Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,738,433 T→C F167L (TTT→CTT)  ydhC → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,738,4330TC98.4% 382.9 / ‑2.6 121F167L (TTT→CTT) ydhCputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (70/49);  total (71/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 123 total aligned reads displayed.

CCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT  >  NC_000913/1738343‑1738522
                                                                                          |                                                                                         
ccGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCCCCCTGctt                                                                                         <  2:250957/93‑1 (MQ=255)
      gtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAtt                                                                                   <  2:2827101/93‑1 (MQ=255)
       tggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTa                                                                                  <  1:4744923/93‑1 (MQ=255)
          gTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCg                                                                               <  1:4260162/93‑1 (MQ=255)
            cTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtg                                                                             >  1:2778692/1‑93 (MQ=255)
             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            <  2:1194350/93‑1 (MQ=255)
             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            >  2:3275405/1‑93 (MQ=255)
             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            >  2:3275404/1‑93 (MQ=255)
              aTCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggt                                                                           >  2:4018111/1‑93 (MQ=255)
              aTCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggt                                                                           <  1:4540621/93‑1 (MQ=255)
               tctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggtg                                                                          >  2:4326207/1‑93 (MQ=255)
                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  1:694703/93‑1 (MQ=255)
                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         >  2:3164394/1‑93 (MQ=255)
                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  2:1064099/93‑1 (MQ=255)
                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  2:3080866/93‑1 (MQ=255)
                 tcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCt                                                                        >  2:4016544/1‑93 (MQ=255)
                 tcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCt                                                                        >  2:676572/1‑93 (MQ=255)
                  ccGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTg                                                                       >  1:382669/1‑93 (MQ=255)
                    ggCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAt                                                                     >  1:4052340/1‑93 (MQ=255)
                     gCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAtt                                                                    >  1:294316/1‑93 (MQ=255)
                      cACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTc                                                                   <  2:2934221/93‑1 (MQ=255)
                       aCTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCt                                                                  >  1:791963/1‑93 (MQ=255)
                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:4043995/93‑1 (MQ=255)
                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               >  2:4380947/1‑93 (MQ=255)
                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:493069/93‑1 (MQ=255)
                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:3605603/93‑1 (MQ=255)
                               ctctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTAttt                                                          >  2:1188133/1‑93 (MQ=255)
                                 ctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGTCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTAttttt                                                        >  1:1578922/1‑92 (MQ=255)
                                 ctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTc                                                        >  1:1578923/1‑93 (MQ=255)
                                  tGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCAATTTTCt                                                       <  1:741069/93‑1 (MQ=255)
                                     tAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGc                                                    >  2:1347264/1‑93 (MQ=255)
                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 <  2:1029211/93‑1 (MQ=255)
                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 >  1:67198/1‑93 (MQ=255)
                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 >  1:2636588/1‑93 (MQ=255)
                                         aaGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaa                                                >  1:3531747/1‑93 (MQ=255)
                                           gctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAc                                              <  2:620113/93‑1 (MQ=255)
                                           gctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAc                                              >  2:2773527/1‑93 (MQ=255)
                                              ggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCa                                           >  1:941829/1‑93 (MQ=255)
                                              ggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCa                                           <  2:2558726/93‑1 (MQ=255)
                                               gctgctGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac                                          <  2:1909958/93‑1 (MQ=255)
                                                ctgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacg                                         <  2:473321/93‑1 (MQ=255)
                                                ctgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacg                                         <  2:473320/93‑1 (MQ=255)
                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        <  1:1031781/93‑1 (MQ=255)
                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        <  1:1667219/93‑1 (MQ=255)
                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        >  2:1124356/1‑93 (MQ=255)
                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        >  1:2709083/1‑93 (MQ=255)
                                                  gctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgac                                       >  1:4236815/1‑93 (MQ=255)
                                                   ctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg                                      <  2:3181341/93‑1 (MQ=255)
                                                    tgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCAcgacga                                     >  1:4542774/1‑93 (MQ=255)
                                                     ggTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGaa                                    >  1:2098577/1‑93 (MQ=255)
                                                     ggTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCAACGACGaa                                    >  1:3617905/1‑93 (MQ=255)
                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAg                                   >  1:2768875/1‑93 (MQ=255)
                                                       tCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAgg                                  <  1:965434/93‑1 (MQ=255)
                                                       gccATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAgg                                  <  1:701895/92‑1 (MQ=255)
                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               >  1:2632937/1‑93 (MQ=255)
                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               <  1:3566140/93‑1 (MQ=255)
                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               >  1:207843/1‑93 (MQ=255)
                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGTCTCAAACCCACGACGAAGGCCCg                              >  2:2606657/1‑93 (MQ=255)
                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  2:1993434/93‑1 (MQ=255)
                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  1:4489755/93‑1 (MQ=255)
                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  2:1620795/93‑1 (MQ=255)
                                                            ttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGt                             >  2:3244449/1‑93 (MQ=255)
                                                              ttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTaa                           <  1:1024971/93‑1 (MQ=255)
                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  2:557403/1‑93 (MQ=255)
                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  1:1951326/1‑93 (MQ=255)
                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  2:4334215/1‑93 (MQ=255)
                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGACCGCAAc                          >  1:1951325/1‑93 (MQ=255)
                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCGATTACCGTGTTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                      >  2:4722211/1‑93 (MQ=255)
                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                      <  2:4673421/93‑1 (MQ=255)
                                                                     cAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAGCATAGt                    >  1:1994956/1‑93 (MQ=255)
                                                                      aGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTc                   <  2:3216745/93‑1 (MQ=255)
                                                                      aGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTc                   >  2:1348553/1‑93 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  2:3590960/1‑93 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  2:1891286/93‑1 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  2:4258791/93‑1 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  1:66179/1‑93 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  1:878244/1‑93 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  1:1546834/93‑1 (MQ=255)
                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  2:1978535/1‑93 (MQ=255)
                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               <  2:4136515/93‑1 (MQ=255)
                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               >  2:2581045/1‑93 (MQ=255)
                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               >  1:2058741/1‑93 (MQ=255)
                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  1:3776601/1‑93 (MQ=255)
                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  1:3975157/1‑93 (MQ=255)
                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  2:3075631/1‑93 (MQ=255)
                                                                            ttttCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATg             <  1:1059618/93‑1 (MQ=255)
                                                                                cGCCACCCTTCTGGCCATGACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCt         <  2:2433549/93‑1 (MQ=255)
                                                                                cGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCt         <  2:1775957/93‑1 (MQ=255)
                                                                                 gCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTg        >  1:4089898/1‑93 (MQ=255)
                                                                                 gCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTg        >  2:1600891/1‑93 (MQ=255)
                                                                                  ccACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGa       <  2:840044/93‑1 (MQ=255)
                                                                                   cccccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAc      <  1:1016920/91‑1 (MQ=255)
                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc     >  1:4595328/1‑93 (MQ=255)
                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc     >  1:4200311/1‑93 (MQ=255)
                                                                                     cccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCt    >  2:1116303/1‑93 (MQ=255)
                                                                                     cccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCt    >  2:4270440/1‑93 (MQ=255)
                                                                                      ccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCtt   >  2:310004/1‑93 (MQ=255)
                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  >  1:4609611/1‑93 (MQ=255)
                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  <  1:1034208/93‑1 (MQ=255)
                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  >  1:2915304/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT  >  NC_000913/1738343‑1738522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑