BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,564	T→C	T70A (ACC→GCC)	yebG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,564	0	T	C	98.1%	501.6 / ‑5.5	159	T70A (ACC→GCC)	yebG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (71/85); total (73/86)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

AAGTTGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGTGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGCCAGG  >  NC_000913/1928473‑1928653

aaGTTGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGCCGGTATTTTGCCGGTTTTCAGAATGGTg                                                                                          >  1:4039576/1‑93 (MQ=255)

 aGTTGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGgcgc                                                                                         >  1:86037/1‑93 (MQ=255)

    tGCTTCAGGGCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCa                                                                                      <  1:4716757/93‑1 (MQ=255)

    tGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCa                                                                                      >  2:3144729/1‑93 (MQ=255)

    tGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCa                                                                                      <  1:306836/93‑1 (MQ=255)

    tGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCa                                                                                      <  1:2860223/93‑1 (MQ=255)

    tGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCa                                                                                      <  2:801799/93‑1 (MQ=255)

     gCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAg                                                                                     >  1:1425776/1‑93 (MQ=255)

     gCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAg                                                                                     <  2:471992/93‑1 (MQ=255)

       ttCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGca                                                                                   >  2:2746945/1‑93 (MQ=255)

         cAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGcaca                                                                                 <  2:2701621/93‑1 (MQ=255)

         cAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGcaca                                                                                 >  1:2267209/1‑93 (MQ=255)

          aGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACAt                                                                                <  1:3450654/93‑1 (MQ=255)

             ccGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCtt                                                                             <  2:228937/93‑1 (MQ=255)

              cgcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCttt                                                                            <  1:1991241/93‑1 (MQ=255)

              cgcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCttt                                                                            <  2:602061/93‑1 (MQ=255)

               gcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCtttt                                                                           <  2:1054294/93‑1 (MQ=255)

               gcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCtttt                                                                           <  1:3616823/93‑1 (MQ=255)

               gcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCtttt                                                                           <  2:474070/93‑1 (MQ=255)

               gcgTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCtttt                                                                           >  2:2467736/1‑93 (MQ=255)

                  tgtgAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGt                                                                        >  2:2891860/1‑93 (MQ=255)

                  tgtgAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGt                                                                        >  2:3338578/1‑93 (MQ=255)

                   gtgAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGtt                                                                       <  2:1557985/93‑1 (MQ=255)

                    tgAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTc                                                                      <  2:1066901/93‑1 (MQ=255)

                     gAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCt                                                                     <  1:1763957/93‑1 (MQ=255)

                         cATCTTCCTCTTCGGATTCAGCACCGACGCCCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgcca                                                                 <  2:4371632/93‑1 (MQ=255)

                         cATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgcca                                                                 <  1:2520283/93‑1 (MQ=255)

                          aTCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccag                                                                >  2:4258657/1‑93 (MQ=255)

                          aTCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccag                                                                >  1:1495938/1‑93 (MQ=255)

                            cTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccagcc                                                              >  1:2724028/1‑93 (MQ=255)

                            cTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccagcc                                                              <  1:1435651/93‑1 (MQ=255)

                            cTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccagcc                                                              <  1:4406108/93‑1 (MQ=255)

                             ttCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccagcca                                                             <  2:2014284/93‑1 (MQ=255)

                             ttCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTgccagcca                                                             <  2:4233738/93‑1 (MQ=255)

                              tCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCAt                                                            <  2:3170488/93‑1 (MQ=255)

                               ccTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATa                                                           <  2:2084919/93‑1 (MQ=255)

                               ccTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATa                                                           <  1:3263626/93‑1 (MQ=255)

                                ctctTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAg                                                          <  1:156275/93‑1 (MQ=255)

                                ctctTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAg                                                          <  1:1392225/93‑1 (MQ=255)

                                ctctTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTCTTGTTCTGCCAGCCATAg                                                          <  2:4657361/93‑1 (MQ=255)

                                 tctTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGc                                                         >  2:3700027/1‑93 (MQ=255)

                                  ctTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCg                                                        >  2:2720253/1‑93 (MQ=255)

                                   ttCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGa                                                       >  1:3963056/1‑93 (MQ=255)

                                   ttCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGa                                                       >  1:2370246/1‑93 (MQ=255)

                                    tCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGaa                                                      >  2:1900635/1‑93 (MQ=255)

                                     cGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGaaa                                                     <  1:3313895/93‑1 (MQ=255)

                                      ggATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAg                                                    <  2:3461758/93‑1 (MQ=255)

                                       gATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAgg                                                   >  1:2353177/1‑93 (MQ=255)

                                        aTTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAggg                                                  <  1:959253/93‑1 (MQ=255)

                                         ttCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGc                                                 >  2:2636061/1‑93 (MQ=255)

                                          tCAGCACCCACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCt                                                <  1:1319292/93‑1 (MQ=255)

                                            aGCACCGACTACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCTCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTc                                              <  2:4256264/93‑1 (MQ=255)

                                            aGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTc                                              <  2:3946688/93‑1 (MQ=255)

                                            aGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTc                                              <  1:104982/93‑1 (MQ=255)

                                             gCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCa                                             >  1:1594230/1‑93 (MQ=255)

                                              cACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCAc                                            >  1:3812892/1‑93 (MQ=255)

                                              cACCGACGACCGGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCAc                                            <  2:410333/93‑1 (MQ=255)

                                                ccGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGt                                          >  1:1316314/1‑93 (MQ=255)

                                                 cgacgaCCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGtt                                         >  1:470013/1‑93 (MQ=255)

                                                 cgacgaCCTGTGGGGACGGTAATGTGCCGGTTTTCAGAATGGCGCTCAGCACTTCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTGCACGtt                                         <  2:1686564/93‑1 (MQ=255)

                                                  gacgacCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTg                                        >  2:1782931/1‑93 (MQ=255)

                                                  gacgacCTGTGGGGACGGTAATTTGCCGGGTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTg                                        >  2:2449041/1‑93 (MQ=255)

                                                   acgacCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGc                                       >  2:827457/1‑93 (MQ=255)

                                                   acgacCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGc                                       <  2:1808948/93‑1 (MQ=255)

                                                      acCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCg                                    >  2:1573220/1‑93 (MQ=255)

                                                       ccTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGt                                   >  1:4316461/1‑93 (MQ=255)

                                                        cTGTGGGGACGCTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTTGGCGAGCCAATGCGAAAGGGCTTCACGTTGCTCGTc                                  <  2:258254/93‑1 (MQ=255)

                                                         tgtgGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCt                                 >  1:4236822/1‑93 (MQ=255)

                                                         tgtgGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCt                                 <  1:4605752/93‑1 (MQ=255)

                                                          gtgGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCtt                                >  1:360264/1‑93 (MQ=255)

                                                          gtgGGGACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCtt                                <  2:4396844/93‑1 (MQ=255)

                                                            ggggACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTcc                              >  2:4520018/1‑93 (MQ=255)

                                                            ggggACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTcc                              <  1:1890370/93‑1 (MQ=255)

                                                             gggACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCa                             <  1:1184709/93‑1 (MQ=255)

                                                             gggACGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCa                             >  1:4401321/1‑93 (MQ=255)

                                                                 cGGTAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTg                         >  1:3926100/1‑93 (MQ=255)

                                                                    tAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCAc                      <  2:3333740/93‑1 (MQ=255)

                                                                    tAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCAc                      >  2:1715790/1‑93 (MQ=255)

                                                                    tAATTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCAc                      >  2:1660678/1‑93 (MQ=255)

                                                                     aaTTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACt                     <  2:3021146/93‑1 (MQ=255)

                                                                      aTTTGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTg                    >  1:426635/1‑93 (MQ=255)

                                                                        ttGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGga                  <  2:3126621/93‑1 (MQ=255)

                                                                        ttGCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGga                  >  2:4047146/1‑93 (MQ=255)

                                                                          gCCGGTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGgaga                >  2:3536351/1‑93 (MQ=255)

                                                                             ggTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAAtt             <  2:2927248/93‑1 (MQ=255)

                                                                             ggTTTCCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAAtt             >  1:2611985/1‑93 (MQ=255)

                                                                              gTTTTCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAAttt            <  1:2186135/93‑1 (MQ=255)

                                                                                 ttCAGAATGGCGCTCAGCACATCATTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTc         <  2:3376579/93‑1 (MQ=255)

                                                                                  tCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCa        >  2:2538082/1‑93 (MQ=255)

                                                                                  tCAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCa        >  2:747869/1‑93 (MQ=255)

                                                                                  tCACAATGGCGCTCAGCACATCTTTTTGTTCTGCGAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCa        <  1:797312/93‑1 (MQ=255)

                                                                                   cAGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAg       >  2:4429785/1‑93 (MQ=255)

                                                                                    aGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGc      <  2:4301755/93‑1 (MQ=255)

                                                                                    aGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGc      >  2:2171707/1‑93 (MQ=255)

                                                                                    aGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGc      >  2:4605628/1‑93 (MQ=255)

                                                                                    aGAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGc      >  2:3709198/1‑93 (MQ=255)

                                                                                     gAATGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGcc     <  1:3511143/93‑1 (MQ=255)

                                                                                       aTGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGCCAg   <  1:3037771/93‑1 (MQ=255)

                                                                                       aTGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGCCAg   >  1:2529078/1‑93 (MQ=255)

                                                                                        tGGCGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGCCAgg  >  2:1201116/1‑93 (MQ=255)

AAGTTGCTTCAGGCCGCGTGTGAGGCATCTTCCTCTTCGGATTCAGCACCGACGACCTGTGGGGACGGTAATTTGCCGGTTTTCAGAATGGTGCTCAGCACATCTTTTTGTTCTGCCAGCCATAGCGAAAGGGCTTCACGTTGCTCGTCTTCCATTTGCACTGGAGAATTTGTCAGCCAGG  >  NC_000913/1928473‑1928653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`