BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,191	T→C	R325R (CGT→CGC)	b1695 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,191	0	T	C	99.3%	442.3 / ‑4.4	140	R325R (CGT→CGC)	b1695	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (70/69); total (71/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 147 total aligned reads displayed.

GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280

 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        >  2:1848595/1‑93 (MQ=255)

 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        <  2:1829512/93‑1 (MQ=255)

  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGGACGCACCg                                                                                       >  2:1828507/1‑93 (MQ=255)

  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       >  1:3900612/1‑93 (MQ=255)

  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       >  1:3837315/1‑93 (MQ=255)

    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:1292667/1‑93 (MQ=255)

    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  2:1768767/1‑93 (MQ=255)

    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:3299042/1‑93 (MQ=255)

     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    <  1:113678/93‑1 (MQ=255)

     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    >  2:3528224/1‑93 (MQ=255)

      cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAt                                                                                   <  1:1843205/93‑1 (MQ=255)

       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  >  2:1889216/1‑93 (MQ=255)

       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  >  1:2154334/1‑93 (MQ=255)

       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  2:1334973/93‑1 (MQ=255)

       aTGGTGCTGAAAGTGGCATGACAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  1:1933100/93‑1 (MQ=255)

        tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:3741987/93‑1 (MQ=255)

         ggTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGa                                                                                >  2:1918331/1‑93 (MQ=255)

          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               >  2:4387822/1‑93 (MQ=255)

          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  2:3064306/93‑1 (MQ=255)

          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               >  1:301288/1‑93 (MQ=255)

            gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             >  2:3794162/1‑93 (MQ=255)

             cTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTc                                                                            <  1:646258/93‑1 (MQ=255)

              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           >  1:4632804/1‑93 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          >  1:4071293/1‑93 (MQ=255)

               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:3500158/93‑1 (MQ=255)

                aaaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:319247/93‑1 (MQ=255)

                aaaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         >  1:3106366/1‑93 (MQ=255)

                aaaGTGGCATGGCAAGCCGATCAGCAGCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  1:3766872/93‑1 (MQ=255)

                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:2504172/93‑1 (MQ=255)

                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:3289579/93‑1 (MQ=255)

                     ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:3762710/93‑1 (MQ=255)

                     ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:651194/93‑1 (MQ=255)

                      gcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  2:3889188/93‑1 (MQ=255)

                       catggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGatgatg                                                                  <  1:2002679/93‑1 (MQ=255)

                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 >  1:1910151/1‑93 (MQ=255)

                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 >  1:4623882/1‑93 (MQ=255)

                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:4675393/1‑93 (MQ=255)

                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  1:2687587/1‑93 (MQ=255)

                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAACTCATTGATGATGCg                                                                >  2:2163837/1‑93 (MQ=255)

                         tcgcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                <  2:13061/91‑1 (MQ=255)

                          ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  1:909223/1‑93 (MQ=255)

                          ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:4514105/93‑1 (MQ=255)

                           gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              <  2:2414247/93‑1 (MQ=255)

                           gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              >  1:4746746/1‑93 (MQ=255)

                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  1:8015/1‑93 (MQ=255)

                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  2:417820/1‑93 (MQ=255)

                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         <  2:4365683/93‑1 (MQ=255)

                                  gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  2:2428403/1‑93 (MQ=255)

                                  gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATCGATGATGCGATTCAAATc                                                       <  1:165773/93‑1 (MQ=255)

                                   aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCa                                                      <  2:3382869/93‑1 (MQ=255)

                                   aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCa                                                      >  2:4697014/1‑93 (MQ=255)

                                    tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:620216/1‑93 (MQ=255)

                                        cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 <  1:2064572/93‑1 (MQ=255)

                                        cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 >  2:1748940/1‑93 (MQ=255)

                                         aTCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCATTGGAAGTCATTGATGATGCGATTCAAATCATGGGCg                                                <  2:2946463/93‑1 (MQ=255)

                                          tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:4016778/1‑93 (MQ=255)

                                           cAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGt                                              <  1:2616144/93‑1 (MQ=255)

                                            aGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTc                                             >  2:2743064/1‑93 (MQ=255)

                                            aGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTc                                             >  1:756851/1‑93 (MQ=255)

                                              tCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTg                                           <  1:1653545/93‑1 (MQ=255)

                                                aCTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTggg                                         >  1:2492415/1‑93 (MQ=255)

                                                 cTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTgggt                                        >  1:2704594/1‑92 (MQ=255)

                                                   gcgcACCAGCGCTGCGCTGGCAAAGCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      <  2:681007/93‑1 (MQ=255)

                                                   gcgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      <  2:681008/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  1:1164949/93‑1 (MQ=255)

                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  2:3507249/93‑1 (MQ=255)

                                                      cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   >  2:1843406/1‑93 (MQ=255)

                                                         cAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGa                                <  1:1494845/93‑1 (MQ=255)

                                                          aGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAt                               <  2:418651/93‑1 (MQ=255)

                                                          aGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAt                               >  1:4316510/1‑93 (MQ=255)

                                                           gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  2:4587908/93‑1 (MQ=255)

                                                             gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            <  1:3807607/93‑1 (MQ=255)

                                                             gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            >  2:2526301/1‑93 (MQ=255)

                                                              cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg                           >  2:4517502/1‑93 (MQ=255)

                                                              cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg                           >  1:616289/1‑93 (MQ=255)

                                                                  gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       >  2:2880153/1‑93 (MQ=255)

                                                                  gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  2:1287825/93‑1 (MQ=255)

                                                                   cTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc                      >  2:3404589/1‑93 (MQ=255)

                                                                      gCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtc                   <  2:62073/93‑1 (MQ=255)

                                                                         aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                >  1:3021447/1‑93 (MQ=255)

                                                                           actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              >  1:1198249/1‑93 (MQ=255)

                                                                           actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:2758890/93‑1 (MQ=255)

                                                                           actgtactgtGCACGCACCGCAAGGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              >  2:2307115/1‑93 (MQ=255)

                                                                            ctgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGc             <  1:1580661/93‑1 (MQ=255)

                                                                            ctgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGc             >  2:1027357/1‑93 (MQ=255)

                                                                             tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  2:3725775/1‑93 (MQ=255)

                                                                              gtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCtt           <  1:2512241/93‑1 (MQ=255)

                                                                              gtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCtt           >  1:3554546/1‑93 (MQ=255)

                                                                               tactgtGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  2:4515330/1‑93 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  1:4437879/93‑1 (MQ=255)

                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  2:2466971/93‑1 (MQ=255)

                                                                                actgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCt         >  1:3102106/1‑93 (MQ=255)

                                                                                 ctgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTg        <  2:515458/93‑1 (MQ=255)

                                                                                  tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       >  1:2743279/1‑93 (MQ=255)

                                                                                  tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       <  2:3246823/93‑1 (MQ=255)

                                                                                   gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  1:1057550/1‑93 (MQ=255)

                                                                                     gcacgcacCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt    >  1:4256886/1‑93 (MQ=255)

                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:3550392/1‑93 (MQ=255)

                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:4180356/1‑93 (MQ=255)

                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  2:2725715/1‑93 (MQ=255)

GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`