BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,485	T→C	E170G (GAA→GGA)	b1838 ←	protein phosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,485	0	T	C	99.3%	437.4 / ‑5.0	138	E170G (GAA→GGA)	b1838	protein phosphatase 1
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (79/58); total (80/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 141 total aligned reads displayed.

TGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA  >  NC_000913/1920395‑1920574

tGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCa                                                                                         <  2:4536459/93‑1 (MQ=255)

 gCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCAc                                                                                        >  2:4089292/1‑93 (MQ=255)

   aGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCt                                                                                      <  1:661598/93‑1 (MQ=255)

   aGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTACCTGCCCTTTTTGGCGTCCACCt                                                                                      >  2:4231744/1‑93 (MQ=255)

      ttGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTCAt                                                                                   >  2:1872209/1‑93 (MQ=255)

       tGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATc                                                                                  >  1:4601305/1‑93 (MQ=255)

         ccAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATcgc                                                                                >  2:400529/1‑93 (MQ=255)

            atatCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAg                                                                             >  1:2821047/1‑93 (MQ=255)

            atatCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAg                                                                             >  2:4047255/1‑93 (MQ=255)

             tatCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGc                                                                            <  2:1208004/93‑1 (MQ=255)

              atCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCg                                                                           >  2:1855962/1‑93 (MQ=255)

               tCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCgg                                                                          >  2:1781741/1‑93 (MQ=255)

               tCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCgg                                                                          >  2:4086746/1‑93 (MQ=255)

               tCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCgg                                                                          >  1:3434681/1‑93 (MQ=255)

                ccACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGc                                                                         <  1:4421332/93‑1 (MQ=255)

                 cACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCt                                                                        >  2:3196681/1‑93 (MQ=255)

                 cACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCt                                                                        >  2:455695/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  2:1665364/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  1:396555/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  1:396554/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  1:866368/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  1:1681440/1‑93 (MQ=255)

                  aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTGTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  1:2668763/1‑93 (MQ=255)

                       aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACaa                                                                  <  2:2128084/93‑1 (MQ=255)

                       aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACATAATCGCGAGCGGCTCCACaa                                                                  >  2:2840531/1‑93 (MQ=255)

                          tCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAc                                                               >  2:2381339/1‑93 (MQ=255)

                            gCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCt                                                             <  2:1066506/93‑1 (MQ=255)

                            gCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCACCACAAGACCt                                                             >  2:1979583/1‑93 (MQ=255)

                             cAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTg                                                            <  1:2753390/93‑1 (MQ=255)

                             cAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGATCCACAAGACCTg                                                            >  2:4688419/1‑93 (MQ=255)

                              aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           <  2:326636/93‑1 (MQ=255)

                               aCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAt                                                          <  1:376569/93‑1 (MQ=255)

                                cGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATg                                                         >  1:4503791/1‑93 (MQ=255)

                                  gtgtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCa                                                       >  2:1368364/1‑93 (MQ=255)

                                   tgtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaa                                                      >  2:2786814/1‑93 (MQ=255)

                                   tgtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGACGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaa                                                      >  1:4703730/1‑93 (MQ=255)

                                    gtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaaa                                                     <  2:2794989/93‑1 (MQ=255)

                                    gtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaaa                                                     >  1:158612/1‑93 (MQ=255)

                                      aTGACCAAACCAGAAATCATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATc                                                   <  2:3227144/93‑1 (MQ=255)

                                       tGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCa                                                  >  1:4430024/1‑93 (MQ=255)

                                       tGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCa                                                  >  1:1323377/1‑93 (MQ=255)

                                         aCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAAc                                                <  1:312216/93‑1 (MQ=255)

                                         aCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAAc                                                >  2:1252998/1‑93 (MQ=255)

                                         aCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAAc                                                <  1:2885879/93‑1 (MQ=255)

                                          ccAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACg                                               >  1:1106557/1‑93 (MQ=255)

                                            aaaCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTc                                             <  1:1403262/93‑1 (MQ=255)

                                            aaaCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTc                                             <  2:2552690/93‑1 (MQ=255)

                                              aCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCt                                           <  2:2330121/93‑1 (MQ=255)

                                              aCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCt                                           >  2:1230246/1‑93 (MQ=255)

                                              aCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCt                                           >  1:1890476/1‑93 (MQ=255)

                                               ccAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCtt                                          >  1:3507090/1‑93 (MQ=255)

                                                cAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCttt                                         <  1:1774830/93‑1 (MQ=255)

                                                  gAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTg                                       <  1:2683549/93‑1 (MQ=255)

                                                    aaTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGcc                                     >  2:2310/1‑93 (MQ=255)

                                                     aTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCa                                    >  2:4737299/1‑93 (MQ=255)

                                                      tGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAt                                   <  1:869104/93‑1 (MQ=255)

                                                      tGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAt                                   <  1:2606765/93‑1 (MQ=255)

                                                       gATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAtt                                  <  1:755370/93‑1 (MQ=255)

                                                         tCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCa                                >  1:3556259/1‑93 (MQ=255)

                                                          cAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAt                               <  2:4568755/93‑1 (MQ=255)

                                                          cAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAt                               >  2:3329671/1‑93 (MQ=255)

                                                          cAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAt                               >  2:719779/1‑93 (MQ=255)

                                                           aGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATa                              <  1:2809547/93‑1 (MQ=255)

                                                           aGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCATTTTGCCATTCATa                              >  2:3587824/1‑93 (MQ=255)

                                                            gCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGGGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaa                             >  2:2708860/1‑93 (MQ=255)

                                                            gCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaa                             <  1:3384315/93‑1 (MQ=255)

                                                            gCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaa                             <  1:4509732/93‑1 (MQ=255)

                                                             cACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaaa                            <  2:2177090/93‑1 (MQ=255)

                                                             cACCTATAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaaa                            >  2:174677/1‑93 (MQ=255)

                                                              aCCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAc                           <  2:4343315/93‑1 (MQ=255)

                                                              aCCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAc                           <  2:386410/93‑1 (MQ=255)

                                                               ccTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGTCATTCATAAAca                          >  2:4208147/1‑93 (MQ=255)

                                                               ccTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAca                          <  1:451322/93‑1 (MQ=255)

                                                                cTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcat                         >  1:4024285/1‑93 (MQ=255)

                                                                 tGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatc                        >  1:1546087/1‑93 (MQ=255)

                                                                  gTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatca                       >  2:2848983/1‑93 (MQ=255)

                                                                  gTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatca                       <  1:2070773/93‑1 (MQ=255)

                                                                   tAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatcat                      <  1:2360518/93‑1 (MQ=255)

                                                                   tAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatcat                      <  1:4356809/93‑1 (MQ=255)

                                                                    aaTTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACatcatc                     >  2:1161674/1‑93 (MQ=255)

                                                                     aTTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCt                    >  1:209474/1‑93 (MQ=255)

                                                                      ttCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTg                   >  1:4116369/1‑93 (MQ=255)

                                                                      ttCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTg                   <  2:2034168/93‑1 (MQ=255)

                                                                       tCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTgg                  <  2:4569075/93‑1 (MQ=255)

                                                                        cccTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGa                 >  1:3142774/1‑93 (MQ=255)

                                                                        cccTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGa                 <  2:266352/93‑1 (MQ=255)

                                                                        cccTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGa                 <  1:375676/93‑1 (MQ=255)

                                                                            gCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAt             >  1:1268690/1‑93 (MQ=255)

                                                                            gCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAt             >  2:2846230/1‑93 (MQ=255)

                                                                            gCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAt             >  2:2724355/1‑93 (MQ=255)

                                                                               cTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCgg          >  2:3498653/1‑93 (MQ=255)

                                                                               cTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCgg          <  2:3015646/93‑1 (MQ=255)

                                                                                tttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGc         >  2:1907650/1‑93 (MQ=255)

                                                                                 ttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCa        <  1:2451026/93‑1 (MQ=255)

                                                                                 ttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCa        <  1:1390534/93‑1 (MQ=255)

                                                                                 ttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCa        <  2:827316/93‑1 (MQ=255)

                                                                                 ttgtGGCGTCCACGTAATTGCGAGCGGCTCCACAAGACCTTATGCACAGCAGAGTCCTTTTGCCAGTCATAAACATCATCTGGATAATCGGCa        <  1:731937/90‑1 (MQ=255)

                                                                                   ttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATg      <  2:1735993/93‑1 (MQ=255)

                                                                                    tGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGa     <  1:2199032/93‑1 (MQ=255)

                                                                                       cGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCa  <  2:4467453/93‑1 (MQ=255)

TGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA  >  NC_000913/1920395‑1920574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`