BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,307	T→C	Q35R (CAG→CGG)	ydiC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,307	0	T	C	98.7%	457.4 / ‑2.9	148	Q35R (CAG→CGG)	ydiC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (81/65); total (82/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 157 total aligned reads displayed.

TGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCT  >  NC_000913/1762218‑1762396

tGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGgccgct                                                                                        <  1:1020299/93‑1 (MQ=255)

 gTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCtt                                                                                       <  1:3140223/93‑1 (MQ=255)

        tCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccacc                                                                                >  1:2495201/1‑93 (MQ=255)

        tCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccacc                                                                                >  1:939720/1‑93 (MQ=255)

        tCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGATTTGccacc                                                                                >  2:1787409/1‑93 (MQ=255)

         cGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccccca                                                                               <  2:3594252/93‑1 (MQ=255)

         cGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGccacca                                                                               >  2:4722759/1‑93 (MQ=255)

           cTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGc                                                                             >  1:393675/1‑93 (MQ=255)

            tAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCt                                                                            >  2:1008866/1‑93 (MQ=255)

              acacTGTCGAGCACATAGCCAAAGCCAGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCCCTTTGCCACCACCTcc                                                                          >  2:4039010/1‑92 (MQ=255)

               cacTGTCGAGGACATAGCCAAAGCCCGCGGAGCCCGTTTGCTTCACGCTTAAGCGCACGCCGACCATTCCCGGCCGCTTTGTCACCAGCTCAc                                                                         <  2:2381446/93‑1 (MQ=255)

                acTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACg                                                                        >  2:745982/1‑93 (MQ=255)

                acTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACg                                                                        >  2:37333/1‑93 (MQ=255)

                acTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACg                                                                        <  1:2760632/93‑1 (MQ=255)

                 cTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACgg                                                                       >  2:4380214/1‑93 (MQ=255)

                 cTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACgg                                                                       >  2:3940327/1‑93 (MQ=255)

                     cGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACTCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAttt                                                                   >  1:1452911/1‑91 (MQ=255)

                     cGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtgt                                                                   >  1:71466/1‑93 (MQ=255)

                     cGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtgt                                                                   <  1:2430488/93‑1 (MQ=255)

                     cGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGAtgt                                                                   >  1:1452912/1‑93 (MQ=255)

                        gCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTa                                                                >  2:347518/1‑93 (MQ=255)

                         cacaTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTAt                                                               <  2:2245661/93‑1 (MQ=255)

                           caTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTTCCACCAGCTCACGGATGTGTATcg                                                             >  2:4639895/1‑93 (MQ=255)

                           caTAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcc                                                             >  1:3618192/1‑92 (MQ=255)

                             tAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgcc                                                           >  1:2622843/1‑93 (MQ=255)

                             tAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgcc                                                           >  1:1700339/1‑93 (MQ=255)

                             tAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgcc                                                           >  1:1246364/1‑93 (MQ=255)

                              aGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgccg                                                          >  2:2324712/1‑93 (MQ=255)

                              aGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgccg                                                          >  1:4596640/1‑93 (MQ=255)

                               gCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATcgccgc                                                         <  1:2320790/93‑1 (MQ=255)

                                 cAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCtc                                                       >  1:4050874/1‑92 (MQ=255)

                                   aaGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCg                                                     >  2:161634/1‑93 (MQ=255)

                                    aGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCgg                                                    <  1:809572/93‑1 (MQ=255)

                                     gCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCggg                                                   >  2:3244405/1‑93 (MQ=255)

                                      cccGCGCAGCCCGTTTTCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgt                                                  <  1:1094579/93‑1 (MQ=255)

                                        cgcgCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGgtgt                                                >  2:2901773/1‑93 (MQ=255)

                                         gcgcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGGGTc                                               >  1:2453359/1‑93 (MQ=255)

                                         gcgcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCAGCTGCGGGTGTc                                               >  1:1676134/1‑93 (MQ=255)

                                           gcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAg                                             >  2:2952225/1‑93 (MQ=255)

                                           gcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAg                                             <  1:2386463/93‑1 (MQ=255)

                                           gcAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAg                                             >  1:3056508/1‑93 (MQ=255)

                                            cAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGc                                            <  1:4658103/93‑1 (MQ=255)

                                            cAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGc                                            >  2:2322541/1‑93 (MQ=255)

                                             aGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCg                                           >  1:1048546/1‑93 (MQ=255)

                                             aGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCg                                           <  2:3577238/93‑1 (MQ=255)

                                             aGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCg                                           >  2:724069/1‑93 (MQ=255)

                                              gCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGt                                          <  2:622974/93‑1 (MQ=255)

                                              gCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGt                                          <  2:1170960/93‑1 (MQ=255)

                                              gCCCGTTTGCTTCACGCCTAAGCGAACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATTGCCGCTTCGCGTGCCTGCGt                                          >  2:3905201/1‑93 (MQ=255)

                                               cccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGtt                                         <  1:1510887/93‑1 (MQ=255)

                                               cccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGtt                                         >  1:2433170/1‑93 (MQ=255)

                                                ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        >  1:3113279/1‑93 (MQ=255)

                                                ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        >  2:2227618/1‑93 (MQ=255)

                                                ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        <  1:1007519/93‑1 (MQ=255)

                                                ccGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTa                                        >  1:3328640/1‑93 (MQ=255)

                                                ccGTTTGATTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGCGTCAGCGTTa                                        >  1:95641/1‑93 (MQ=255)

                                                  gTTTGCTTCACGCCTAAGCGCACGCCTACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAg                                      <  2:2920815/93‑1 (MQ=255)

                                                  gTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAg                                      <  2:4615253/93‑1 (MQ=255)

                                                   tttGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGGTAAGc                                     >  1:4207550/1‑93 (MQ=255)

                                                    ttGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGcc                                    >  2:1520466/1‑93 (MQ=255)

                                                     tGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCt                                   <  2:3068379/93‑1 (MQ=255)

                                                      gCTTCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCtt                                  <  2:365368/93‑1 (MQ=255)

                                                        ttCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGc                                <  1:1749511/93‑1 (MQ=255)

                                                        ttCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGc                                >  2:2396750/1‑93 (MQ=255)

                                                        ttCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGc                                <  1:3473567/93‑1 (MQ=255)

                                                         tCACGCCTCGGCGCCCGCCGCCCATACCCGGCCGCTTTGCCCCCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGcc                               <  1:3616001/93‑1 (MQ=255)

                                                         tCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGcc                               >  2:4024543/1‑93 (MQ=255)

                                                         tCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGcc                               <  1:4293686/93‑1 (MQ=255)

                                                         tCACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGcc                               <  1:3276509/93‑1 (MQ=255)

                                                          cACGCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCa                              <  1:4618960/93‑1 (MQ=255)

                                                             gCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGc                           <  1:3051873/93‑1 (MQ=255)

                                                             gCCTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGc                           >  1:3777007/1‑93 (MQ=255)

                                                              ccTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCg                          >  1:4427210/1‑93 (MQ=255)

                                                              ccTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCg                          >  2:1548772/1‑93 (MQ=255)

                                                               cTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGa                         >  1:1154846/1‑93 (MQ=255)

                                                               cTAAGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGa                         >  1:3831696/1‑93 (MQ=255)

                                                                  aGCGCACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAAt                      <  1:1747880/93‑1 (MQ=255)

                                                                   gcgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGGCAGTGTTAAGCCTTGCCAGGCGAAAtt                     >  2:3394200/1‑92 (MQ=255)

                                                                    cgcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCt                    <  2:393675/93‑1 (MQ=255)

                                                                     gcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCtt                   <  2:4209915/93‑1 (MQ=255)

                                                                     gcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCtt                   <  2:4650671/93‑1 (MQ=255)

                                                                     gcACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCtt                   <  1:913895/93‑1 (MQ=255)

                                                                      cACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtg                  >  2:3187080/1‑93 (MQ=255)

                                                                      cACGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtg                  <  1:3384759/93‑1 (MQ=255)

                                                                       aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 >  2:2609404/1‑93 (MQ=255)

                                                                       aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 <  1:486592/93‑1 (MQ=255)

                                                                       aCGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgt                 >  1:4406967/1‑93 (MQ=255)

                                                                        cGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgtg                <  1:1898528/93‑1 (MQ=255)

                                                                        cGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTtgtg                <  2:1388473/93‑1 (MQ=255)

                                                                        cGCCGACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCCtgtg                >  2:2168001/1‑93 (MQ=255)

                                                                            gACCATACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGtt            >  1:73540/1‑93 (MQ=255)

                                                                                aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAg        >  1:2527590/1‑93 (MQ=255)

                                                                                aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAg        >  2:842557/1‑93 (MQ=255)

                                                                                aTACCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAg        >  2:1184804/1‑93 (MQ=255)

                                                                                 atcccGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAgg       <  1:3694964/91‑1 (MQ=255)

                                                                                  aCCCGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGt      <  2:3561335/93‑1 (MQ=255)

                                                                                     cGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTcc   <  1:3303620/93‑1 (MQ=255)

                                                                                     cGGCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTcc   <  1:4488779/93‑1 (MQ=255)

                                                                                      ggCCGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCt  <  2:1926120/93‑1 (MQ=255)

                                                                                      ggCCGCCTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCt  <  2:1926121/93‑1 (MQ=255)

TGTCCGGCTCGCTAACACTGTCGAGCACATAGCCAAAGCCCGCGCAGCCCGTTTGCTTCACGCCTAAGCGCACGCCGACCATACCCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCT  >  NC_000913/1762218‑1762396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`