BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,298	A→G	K146K (AAA→AAG)	sppA →	protease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,298	0	A	G	99.3%	438.7 / NA	136	K146K (AAA→AAG)	sppA	protease IV
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (58/77); total (59/77)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGT  >  NC_000913/1847208‑1847387

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTGTGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        >  2:2257409/1‑93 (MQ=255)

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        <  2:793682/93‑1 (MQ=255)

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        >  2:2257410/1‑93 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      <  1:1361913/93‑1 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      >  2:3062596/1‑93 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      <  1:2214334/93‑1 (MQ=255)

    aTGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGtt                                                                                     <  1:687870/93‑1 (MQ=255)

     tGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGttt                                                                                    <  1:1464356/93‑1 (MQ=255)

      ggATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTa                                                                                   <  2:370090/93‑1 (MQ=255)

      gcagcTGAAAAGCTTCGCAGGCGGCGTCCAACCGTCGATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTa                                                                                   <  2:370089/89‑1 (MQ=255)

        aTCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATg                                                                                 <  2:1489838/93‑1 (MQ=255)

        aTCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATg                                                                                 >  1:138700/1‑93 (MQ=255)

          cTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGcc                                                                               <  2:2747078/93‑1 (MQ=255)

           tGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCg                                                                              >  2:975452/1‑93 (MQ=255)

            gAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGt                                                                             >  1:2563922/1‑93 (MQ=255)

              aaaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTg                                                                           <  1:3572373/93‑1 (MQ=255)

              aaaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTg                                                                           <  2:3865338/93‑1 (MQ=255)

               aaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTgg                                                                          >  1:1919008/1‑93 (MQ=255)

               aaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGGTgg                                                                          >  1:4298950/1‑93 (MQ=255)

                aaCTTCGCAGGCGGAGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGAGGGAAGCCGGTTTATGCCGTTGGc                                                                         <  2:2533799/93‑1 (MQ=255)

                  cTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCga                                                                       <  2:4631955/93‑1 (MQ=255)

                   ttCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCgag                                                                      >  2:1615261/1‑93 (MQ=255)

                      gCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAAc                                                                   <  1:3873562/93‑1 (MQ=255)

                      gCAGGCGGCGACCAACCATCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAAc                                                                   <  1:4146637/93‑1 (MQ=255)

                       cAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACt                                                                  >  1:2782046/1‑93 (MQ=255)

                         ggcggcggcCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  1:3300036/93‑1 (MQ=255)

                         ggcggcGACCACCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  2:4415734/93‑1 (MQ=255)

                         ggcggcGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  1:2063228/93‑1 (MQ=255)

                          gcggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACa                                                               >  2:3591933/1‑93 (MQ=255)

                          gcggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACa                                                               <  2:1169265/93‑1 (MQ=255)

                           cggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAg                                                              <  1:609451/93‑1 (MQ=255)

                            ggcgACCAACCGTCTATGCAGTACATCGGCAAAGGTCTGAAAAAGTTTAGTGACAGAGGGAAACCGGTTTATGCCATTGGCTAGAACTACAGc                                                             >  2:2523798/1‑93 (MQ=255)

                            ggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGc                                                             <  1:4559781/93‑1 (MQ=255)

                             gcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGcc                                                            <  1:3213142/93‑1 (MQ=255)

                                aCCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAgg                                                         <  2:1125356/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:2131383/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        >  2:155246/1‑93 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:1882838/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:2055281/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCAGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        >  1:1658568/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      >  2:822651/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      >  2:3877901/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      <  2:98048/93‑1 (MQ=255)

                                     ccGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCaa                                                    <  2:2468187/93‑1 (MQ=255)

                                       gTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAata                                                  <  1:1084096/93‑1 (MQ=255)

                                       gTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAata                                                  <  1:1398276/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 <  1:1764918/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 >  2:736056/1‑93 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 <  2:1376029/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAAAGCCAGGGGCAatat                                                 >  2:283512/1‑93 (MQ=255)

                                          tATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAAtatta                                               <  2:546400/93‑1 (MQ=255)

                                          tATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAAtatta                                               <  1:3959066/93‑1 (MQ=255)

                                            tGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtc                                             <  1:3562913/93‑1 (MQ=255)

                                             gCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtct                                            <  1:85251/93‑1 (MQ=255)

                                             gCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtct                                            <  2:407950/93‑1 (MQ=255)

                                              cAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtctc                                           <  1:3220717/93‑1 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         >  1:1348719/1‑93 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         <  1:3628463/93‑1 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         >  1:1106284/1‑93 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  2:1087020/93‑1 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  1:621134/93‑1 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  1:3365611/93‑1 (MQ=255)

                                                  aCATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCa                                       <  2:1345289/93‑1 (MQ=255)

                                                  aCATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCa                                       <  2:4345901/93‑1 (MQ=255)

                                                   cATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAg                                      <  2:4552465/93‑1 (MQ=255)

                                                    aTCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGt                                     >  1:1620471/1‑93 (MQ=255)

                                                       ggCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGTGGCAATATTATCTCGCCAGTTTc                                  >  2:3393872/1‑93 (MQ=255)

                                                        gCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCg                                 >  2:2127155/1‑93 (MQ=255)

                                                           aaGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCa                              <  2:3218616/93‑1 (MQ=255)

                                                           aaGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCa                              >  1:2359857/1‑93 (MQ=255)

                                                            aGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCaa                             >  1:2093232/1‑93 (MQ=255)

                                                            aGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCaa                             >  1:574285/1‑93 (MQ=255)

                                                             gCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAAt                            >  2:1153612/1‑93 (MQ=255)

                                                              ctctGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATa                           >  1:3408005/1‑93 (MQ=255)

                                                              ctctGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATa                           <  2:2059577/93‑1 (MQ=255)

                                                                 tGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGACGTTGGCGAGAACTACAGCCACGGGCAATATTATCTCGTCAGTTTCGCCAATaaaa                        >  2:255332/1‑93 (MQ=255)

                                                                   aaaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGGAATATTATATCGCAAGTTTGGCCAATAAAAtt                      >  1:1900535/1‑93 (MQ=255)

                                                                    aaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAAttt                     >  1:2559375/1‑93 (MQ=255)

                                                                    aaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAAttt                     <  1:3673710/93‑1 (MQ=255)

                                                                      gagTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTgg                   <  2:4527972/93‑1 (MQ=255)

                                                                        gTTTGGTGACAGCGGGAATGCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGCCAATATTATCTCGCCAGACTCGCCAATAAAATTTGGCt                 >  2:872450/1‑93 (MQ=255)

                                                                        gTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCt                 <  2:924448/93‑1 (MQ=255)

                                                                        gTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCt                 >  2:872449/1‑93 (MQ=255)

                                                                         tttCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTg                <  1:1158618/93‑1 (MQ=255)

                                                                         tttCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTg                >  2:4561195/1‑93 (MQ=255)

                                                                           tCGTGGCAACGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtc              <  2:2841101/93‑1 (MQ=255)

                                                                           tCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtc              <  1:4679467/93‑1 (MQ=255)

                                                                            cGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtct             >  1:366981/1‑93 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            <  2:1498222/93‑1 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            <  1:4283653/93‑1 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            >  2:2154747/1‑93 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            >  1:3011788/1‑93 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           <  2:1599049/93‑1 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           <  2:4660233/93‑1 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           >  2:1895453/1‑93 (MQ=255)

                                                                                  aGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCaa       >  1:1217244/1‑93 (MQ=255)

                                                                                  aGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCaa       <  1:4607139/93‑1 (MQ=255)

                                                                                     gggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGc    <  2:1143909/93‑1 (MQ=255)

                                                                                     gggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGc    <  1:1439836/93‑1 (MQ=255)

                                                                                      ggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCg   >  2:61658/1‑93 (MQ=255)

TGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGT  >  NC_000913/1847208‑1847387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`