BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,871,984	A→G	E129E (GAA→GAG)	yeaK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,871,984	0	A	G	96.9%	603.6 / 5.3	196	E129E (GAA→GAG)	yeaK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (4/2); new base G (138/52); total (142/54)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 203 total aligned reads displayed.

GATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGA  >  NC_000913/1871895‑1872073

gATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGa                                                                                        >  1:3552001/1‑93 (MQ=255)

gATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGa                                                                                        >  2:3179847/1‑93 (MQ=255)

 aTGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGCGCGAt                                                                                       >  2:1374241/1‑93 (MQ=255)

 aTGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGAt                                                                                       >  1:2370835/1‑93 (MQ=255)

 aTGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGAt                                                                                       >  2:3822477/1‑93 (MQ=255)

  tGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGAtt                                                                                      >  2:4137326/1‑93 (MQ=255)

     aCTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtgt                                                                                   >  1:1349485/1‑92 (MQ=255)

     aCTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtga                                                                                   >  2:1178197/1‑93 (MQ=255)

       tCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtgatg                                                                                 >  2:4567608/1‑93 (MQ=255)

       tCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtgatg                                                                                 >  2:2531810/1‑93 (MQ=255)

       tCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtgatg                                                                                 >  2:2043456/1‑93 (MQ=255)

       tCACAGGCTGTGTCTTCGGCGCGATACCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCCAGCGCTTtgatg                                                                                 >  2:195685/1‑93 (MQ=255)

        cacaGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTtgatga                                                                                >  2:3843738/1‑93 (MQ=255)

         acaGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGaa                                                                               <  1:83693/93‑1 (MQ=255)

         acaGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGaa                                                                               >  1:953414/1‑93 (MQ=255)

           aGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAAt                                                                             >  1:2126798/1‑93 (MQ=255)

            ggCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATc                                                                            >  1:4282687/1‑93 (MQ=255)

              cTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGc                                                                          <  1:3875606/93‑1 (MQ=255)

              cTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGc                                                                          <  1:2771191/93‑1 (MQ=255)

               tgtgTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGcc                                                                         >  1:4696958/1‑93 (MQ=255)

               tgtgTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGcc                                                                         >  2:2180127/1‑93 (MQ=255)

                gtgtCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCt                                                                        >  1:1518883/1‑93 (MQ=255)

                 tgtCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCtt                                                                       >  1:3099628/1‑93 (MQ=255)

                    cTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCaa                                                                    <  2:1431321/93‑1 (MQ=255)

                     ttCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAAt                                                                   <  1:3766263/93‑1 (MQ=255)

                     ttCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAAt                                                                   <  1:3660809/93‑1 (MQ=255)

                     ttCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAAt                                                                   >  1:3410738/1‑93 (MQ=255)

                         gcgcGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAg                                                               <  1:4125898/93‑1 (MQ=255)

                           gcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGc                                                             >  1:3498038/1‑93 (MQ=255)

                           ccgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGc                                                             <  2:1713758/92‑1 (MQ=255)

                            cgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCa                                                            <  2:191304/93‑1 (MQ=255)

                            cgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCa                                                            >  1:2841982/1‑93 (MQ=255)

                             gaaccccccTGTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCAt                                                           <  2:3242391/90‑1 (MQ=255)

                             gATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCAt                                                           >  2:3116787/1‑93 (MQ=255)

                               tCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGc                                                         >  1:3290274/1‑93 (MQ=255)

                               tCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGc                                                         >  1:728521/1‑93 (MQ=255)

                                ccccccTTTCAGCTTCCATCCAAAACTCACACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAACTCGCCTTCAATGCAGGCATGCt                                                        >  1:3940243/1‑93 (MQ=255)

                                 cccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGTCCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTg                                                       <  1:1928992/93‑1 (MQ=255)

                                 cccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGATCGATTTGATGAAATGGCCTCCAATGCAGGCATGCTg                                                       >  1:4717342/1‑93 (MQ=255)

                                 cccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTg                                                       >  2:3990396/1‑93 (MQ=255)

                                  ccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTgg                                                      >  2:72946/1‑93 (MQ=255)

                                  ccccTTTCAGCTACCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCTTGCGAATTGATGAAATCGCCTTCAATGCAGGCATGCTgg                                                      >  2:4210672/1‑93 (MQ=255)

                                    ccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGAt                                                    >  2:4109203/1‑93 (MQ=255)

                                    ccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGAt                                                    >  2:1064864/1‑93 (MQ=255)

                                    ccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGAt                                                    >  2:1041346/1‑93 (MQ=255)

                                     cTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATa                                                   <  1:2467124/93‑1 (MQ=255)

                                     cTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATa                                                   <  2:542546/93‑1 (MQ=255)

                                      tttCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATaa                                                  >  1:1849845/1‑93 (MQ=255)

                                      tttCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATaa                                                  >  1:2896242/1‑93 (MQ=255)

                                         cAGCTCCCACCCAAAACTCAAACTGGTCTCCGACCCTCTCCTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATc                                               <  1:2498522/93‑1 (MQ=255)

                                          aGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATcc                                              >  2:3615199/1‑93 (MQ=255)

                                               ccatccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAt                                         <  2:3410192/93‑1 (MQ=255)

                                                catccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATACGTTAtt                                        >  2:4454042/1‑93 (MQ=255)

                                                 atccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAttt                                       >  2:3514298/1‑93 (MQ=255)

                                                  tccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAtttt                                      >  1:2218824/1‑93 (MQ=255)

                                                  tccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAtttt                                      >  2:4724110/1‑93 (MQ=255)

                                                  tccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAtttt                                      >  2:3903247/1‑93 (MQ=255)

                                                  tccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAtttt                                      >  2:650879/1‑93 (MQ=255)

                                                  tccaAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTAtttt                                      >  1:2745658/1‑93 (MQ=255)

                                                    caAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGa                                    <  1:900955/93‑1 (MQ=255)

                                                     aaaaCTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGaa                                   >  2:553410/1‑93 (MQ=255)

                                                      aaaCTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGaaa                                  >  1:3919929/1‑93 (MQ=255)

                                                        aCTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAAc                                <  2:1450070/93‑1 (MQ=255)

                                                        aCTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAAc                                >  2:3730172/1‑93 (MQ=255)

                                                         cTCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAAcc                               >  1:4422336/1‑93 (MQ=255)

                                                          tCAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcg                              >  1:978133/1‑93 (MQ=255)

                                                           cAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGCTGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGACAAcctg                             >  2:1211454/1‑91 (MQ=255)

                                                           cAAACTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgc                             >  2:1750228/1‑93 (MQ=255)

                                                            aaaCTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgcg                            >  2:2606071/1‑93 (MQ=255)

                                                              aCTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGa                          >  1:1628239/1‑93 (MQ=255)

                                                               cTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat                         <  2:3155068/93‑1 (MQ=255)

                                                               cTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat                         >  2:1505855/1‑93 (MQ=255)

                                                               cTGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat                         >  2:2726781/1‑93 (MQ=255)

                                                                tGGTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGatt                        <  1:1463714/93‑1 (MQ=255)

                                                                  gTTGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGattat                      >  1:4579102/1‑93 (MQ=255)

                                                                   ttGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGattatt                     >  1:1983965/1‑93 (MQ=255)

                                                                   ttGCCGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGattatt                     <  2:3341474/93‑1 (MQ=255)

                                                                       cGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcg                 >  2:2329995/1‑93 (MQ=255)

                                                                       cGACCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcg                 >  1:127901/1‑93 (MQ=255)

                                                                         aCCCTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgca               >  1:2873126/1‑93 (MQ=255)

                                                                          cccTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcat              >  2:2742094/1‑93 (MQ=255)

                                                                          cccTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcat              >  2:1008324/1‑93 (MQ=255)

                                                                           ccTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcatt             <  1:1735520/93‑1 (MQ=255)

                                                                           ccTCTACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcatt             >  1:641749/1‑93 (MQ=255)

                                                                            ctctGCTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattg            <  1:4190347/93‑1 (MQ=255)

                                                                            ctctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattg            >  2:2413159/1‑93 (MQ=255)

                                                                            ctctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattg            >  2:2698486/1‑93 (MQ=255)

                                                                            ctctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattg            >  2:3750438/1‑93 (MQ=255)

                                                                            ctctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattg            >  1:3129300/1‑93 (MQ=255)

                                                                             tctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgc           >  1:1174001/1‑93 (MQ=255)

                                                                             tctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgc           >  1:3259699/1‑93 (MQ=255)

                                                                              ctACTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg          >  2:1693340/1‑93 (MQ=255)

                                                                                aCTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcgca        >  2:4400847/1‑93 (MQ=255)

                                                                                aCTCTTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcgca        >  2:1154661/1‑93 (MQ=255)

                                                                                aCTCTTCGAGCGATTTGATGAAATCGCCTTCAATACAGGCATGCTGGATAAATCTGTTATTTTGAAAACCGCGGGTTATttgcgcatggcgca        >  2:733496/1‑93 (MQ=255)

                                                                                 ctctTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCaa       >  1:100997/1‑93 (MQ=255)

                                                                                   ctTCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAAcc     >  1:4158597/1‑93 (MQ=255)

                                                                                    ttCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCa    <  2:4652706/93‑1 (MQ=255)

                                                                                    ttCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCa    >  1:521577/1‑93 (MQ=255)

                                                                                    ttCGAGCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCa    >  1:2656821/1‑93 (MQ=255)

GATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGA  >  NC_000913/1871895‑1872073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`