=255)

                                                                                    tGGTAGGATTTGTACGGTCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATc     <  1:3746036/93‑1 (MQ=255)

                                                                                    tGGTAGGATTTGTACGGGCTTGTTTTGTTTGGATCCAACCAGACTGAGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATc     <  1:4080526/93‑1 (MQ=255)

                                                                                    tGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATc     >  1:1753071/1‑93 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa    <  2:3380229/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa    <  2:2428513/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa    >  1:3836803/1‑93 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg   >  2:3513567/1‑93 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTCGGTGCCATCTGCTTTAGTGATCAg   <  2:4449442/93‑1 (MQ=255)

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGC  >  NC_000913/1714390‑1714569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`