BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,362	T→C	V144A (GTC→GCC)	yeaG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,362	0	T	C	99.3%	451.7 / NA	142	V144A (GTC→GCC)	yeaG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (68/73); minor base A (0/1); total (68/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 146 total aligned reads displayed.

GGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCA  >  NC_000913/1865273‑1865451

 ggTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAAc                                                                                       <  2:3922289/93‑1 (MQ=255)

 ggTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGACCGGCCAAc                                                                                       >  1:3841387/1‑93 (MQ=255)

 ggTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGACCGGCCAAc                                                                                       >  1:3841388/1‑93 (MQ=255)

  gTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACg                                                                                      >  1:2972131/1‑93 (MQ=255)

   tAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGa                                                                                     >  2:2500362/1‑93 (MQ=255)

    aaaTCATCGCTTGCGGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGat                                                                                    <  1:1751597/93‑1 (MQ=255)

     aaTCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGatc                                                                                   <  1:3514315/93‑1 (MQ=255)

      atcatcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGatca                                                                                  <  2:2053269/93‑1 (MQ=255)

      atcatcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGatca                                                                                  >  1:4125073/1‑93 (MQ=255)

        catcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGatcatc                                                                                <  1:994597/93‑1 (MQ=255)

         atcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATcc                                                                               <  1:4506826/93‑1 (MQ=255)

          tcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCg                                                                              >  2:1548291/1‑93 (MQ=255)

            gCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGtt                                                                            <  1:3360169/93‑1 (MQ=255)

            gCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGtt                                                                            >  1:2371640/1‑93 (MQ=255)

             cTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTc                                                                           >  1:3375224/1‑93 (MQ=255)

                  tGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCt                                                                      >  1:1196436/1‑93 (MQ=255)

                   gAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCtt                                                                     <  2:3287227/93‑1 (MQ=255)

                    aGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCttt                                                                    >  1:2926093/1‑93 (MQ=255)

                        aCTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCaa                                                                >  1:1847289/1‑93 (MQ=255)

                        aCTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCaa                                                                <  2:815056/93‑1 (MQ=255)

                        aCTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCaa                                                                >  1:3767887/1‑93 (MQ=255)

                          tGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAATGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATc                                                              <  2:1285742/93‑1 (MQ=255)

                          tGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATc                                                              <  1:311908/93‑1 (MQ=255)

                          tGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATc                                                              <  1:311907/93‑1 (MQ=255)

                           gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATcc                                                             <  2:2704334/93‑1 (MQ=255)

                           gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATcc                                                             <  1:4335460/93‑1 (MQ=255)

                           gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATcc                                                             >  2:2331493/1‑93 (MQ=255)

                           gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATcc                                                             <  1:590610/93‑1 (MQ=255)

                           gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATcc                                                             >  1:548692/1‑93 (MQ=255)

                            aaaTCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCg                                                            >  2:4326114/1‑93 (MQ=255)

                              aTCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCa                                                          <  1:1689609/93‑1 (MQ=255)

                              aTCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCa                                                          >  2:677090/1‑93 (MQ=255)

                              aTCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCa                                                          <  1:2741932/93‑1 (MQ=255)

                               tCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAg                                                         <  2:864999/93‑1 (MQ=255)

                                cATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAgg                                                        >  2:4251396/1‑93 (MQ=255)

                                 aTTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGa                                                       >  2:2015198/1‑93 (MQ=255)

                                 aTTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGa                                                       <  1:2873341/93‑1 (MQ=255)

                                 aTTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGa                                                       >  2:4215471/1‑93 (MQ=255)

                                  ttAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGaa                                                      <  1:113790/93‑1 (MQ=255)

                                   tAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAg                                                     <  2:543588/93‑1 (MQ=255)

                                   tAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAg                                                     >  1:1512489/1‑93 (MQ=255)

                                    aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGa                                                    <  1:2586619/93‑1 (MQ=255)

                                    aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGa                                                    <  1:495817/93‑1 (MQ=255)

                                    aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGa                                                    <  1:495816/93‑1 (MQ=255)

                                     aTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGAt                                                   <  1:3186742/93‑1 (MQ=255)

                                      tGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATg                                                  >  2:2806131/1‑93 (MQ=255)

                                      tGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATg                                                  <  2:3452377/93‑1 (MQ=255)

                                       ggaGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgc                                                 <  1:1444527/91‑1 (MQ=255)

                                       gCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgc                                                 >  2:528093/1‑93 (MQ=255)

                                         aGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgcgc                                               >  2:4655202/1‑93 (MQ=255)

                                          gCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCa                                              <  2:20146/93‑1 (MQ=255)

                                           cTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAg                                             <  1:1852619/93‑1 (MQ=255)

                                            tCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGa                                            >  2:3326944/1‑93 (MQ=255)

                                                aCCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCt                                        <  1:2489996/93‑1 (MQ=255)

                                                aCCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCt                                        <  1:2489995/93‑1 (MQ=255)

                                                 ccGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTg                                       >  1:4548735/1‑93 (MQ=255)

                                                 ccGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTg                                       >  2:4568747/1‑93 (MQ=255)

                                                   gATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGa                                     <  1:4666903/93‑1 (MQ=255)

                                                     tttATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTATTTTCAATACGCAGGAAGATGCGCAGATTCTGGaaa                                   >  2:2552030/1‑93 (MQ=255)

                                                      ttATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGaaaa                                  >  1:2994872/1‑93 (MQ=255)

                                                       tATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGaaaaa                                 <  1:1060436/93‑1 (MQ=255)

                                                         tGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAaga                               <  1:3440678/93‑1 (MQ=255)

                                                         tGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAaga                               <  1:421470/93‑1 (MQ=255)

                                                         tGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAaga                               <  1:52784/93‑1 (MQ=255)

                                                         tGTATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAaga                               <  1:1977854/93‑1 (MQ=255)

                                                           tATTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGt                             <  1:3036309/93‑1 (MQ=255)

                                                            ttagAGCGCGAACGGTGAGCGTAGCTCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTa                            <  1:2302129/90‑1 (MQ=255)

                                                            aTTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTa                            <  2:4285660/93‑1 (MQ=255)

                                                            aTTGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTa                            <  1:1770685/93‑1 (MQ=255)

                                                             ttGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGCTTCTGGAAAAAGAGTAt                           >  2:3744911/1‑93 (MQ=255)

                                                             ttGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTAt                           >  2:2059831/1‑93 (MQ=255)

                                                             ttGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTAt                           <  2:4059906/93‑1 (MQ=255)

                                                             ttGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTAt                           >  1:4114639/1‑93 (MQ=255)

                                                              tGAGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATg                          <  2:2166566/93‑1 (MQ=255)

                                                                aGCGCGATCGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTATTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGc                        <  1:1595022/93‑1 (MQ=255)

                                                                aGCGCGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGc                        >  1:3661247/1‑93 (MQ=255)

                                                                 gcgcGAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGct                       >  1:423315/1‑92 (MQ=255)

                                                                   gcgAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCAtt                     >  2:2841152/1‑93 (MQ=255)

                                                                   gcgAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCAtt                     >  2:710163/1‑93 (MQ=255)

                                                                    cgAACGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTc                    >  1:2900123/1‑93 (MQ=255)

                                                                     gAACGGTGAGCGTAGCCCGGACAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTcc                   <  1:2234236/93‑1 (MQ=255)

                                                                      aaCGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCt                  >  2:279075/1‑93 (MQ=255)

                                                                       aCGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTc                 <  2:4361964/93‑1 (MQ=255)

                                                                       aCGGTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTc                 >  2:1566035/1‑93 (MQ=255)

                                                                         ggTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGc               <  1:3220304/93‑1 (MQ=255)

                                                                          gTGAGCGTAGCCGGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGcc              <  1:2053191/93‑1 (MQ=255)

                                                                          gTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGcc              >  1:2604149/1‑93 (MQ=255)

                                                                          gTGAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGcc              >  2:177786/1‑93 (MQ=255)

                                                                            gAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGt            <  1:3598858/93‑1 (MQ=255)

                                                                            gAGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGt            >  2:3479383/1‑93 (MQ=255)

                                                                             aGCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt           <  2:291812/93‑1 (MQ=255)

                                                                              gCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTa          <  1:1306389/93‑1 (MQ=255)

                                                                              gCGTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTa          <  2:83767/93‑1 (MQ=255)

                                                                                gTAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTAtc        <  1:1134141/93‑1 (MQ=255)

                                                                                 tAGCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGGATGGCATTCCTCGCCGTTAtct       >  2:1127948/1‑93 (MQ=255)

                                                                                   gCCCGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCg     <  2:3519780/93‑1 (MQ=255)

                                                                                     ccGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGc   <  2:1854529/93‑1 (MQ=255)

                                                                                     ccGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGc   >  2:1819416/1‑93 (MQ=255)

                                                                                     ccGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGc   <  2:1261656/93‑1 (MQ=255)

                                                                                      cGGCCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCa  >  2:873621/1‑93 (MQ=255)

GGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCA  >  NC_000913/1865273‑1865451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`