BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,150	A→G	T88T (ACT→ACC)	ydhA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,150	0	A	G	98.8%	504.9 / ‑2.6	161	T88T (ACT→ACC)	ydhA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (93/66); total (94/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

Only 100 of 166 total aligned reads displayed.

CGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716061‑1716239

cGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTc                                                                                        >  1:4509080/1‑93 (MQ=255)

cGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTc                                                                                        <  2:2170587/93‑1 (MQ=255)

 gCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCg                                                                                       >  2:1911426/1‑93 (MQ=255)

  cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      >  1:157065/1‑93 (MQ=255)

  cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      >  2:1757198/1‑93 (MQ=255)

   tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                     >  2:1844234/1‑93 (MQ=255)

    aTTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCtt                                                                                    <  1:1223381/93‑1 (MQ=255)

     ttGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTc                                                                                   <  2:133601/93‑1 (MQ=255)

        tgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                                <  2:2449436/93‑1 (MQ=255)

        tgCGCCCACCCAGCAAAAAGCCCAACGCTGTGGATTTTGTAACTAACAGTTATTCAAGACGATGCGGTCGTTTTTATAGACGGTCGCTTCATc                                                                                <  2:2106831/93‑1 (MQ=255)

          cgccgcCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGc                                                                              <  1:709132/93‑1 (MQ=255)

             cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           <  2:206858/93‑1 (MQ=255)

                cccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCg                                                                        >  1:3927983/1‑93 (MQ=255)

                 ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       >  1:111761/1‑93 (MQ=255)

                 ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       >  2:1566713/1‑93 (MQ=255)

                 ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       >  2:1970020/1‑93 (MQ=255)

                 ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       >  1:189352/1‑93 (MQ=255)

                 ccTGGAAAAATCTCAACGCTGTGGAGTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCgc                                                                       >  1:1557663/1‑92 (MQ=255)

                  cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  1:2275083/1‑93 (MQ=255)

                  cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  1:2945481/1‑93 (MQ=255)

                  cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  1:3038972/1‑93 (MQ=255)

                  cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  2:3169006/1‑93 (MQ=255)

                   tGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAcc                                                                     >  2:2368098/1‑93 (MQ=255)

                   tGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAcc                                                                     >  2:4208653/1‑93 (MQ=255)

                    ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                    >  2:1270480/1‑93 (MQ=255)

                    ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                    <  2:1156178/93‑1 (MQ=255)

                    ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                    <  2:731664/93‑1 (MQ=255)

                    ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                    >  1:3576701/1‑93 (MQ=255)

                    ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                    >  1:3457167/1‑93 (MQ=255)

                     gAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAg                                                                   >  2:3495430/1‑93 (MQ=255)

                       aaaaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGaa                                                                 >  2:2505967/1‑93 (MQ=255)

                        aaaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCTCTTCATCGCCTTTCGACCAGaaa                                                                >  2:2604679/1‑93 (MQ=255)

                         aaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGaaaa                                                               >  2:2332740/1‑93 (MQ=255)

                          aTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGTTCGCTTCAGCGCCTGTCGACCAGaaacc                                                              >  1:1461589/1‑91 (MQ=255)

                          aTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAAc                                                              >  1:4719698/1‑93 (MQ=255)

                               aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         >  2:2878544/1‑93 (MQ=255)

                               aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         <  1:723742/93‑1 (MQ=255)

                               aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         <  2:3765024/93‑1 (MQ=255)

                               aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         >  1:3897044/1‑93 (MQ=255)

                                aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAt                                                        >  2:4337092/1‑93 (MQ=255)

                                aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGACTTTCGACCAGAAAACATAGAt                                                        >  1:1224050/1‑93 (MQ=255)

                                 cGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAtt                                                       <  1:1502351/93‑1 (MQ=255)

                                   cTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTcc                                                     >  1:2849081/1‑93 (MQ=255)

                                   cTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTcc                                                     <  1:2776270/93‑1 (MQ=255)

                                     gtgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGt                                                   >  2:3004100/1‑93 (MQ=255)

                                      tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  <  1:531801/93‑1 (MQ=255)

                                       ggATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCa                                                 <  1:4585648/93‑1 (MQ=255)

                                         aTTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgt                                               <  2:1881882/93‑1 (MQ=255)

                                           tttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtga                                             >  2:3826790/1‑92 (MQ=255)

                                            tggTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTa                                            <  2:2285254/91‑1 (MQ=255)

                                              gTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAc                                          >  2:3902819/1‑93 (MQ=255)

                                              gTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAc                                          >  2:3422084/1‑93 (MQ=255)

                                              gTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAc                                          <  1:3181263/93‑1 (MQ=255)

                                              gTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAc                                          <  2:161489/93‑1 (MQ=255)

                                                 aCTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcg                                       >  2:1270805/1‑93 (MQ=255)

                                                   tGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcgcg                                     >  2:3044967/1‑93 (MQ=255)

                                                     aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   <  2:2736848/93‑1 (MQ=255)

                                                       aGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCag                                 >  2:3795825/1‑93 (MQ=255)

                                                       aGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCag                                 >  2:4535172/1‑93 (MQ=255)

                                                       aGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCag                                 >  1:879689/1‑93 (MQ=255)

                                                        gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaga                                <  2:2693359/93‑1 (MQ=255)

                                                           tttaaaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                             <  1:2024718/89‑1 (MQ=255)

                                                           aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAggt                             >  1:4081600/1‑92 (MQ=255)

                                                            ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                            >  2:1636880/1‑93 (MQ=255)

                                                            ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                            >  1:881430/1‑93 (MQ=255)

                                                            ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                            >  1:2388329/1‑93 (MQ=255)

                                                             tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:4231033/1‑93 (MQ=255)

                                                             tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:4231034/1‑93 (MQ=255)

                                                               aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTTATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         <  2:499393/93‑1 (MQ=255)

                                                               aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         >  2:3551148/1‑93 (MQ=255)

                                                                aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        <  2:2506983/93‑1 (MQ=255)

                                                                 gACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAAt                       <  2:2075385/93‑1 (MQ=255)

                                                                   cGATGCGGGCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGGGTAACGCGCGCCAGAGGCTGAAATGc                     >  2:3293980/1‑93 (MQ=255)

                                                                    gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                    >  2:4578874/1‑93 (MQ=255)

                                                                     aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   <  2:3632776/93‑1 (MQ=255)

                                                                     aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   <  2:365412/93‑1 (MQ=255)

                                                                     aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   <  2:2421352/93‑1 (MQ=255)

                                                                      tGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCt                  >  1:2551358/1‑93 (MQ=255)

                                                                      tGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCt                  >  1:404221/1‑93 (MQ=255)

                                                                       gCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTg                 <  2:4018474/93‑1 (MQ=255)

                                                                       gCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTg                 >  1:763007/1‑93 (MQ=255)

                                                                        cGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGt                <  1:3777350/93‑1 (MQ=255)

                                                                         ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt               >  1:2537287/1‑93 (MQ=255)

                                                                          gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        >  1:3792699/1‑83 (MQ=255)

                                                                          gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              <  2:32393/93‑1 (MQ=255)

                                                                          gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              <  1:3099187/93‑1 (MQ=255)

                                                                          gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              >  2:642196/1‑93 (MQ=255)

                                                                          gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              <  1:4470358/93‑1 (MQ=255)

                                                                          gTCGCGTTGATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTACCGCGCGCCAGAGGCTGAAATGCCCTGttt              <  2:3976831/93‑1 (MQ=255)

                                                                            cgcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTga            <  1:1145077/93‑1 (MQ=255)

                                                                              cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          <  2:445025/93‑1 (MQ=255)

                                                                               gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt         <  1:1508424/93‑1 (MQ=255)

                                                                                 ttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGc       >  1:2883973/1‑93 (MQ=255)

                                                                                 ttATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGc       <  1:2009011/93‑1 (MQ=255)

                                                                                  tataGACGGTCGCTTCATCGCCTGTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCa      <  1:2376244/93‑1 (MQ=255)

                                                                                   ataGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCag     <  2:3876127/93‑1 (MQ=255)

                                                                                    taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    >  1:2416384/1‑93 (MQ=255)

                                                                                    taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    >  1:672448/1‑93 (MQ=255)

                                                                                     aGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagta   <  1:3422580/93‑1 (MQ=255)

                                                                                      gACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagtag  <  1:1665442/93‑1 (MQ=255)

CGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716061‑1716239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`