BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,713	T→C	S92S (AGT→AGC)	yebI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,713	0	T	C	97.0%	394.8 / 1.7	133	S92S (AGT→AGC)	yebI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (58/69); total (62/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 136 total aligned reads displayed.

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

gTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGcc                                                                                        <  1:1241233/93‑1 (MQ=255)

gTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGcc                                                                                        <  2:1192133/93‑1 (MQ=255)

 ttACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGccc                                                                                       <  2:2342237/93‑1 (MQ=255)

  tACGCTGCTGCTGGCGTGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACCCGTGATTAGGGGTTATGGCGCACAGCGCCCt                                                                                      >  2:80202/1‑93 (MQ=255)

  tACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCt                                                                                      <  1:406064/93‑1 (MQ=255)

     gctgctgctGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTc                                                                                   <  2:4275730/93‑1 (MQ=255)

      ctgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTcc                                                                                  >  1:3301956/1‑92 (MQ=255)

      ctgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCg                                                                                  <  1:1061088/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                <  1:1752131/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                >  1:46412/1‑93 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              <  1:2703433/93‑1 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              <  1:231560/93‑1 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCt                                                                          >  2:2502236/1‑93 (MQ=255)

                 gtgcgcTCTGTTAGGGCTGGAGAAGCTTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtggt                                                                       <  2:4123139/91‑1 (MQ=255)

                 gggcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtggt                                                                       <  1:2318316/93‑1 (MQ=255)

                 gggcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGACCTGTCGCTGGGCCtggt                                                                       <  1:978182/93‑1 (MQ=255)

                      gTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGAAACGTTATTAGGGATTATGGCGCACAGAGCCCTGTCGCTGGGCCTGGTGGTCg                                                                  >  1:673609/1‑93 (MQ=255)

                        cTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTg                                                                <  1:900600/93‑1 (MQ=255)

                        cTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTg                                                                >  1:3904205/1‑93 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTgg                                                               >  1:2574302/1‑92 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              >  1:1026465/1‑93 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              <  1:1457195/93‑1 (MQ=255)

                            tATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            >  1:3902993/1‑93 (MQ=255)

                            tATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            >  1:3902992/1‑93 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGAGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCt                                                           >  1:734456/1‑93 (MQ=255)

                              tGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTg                                                          >  2:2009363/1‑93 (MQ=255)

                                 cTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATg                                                       <  1:4488701/93‑1 (MQ=255)

                                  tGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGt                                                      <  2:1754050/93‑1 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTc                                                     <  1:417586/93‑1 (MQ=255)

                                    gagaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCt                                                    <  2:2109695/93‑1 (MQ=255)

                                      gaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTaa                                                  >  2:3131902/1‑93 (MQ=255)

                                       aaGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAat                                                 >  2:1381674/1‑93 (MQ=255)

                                       aaGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAat                                                 >  1:1446667/1‑93 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                <  1:825211/93‑1 (MQ=255)

                                           gTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTc                                             <  1:3040950/93‑1 (MQ=255)

                                           gTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGGGGTCGTGAGTCTGATGTCTAATATTc                                             >  1:2238672/1‑93 (MQ=255)

                                            tCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCg                                            >  1:276196/1‑93 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGTATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           <  2:1697820/93‑1 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           >  1:2941877/1‑93 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           <  1:2972548/93‑1 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           >  1:280544/1‑93 (MQ=255)

                                              cacaGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgtg                                          <  1:2464510/93‑1 (MQ=255)

                                              cacaGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgtg                                          >  2:3840262/1‑93 (MQ=255)

                                               acaGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgtgt                                         >  2:4015634/1‑93 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGTGTCTGATGTCTAATATTCGTGtt                                        >  1:770709/1‑93 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        <  2:4467747/93‑1 (MQ=255)

                                                 aGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttg                                       >  2:4566144/1‑93 (MQ=255)

                                                  ggtGGCGGTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      <  1:3585549/91‑1 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      <  2:3452106/93‑1 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      <  1:3585548/93‑1 (MQ=255)

                                                   cTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgat                                     <  1:1714135/93‑1 (MQ=255)

                                                   cTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgat                                     <  1:155359/93‑1 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    >  2:4570611/1‑93 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    <  1:1510180/93‑1 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    <  1:2486974/93‑1 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    >  2:3740539/1‑93 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    <  2:2892944/93‑1 (MQ=255)

                                                     ggCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgattt                                   >  1:1849478/1‑93 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGACCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 <  1:2550495/93‑1 (MQ=255)

                                                        gATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttgat                                >  1:2953641/1‑93 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               <  1:2587777/93‑1 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               <  1:3779976/93‑1 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               >  2:550865/1‑93 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               >  1:4132645/1‑93 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATACTCGTGTTGATTTGATg                               >  1:2341256/1‑93 (MQ=255)

                                                          tCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATgg                              >  1:3391411/1‑93 (MQ=255)

                                                           cGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGc                             <  1:3427444/93‑1 (MQ=255)

                                                           cGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGc                             <  1:66068/93‑1 (MQ=255)

                                                           cGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGc                             <  1:1447833/93‑1 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  2:1393605/1‑93 (MQ=255)

                                                             acacGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCtt                           >  2:740537/1‑93 (MQ=255)

                                                              cacGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTa                          <  1:3206477/93‑1 (MQ=255)

                                                              cacGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTa                          >  1:811404/1‑93 (MQ=255)

                                                              cacGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTa                          >  2:3240344/1‑93 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  1:4170683/1‑93 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  1:4680095/1‑93 (MQ=255)

                                                                 gTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCt                       >  2:3552648/1‑93 (MQ=255)

                                                                   tattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGt                     >  2:138214/1‑93 (MQ=255)

                                                                    attaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGtt                    <  1:2222522/93‑1 (MQ=255)

                                                                     ttaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTc                   <  1:4018489/93‑1 (MQ=255)

                                                                     ttaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTc                   <  2:2292505/93‑1 (MQ=255)

                                                                      taGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCg                  >  1:697985/1‑93 (MQ=255)

                                                                      taGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCg                  <  2:1122921/93‑1 (MQ=255)

                                                                       aGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCgg                 <  2:514426/93‑1 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                <  2:569507/93‑1 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                >  2:398955/1‑93 (MQ=255)

                                                                          gATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGa              <  1:927573/93‑1 (MQ=255)

                                                                           aTTATGGCGCGCAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAt             <  2:149726/93‑1 (MQ=255)

                                                                           aTTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAt             <  1:3300330/93‑1 (MQ=255)

                                                                             tATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAttt           <  2:205570/93‑1 (MQ=255)

                                                                             tATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAttt           >  2:3188577/1‑93 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          >  1:542587/1‑93 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          <  1:2444370/93‑1 (MQ=255)

                                                                               tGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGc         <  1:1530166/93‑1 (MQ=255)

                                                                                ggCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCt        <  1:367175/93‑1 (MQ=255)

                                                                                  cgcACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTgg      >  2:2703973/1‑93 (MQ=255)

                                                                                   gcACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGc     >  2:3286459/1‑93 (MQ=255)

                                                                                    cacaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCa    >  2:3624484/1‑93 (MQ=255)

                                                                                    cacaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCa    <  1:806381/93‑1 (MQ=255)

                                                                                      caGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGt  >  2:276227/1‑93 (MQ=255)

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`