BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,370	T→C	E28E (GAA→GAG)	yeaA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,370	0	T	C	100.0%	550.0 / NA	168	E28E (GAA→GAG)	yeaA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (91/77); total (91/77)

Only 100 of 175 total aligned reads displayed.

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

ggTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTc                                                                                        <  1:2787284/93‑1 (MQ=255)

  tttGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTg                                                                                      >  1:2537506/1‑93 (MQ=255)

      ggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTccc                                                                                  >  1:1600396/1‑93 (MQ=255)

         aTGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATg                                                                               <  1:4038524/93‑1 (MQ=255)

          tGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGa                                                                              >  1:1238764/1‑93 (MQ=255)

          agAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGa                                                                              <  1:4166459/92‑1 (MQ=255)

            aaaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGAtt                                                                            <  1:3725649/93‑1 (MQ=255)

            aaaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGAtt                                                                            >  1:444139/1‑93 (MQ=255)

             aaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTc                                                                           >  2:1288648/1‑93 (MQ=255)

              aCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCt                                                                          <  2:1034354/93‑1 (MQ=255)

                aGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGc                                                                        >  2:2773416/1‑93 (MQ=255)

                 gCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                       >  1:765137/1‑93 (MQ=255)

                   ggggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTc                                                                     <  2:2454096/93‑1 (MQ=255)

                   ggggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTc                                                                     >  1:2373882/1‑93 (MQ=255)

                   ggggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACCACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTc                                                                     >  2:2709326/1‑93 (MQ=255)

                    gggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCa                                                                    <  2:3971867/93‑1 (MQ=255)

                    gggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCa                                                                    >  2:3547704/1‑93 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  1:2069563/1‑93 (MQ=255)

                         tCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                                               >  1:3352052/1‑93 (MQ=255)

                         tCGCAGATCAAACAGTGATATACGCCGCCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                                               >  2:2914922/1‑93 (MQ=255)

                           gCAGATCAAACAGTGATATACCGCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaaa                                                             <  1:1332575/93‑1 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            <  2:357890/93‑1 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            >  2:4153356/1‑93 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            <  1:293564/93‑1 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            >  2:4661518/1‑93 (MQ=255)

                             aGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACt                                                           >  2:3278790/1‑93 (MQ=255)

                              gATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTg                                                          >  1:2388581/1‑93 (MQ=255)

                               aTCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                         >  1:1265604/1‑93 (MQ=255)

                                tCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCa                                                        >  1:2605243/1‑93 (MQ=255)

                                tCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCa                                                        >  2:2819260/1‑93 (MQ=255)

                                 cAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAt                                                       >  1:2249138/1‑93 (MQ=255)

                                  aaaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtc                                                      <  1:911556/93‑1 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     >  1:695545/1‑93 (MQ=255)

                                    aCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                                    <  1:1733171/93‑1 (MQ=255)

                                     cAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                                   >  2:2131521/1‑93 (MQ=255)

                                       atGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 <  1:1226999/92‑1 (MQ=255)

                                        tGATATACGCCGCCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCCTCTCGGAc                                                >  2:936129/1‑93 (MQ=255)

                                           tataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaaa                                             <  1:3758104/93‑1 (MQ=255)

                                            ataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAt                                            >  2:462408/1‑93 (MQ=255)

                                                gCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttttt                                        >  2:4629117/1‑93 (MQ=255)

                                                 ccGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                       <  1:1149866/93‑1 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     <  1:3555783/93‑1 (MQ=255)

                                                    tCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                                    >  2:2850031/1‑93 (MQ=255)

                                                    tCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                                    >  1:50637/1‑93 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   <  1:3348797/93‑1 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   <  2:3935449/93‑1 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   >  1:75117/1‑93 (MQ=255)

                                                      aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAg                                  >  2:2665837/1‑93 (MQ=255)

                                                      aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAg                                  >  2:406550/1‑93 (MQ=255)

                                                       cGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGt                                 >  1:3965770/1‑93 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                >  2:1378741/1‑93 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                <  2:3745962/93‑1 (MQ=255)

                                                         cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc                               >  2:2400992/1‑93 (MQ=255)

                                                          ttGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttct                              >  1:3491361/1‑93 (MQ=255)

                                                           tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttctt                             <  1:2475204/93‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  1:1032997/93‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  1:1604760/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  2:1564144/93‑1 (MQ=255)

                                                              tATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttctc                          >  2:1452798/1‑92 (MQ=255)

                                                               aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGc                         <  2:3712652/93‑1 (MQ=255)

                                                               aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGc                         >  2:4579067/1‑93 (MQ=255)

                                                               aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGc                         >  1:4529342/1‑93 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        >  2:3727438/1‑93 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        <  1:2633598/93‑1 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        <  1:1611342/93‑1 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        <  2:1947855/93‑1 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     <  1:2932524/93‑1 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     <  1:3838361/93‑1 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     <  1:4348853/93‑1 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAg                    >  1:4026503/1‑93 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCAAGTAAAACTGCATCTCGGACCAATTTTTTTTCAGTTCTTCTGCCGAAg                    >  1:4256823/1‑93 (MQ=255)

                                                                     tAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAgg                   >  1:3090037/1‑93 (MQ=255)

                                                                      aaaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGt                  <  2:2919342/93‑1 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGTCAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  2:3402459/93‑1 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  1:1020596/93‑1 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  2:2009864/93‑1 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  2:3374576/93‑1 (MQ=255)

                                                                        aCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGttt                <  1:4547035/93‑1 (MQ=255)

                                                                        aCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGttt                >  1:2078503/1‑93 (MQ=255)

                                                                          gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGGAGGTttat              >  1:2037121/1‑93 (MQ=255)

                                                                          gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttat              >  1:2859426/1‑93 (MQ=255)

                                                                            cccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttatta            >  2:2264711/1‑93 (MQ=255)

                                                                            cccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttatta            <  2:1145312/93‑1 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           >  1:1992321/1‑93 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           >  1:134034/1‑93 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCGGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           <  1:1917795/93‑1 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          >  1:2364504/1‑93 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          <  2:2621445/93‑1 (MQ=255)

                                                                               gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGcc         <  2:4245683/93‑1 (MQ=255)

                                                                                tAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCa        >  2:3113794/1‑93 (MQ=255)

                                                                                tAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCa        >  1:57831/1‑93 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      <  1:4289700/93‑1 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      >  2:4404500/1‑93 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      <  2:1039044/93‑1 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      >  2:3192230/1‑93 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      <  1:3187266/93‑1 (MQ=255)

                                                                                   aTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAttt     <  1:416240/93‑1 (MQ=255)

                                                                                   aTGGCGGCTATGTCCCATGATTCTGCATCACGTAAACCTGCATCTCGGACAAATTTTTTTTCAGTTATTCTACCGAAGGTATATTAGCCAttt     <  2:4220313/93‑1 (MQ=255)

                                                                                    tGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTg    >  1:1854686/1‑93 (MQ=255)

                                                                                    tGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTg    <  2:1121477/93‑1 (MQ=255)

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`