BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,758,261	T→C	E96G (GAA→GGA)	b1680 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,758,261	0	T	C	94.2%	290.9 / 6.3	103	E96G (GAA→GGA)	b1680	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (44/53); total (46/57)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTC  >  NC_000913/1758171‑1758349

ttAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGAtccctccc                                                                                        >  2:3088432/1‑93 (MQ=255)

ttAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGAtccctccc                                                                                        <  1:3671342/93‑1 (MQ=255)

 tAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcg                                                                                       <  2:736217/93‑1 (MQ=255)

  aGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgt                                                                                      >  2:3316477/1‑93 (MQ=255)

        gtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGcc                                                                                <  2:284737/93‑1 (MQ=255)

        gtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGcc                                                                                <  2:3379465/93‑1 (MQ=255)

         tgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcg                                                                               >  1:283981/1‑93 (MQ=255)

          gCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcgc                                                                              >  1:3926997/1‑93 (MQ=255)

             ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                           <  2:3435337/93‑1 (MQ=255)

              cATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGAc                                                                          <  2:3688965/93‑1 (MQ=255)

               aTCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACg                                                                         >  1:1088432/1‑93 (MQ=255)

               aTCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACg                                                                         >  1:444795/1‑93 (MQ=255)

               aTCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACg                                                                         <  1:223322/93‑1 (MQ=255)

                tCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGa                                                                        >  1:1275904/1‑93 (MQ=255)

                 ctgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGaa                                                                       <  2:3591656/93‑1 (MQ=255)

                   gactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAaca                                                                     <  2:1491167/93‑1 (MQ=255)

                   gactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAaca                                                                     >  1:768904/1‑93 (MQ=255)

                      tgatgatgaTGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCa                                                                  >  1:207718/1‑93 (MQ=255)

                       gatgatgatGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAg                                                                 <  2:4493425/93‑1 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGc                                                                <  2:299912/93‑1 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGc                                                                >  2:704269/1‑93 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGc                                                                <  2:3328232/93‑1 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGc                                                                <  1:185874/93‑1 (MQ=255)

                          gatgatgTTATCTCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                              <  2:2100478/93‑1 (MQ=255)

                          gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                              >  2:154684/1‑93 (MQ=255)

                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCtt                                                            >  2:3920506/1‑93 (MQ=255)

                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCtt                                                            <  2:750241/93‑1 (MQ=255)

                             gatgTTATCGCCCCCGCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                           <  1:3184241/93‑1 (MQ=255)

                                  tATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccgccg                                                      <  2:3800352/93‑1 (MQ=255)

                                  tATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccgccg                                                      <  1:2242237/93‑1 (MQ=255)

                                   aTCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGa                                                     <  2:3262124/93‑1 (MQ=255)

                                   aTCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGa                                                     >  2:1106388/1‑93 (MQ=255)

                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                    >  1:1868450/1‑93 (MQ=255)

                                     cgcccgccCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAAc                                                   <  2:1480204/93‑1 (MQ=255)

                                      gcccgcccGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACg                                                  <  2:3712299/93‑1 (MQ=255)

                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                 >  2:2016496/1‑93 (MQ=255)

                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                 <  2:4153450/93‑1 (MQ=255)

                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                 <  1:597171/93‑1 (MQ=255)

                                        ccgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACggg                                                <  2:1755232/93‑1 (MQ=255)

                                         cgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGc                                               >  1:3495489/1‑93 (MQ=255)

                                         cgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGc                                               <  1:4634868/93‑1 (MQ=255)

                                          gcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa                                              <  1:971727/93‑1 (MQ=255)

                                            ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGACCCCCAGCTCTTCCGCCGAACGGGCAtt                                            >  1:45872/1‑93 (MQ=255)

                                            ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAtt                                            >  2:730850/1‑93 (MQ=255)

                                            ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAtt                                            >  1:45873/1‑93 (MQ=255)

                                            ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAtt                                            <  1:2608517/93‑1 (MQ=255)

                                             cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCGGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta                                           <  2:4212043/93‑1 (MQ=255)

                                             cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta                                           >  1:3761053/1‑93 (MQ=255)

                                             cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta                                           >  1:1970732/1‑93 (MQ=255)

                                              gcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaa                                          >  1:4658195/1‑93 (MQ=255)

                                               cACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaat                                         >  2:869895/1‑93 (MQ=255)

                                                aCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                        >  1:3812340/1‑93 (MQ=255)

                                                 cGTTGCTGTTGCCCCAGCTATTGGCGACCCGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaataa                                       <  1:1038054/93‑1 (MQ=255)

                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                    <  1:1576841/93‑1 (MQ=255)

                                                     gCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAg                                   >  2:2199444/1‑93 (MQ=255)

                                                     gCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAg                                   >  1:716064/1‑93 (MQ=255)

                                                     gCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAg                                   <  1:1960732/93‑1 (MQ=255)

                                                      gctgtGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGc                                  <  2:921836/89‑1 (MQ=255)

                                                      cTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGc                                  <  1:1974262/93‑1 (MQ=255)

                                                       tGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCg                                 >  1:4314656/1‑93 (MQ=255)

                                                       tGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCg                                 >  2:4437117/1‑93 (MQ=255)

                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                <  1:1507013/93‑1 (MQ=255)

                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                               <  1:2795882/93‑1 (MQ=255)

                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                               <  1:4535750/93‑1 (MQ=255)

                                                           gCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGc                             <  1:993661/93‑1 (MQ=255)

                                                           gCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGc                             >  2:2404563/1‑93 (MQ=255)

                                                            ccccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCTGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGcc                            <  1:2420439/93‑1 (MQ=255)

                                                             cccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                           <  2:2722919/93‑1 (MQ=255)

                                                             cccAGCTATTGGCGACCAGATTGACCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                           <  2:1880393/93‑1 (MQ=255)

                                                              ccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCg                          >  2:3483965/1‑93 (MQ=255)

                                                               cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                         <  1:4570417/93‑1 (MQ=255)

                                                               cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                         >  2:328036/1‑93 (MQ=255)

                                                                aGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCt                        >  1:1911639/1‑93 (MQ=255)

                                                                aGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCt                        <  1:3347078/93‑1 (MQ=255)

                                                                 gCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGTGATGCCCGCtt                       >  2:3554760/1‑93 (MQ=255)

                                                                 gCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCtt                       <  2:1205806/93‑1 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                     >  1:1391825/1‑93 (MQ=255)

                                                                     ttGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgcgc                   >  2:3873346/1‑93 (MQ=255)

                                                                     ttGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgcgc                   >  1:3933266/1‑93 (MQ=255)

                                                                      tGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCa                  <  1:3736794/93‑1 (MQ=255)

                                                                        gCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACg                >  2:2100058/1‑93 (MQ=255)

                                                                         cGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACTCCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGt               <  2:852678/93‑1 (MQ=255)

                                                                         cGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGt               <  2:2957082/93‑1 (MQ=255)

                                                                          gACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGtt              >  1:3931231/1‑93 (MQ=255)

                                                                            ccAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtct            <  2:1617123/93‑1 (MQ=255)

                                                                             cAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc           >  1:3802394/1‑93 (MQ=255)

                                                                             cAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc           <  2:3985082/93‑1 (MQ=255)

                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa         <  1:2447435/93‑1 (MQ=255)

                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa         <  1:2423733/93‑1 (MQ=255)

                                                                                aTTGACCCCCCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAt        <  1:3559982/93‑1 (MQ=255)

                                                                                   gATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttt     >  1:594894/1‑93 (MQ=255)

                                                                                   gATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCGTGCGCACGTTCTCCAtttt     >  1:594893/1‑93 (MQ=255)

                                                                                     tccctcccGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtct   >  1:48030/1‑93 (MQ=255)

                                                                                     tCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtct   >  1:4528507/1‑93 (MQ=255)

                                                                                     tCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtct   <  1:3991735/93‑1 (MQ=255)

                                                                                      tcgttCTGTCGTGACGCGGACGAACCCCCGCTCTTCCGCCGAACGGGGATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc  <  1:835893/90‑1 (MQ=255)

                                                                                      cccTCCCGTCGTGGCGCGGGCGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTTCGCACGTTCTCCATTTtctc  <  1:835894/93‑1 (MQ=255)

                                                                                      cccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc  >  2:2976891/1‑93 (MQ=255)

                                                                                      cccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc  <  1:2085455/93‑1 (MQ=255)

                                                                                      cccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc  <  2:3077953/93‑1 (MQ=255)

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTC  >  NC_000913/1758171‑1758349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`