BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,795	T→C	S60G (AGT→GGT)	yeaR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,795	0	T	C	98.6%	455.2 / ‑2.8	143	S60G (AGT→GGT)	yeaR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (64/77); total (65/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 155 total aligned reads displayed.

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                          <  1:105932/93‑1 (MQ=255)

 agtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgtg                                                                                         >  2:269338/1‑93 (MQ=255)

  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                        <  2:2132137/93‑1 (MQ=255)

   tcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGtt                                                                                       >  1:447919/1‑93 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                   <  2:1236512/93‑1 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                   <  1:7947/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:652177/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:185506/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:2751529/93‑1 (MQ=255)

          ttCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGc                                                                                <  2:1858569/93‑1 (MQ=255)

            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                              <  2:2952525/93‑1 (MQ=255)

             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                             >  1:552541/1‑93 (MQ=255)

             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                             <  1:108367/93‑1 (MQ=255)

              aTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAg                                                                            >  2:1607342/1‑93 (MQ=255)

               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           <  1:4498662/93‑1 (MQ=255)

               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           <  2:1236841/93‑1 (MQ=255)

               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           >  1:13478/1‑93 (MQ=255)

                 ttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                         >  1:2055421/1‑93 (MQ=255)

                 ttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                         <  1:865703/93‑1 (MQ=255)

                   gtgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgag                                                                       >  2:968797/1‑93 (MQ=255)

                   gtgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgag                                                                       <  1:1424826/93‑1 (MQ=255)

                    tgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgaga                                                                      <  1:3249270/93‑1 (MQ=255)

                        ccTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCCCCGTGTTCATCAGCGTAGCCGAGATAtt                                                                  <  1:3575567/92‑1 (MQ=255)

                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                 <  1:770638/93‑1 (MQ=255)

                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                 >  2:2191732/1‑93 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                                <  1:304004/93‑1 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                                >  1:4616644/1‑93 (MQ=255)

                             tCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAcc                                                             <  2:3799688/93‑1 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                           >  2:3603939/1‑93 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                           >  2:4447046/1‑93 (MQ=255)

                                ggAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcc                                                          >  1:2177290/1‑93 (MQ=255)

                                  aGGGAACACCGCAAACTGCCCCGCTTCGATAATGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcccc                                                        <  1:3222618/93‑1 (MQ=255)

                                   gggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCa                                                       <  1:3353699/93‑1 (MQ=255)

                                    ggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCAt                                                      >  2:4631422/1‑93 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  1:1974876/1‑93 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  1:2968795/1‑93 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  2:3276472/1‑93 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGCCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   <  2:3179125/93‑1 (MQ=255)

                                        cacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCAt                                                  <  2:1568247/93‑1 (MQ=255)

                                        cacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCAt                                                  <  1:4520700/93‑1 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                                <  1:149255/93‑1 (MQ=255)

                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                               <  1:2842856/93‑1 (MQ=255)

                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                               <  1:2842855/93‑1 (MQ=255)

                                            gCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACg                                              >  2:2121458/1‑93 (MQ=255)

                                               aaCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaa                                           >  2:616637/1‑93 (MQ=255)

                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGTATAACGGaaa                                          <  1:4194022/93‑1 (MQ=255)

                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaaa                                          <  1:2042245/93‑1 (MQ=255)

                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                         >  1:2415469/1‑93 (MQ=255)

                                                  tGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAgg                                        >  1:1445810/1‑93 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                       >  1:2679030/1‑93 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                       >  1:3640067/1‑93 (MQ=255)

                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                      >  2:2572105/1‑93 (MQ=255)

                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                      >  1:1071967/1‑93 (MQ=255)

                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCACGCATAACGGAAAGGCg                                      >  1:4223406/1‑93 (MQ=255)

                                                     cccGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  1:4377884/93‑1 (MQ=255)

                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  2:3210429/93‑1 (MQ=255)

                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  1:1334821/93‑1 (MQ=255)

                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  2:1447609/93‑1 (MQ=255)

                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    <  2:1049280/93‑1 (MQ=255)

                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    >  1:892842/1‑93 (MQ=255)

                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    >  2:4450576/1‑93 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGtcg                                   >  2:2559014/1‑91 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                   >  2:3738076/1‑93 (MQ=255)

                                                        gCTTGGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  <  1:3988099/93‑1 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3312563/1‑93 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3943328/1‑93 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3739579/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                >  1:2425629/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                <  2:2988487/93‑1 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                <  2:614992/93‑1 (MQ=255)

                                                            cGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGaaaa                              >  1:994160/1‑93 (MQ=255)

                                                             gATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑c                            >  2:3957792/1‑93 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑cc                           >  1:295213/1‑93 (MQ=255)

                                                               tAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCACCATGCATAACGGAAAGGCGTGGGTAAA‑ccc                          >  2:4596371/1‑93 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        <  1:413973/93‑1 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        >  1:1959561/1‑93 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        >  2:4271882/1‑93 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        <  1:4380235/93‑1 (MQ=255)

                                                                  ggATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCg                       >  1:3527132/1‑93 (MQ=255)

                                                                  ggATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCg                       <  1:70332/93‑1 (MQ=255)

                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCgg                      <  2:1636299/93‑1 (MQ=255)

                                                                       aCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGgcgc                  <  2:3878798/93‑1 (MQ=255)

                                                                          tGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGtt               >  1:289854/1‑93 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTcc             >  1:128070/1‑93 (MQ=255)

                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCCGGGCGCGTTcc             <  2:2693358/93‑1 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCtt           <  1:1038184/93‑1 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCtt           >  2:3983532/1‑93 (MQ=255)

                                                                               aGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCttt          <  2:4222681/93‑1 (MQ=255)

                                                                               aGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCttt          >  1:4687849/1‑93 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTa         >  2:343386/1‑93 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTa         <  1:979216/93‑1 (MQ=255)

                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  2:1947928/93‑1 (MQ=255)

                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  1:3848271/93‑1 (MQ=255)

                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  1:519723/93‑1 (MQ=255)

                                                                                   tctGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCa      >  2:3680604/1‑93 (MQ=255)

                                                                                    ctGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCaa     >  1:2572490/1‑93 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGa   <  2:3882372/93‑1 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGa   >  1:2294653/1‑93 (MQ=255)

                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGGGTTTCTTTATCAAGAt  >  2:4241756/1‑93 (MQ=255)

                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAt  <  2:3837838/93‑1 (MQ=255)

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`