BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,958,588	T→C	M168T (ATG→ACG)	argS →	arginine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,958,588	0	T	C	98.6%	455.9 / ‑2.6	145	M168T (ATG→ACG)	argS	arginine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (59/84); total (60/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

Only 100 of 148 total aligned reads displayed.

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAA  >  NC_000913/1958498‑1958676

 tGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCt                                                                                       <  2:4353580/93‑1 (MQ=255)

  ggTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCAGTACGCTg                                                                                      <  1:788350/93‑1 (MQ=255)

   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                     >  1:3284053/1‑93 (MQ=255)

   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                     >  2:365558/1‑93 (MQ=255)

    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                    <  2:2112038/93‑1 (MQ=255)

    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                    <  2:2868512/93‑1 (MQ=255)

     gACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTAAGTTCGGTACGCTGAtt                                                                                   <  1:1326121/93‑1 (MQ=255)

        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                                <  1:3999838/93‑1 (MQ=255)

         cagcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAt                                                                               >  1:2934246/1‑93 (MQ=255)

           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                             >  1:3699065/1‑93 (MQ=255)

           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                             >  1:2118845/1‑93 (MQ=255)

            cagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGc                                                                            <  2:2157005/93‑1 (MQ=255)

            cagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGc                                                                            <  1:1756269/93‑1 (MQ=255)

              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          <  2:4278688/93‑1 (MQ=255)

              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          >  2:2280570/1‑93 (MQ=255)

              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          >  2:580986/1‑93 (MQ=255)

               tGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTgg                                                                         >  2:934267/1‑93 (MQ=255)

               tGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTgg                                                                         >  1:3966973/1‑93 (MQ=255)

                 cGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaa                                                                       <  1:669614/93‑1 (MQ=255)

                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                     >  1:2709446/1‑93 (MQ=255)

                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                     <  1:616775/93‑1 (MQ=255)

                    aCTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAag                                                                    >  2:2905893/1‑93 (MQ=255)

                     ctctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagc                                                                   <  2:3498224/93‑1 (MQ=255)

                     ctctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagc                                                                   >  1:3395616/1‑93 (MQ=255)

                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                  >  1:4603497/1‑93 (MQ=255)

                       ctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcag                                                                 <  1:462692/93‑1 (MQ=255)

                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  1:3179824/93‑1 (MQ=255)

                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  2:4639655/93‑1 (MQ=255)

                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  1:4352524/93‑1 (MQ=255)

                         ggAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagca                                                               <  1:1231012/93‑1 (MQ=255)

                          gAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagcag                                                              <  2:599891/93‑1 (MQ=255)

                            ggtCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                            >  2:138985/3‑93 (MQ=255)

                             ttCCTGGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAGGGCTGGAAAAGcagcagcag                                                           <  1:984807/93‑1 (MQ=255)

                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                        >  1:973670/1‑93 (MQ=255)

                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                        >  1:223186/1‑93 (MQ=255)

                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                       >  2:1395408/1‑93 (MQ=255)

                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                       <  2:3780792/93‑1 (MQ=255)

                                  tggTCACAAAGTGATCCGAACAAAGCGCGTCGGCGACTGCGGCACTCCGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaaa                                                      <  2:2003169/92‑1 (MQ=255)

                                  cGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaaa                                                      <  1:476319/93‑1 (MQ=255)

                                    gTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACg                                                    <  2:3104945/93‑1 (MQ=255)

                                     tCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGc                                                   <  2:1540432/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:1998155/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:1733744/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGGCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:3306791/93‑1 (MQ=255)

                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCggg                                               >  2:25071/1‑92 (MQ=255)

                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCggg                                               >  2:3338709/1‑92 (MQ=255)

                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGt                                               <  2:2419019/93‑1 (MQ=255)

                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                              <  1:2523506/93‑1 (MQ=255)

                                              gATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                          >  1:3115895/1‑93 (MQ=255)

                                              gATTCGCGCAAACCACCTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                          <  1:3802210/93‑1 (MQ=255)

                                               aTTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                         <  1:1802623/93‑1 (MQ=255)

                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                        <  1:2958171/93‑1 (MQ=255)

                                                  cgcgCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAg                                      <  1:1863879/93‑1 (MQ=255)

                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                    >  2:3290190/1‑93 (MQ=255)

                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                    <  1:3187855/93‑1 (MQ=255)

                                                     gcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                   <  1:1020978/93‑1 (MQ=255)

                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                 >  1:316136/1‑93 (MQ=255)

                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                 >  1:4017231/1‑93 (MQ=255)

                                                        aaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggct                                >  2:4716834/1‑93 (MQ=255)

                                                          ccACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGa                              <  2:4095934/93‑1 (MQ=255)

                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             >  1:1567101/1‑93 (MQ=255)

                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             >  2:3705143/1‑93 (MQ=255)

                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             <  2:3025013/93‑1 (MQ=255)

                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                            >  2:2048630/1‑93 (MQ=255)

                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                            <  2:3873655/93‑1 (MQ=255)

                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                           <  2:3451405/93‑1 (MQ=255)

                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                         <  2:3579464/93‑1 (MQ=255)

                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                         >  1:1441400/1‑93 (MQ=255)

                                                                cGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGa                        <  1:3725921/93‑1 (MQ=255)

                                                                 ggCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGaa                       <  1:3577670/93‑1 (MQ=255)

                                                                  gCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAg                      <  1:4540251/93‑1 (MQ=255)

                                                                  gCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAg                      >  2:1305663/1‑93 (MQ=255)

                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                    <  1:2114182/93‑1 (MQ=255)

                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                    >  1:4687402/1‑93 (MQ=255)

                                                                     aCTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGtt                   <  1:121716/93‑1 (MQ=255)

                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                 <  2:1546788/93‑1 (MQ=255)

                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                 <  2:1092610/93‑1 (MQ=255)

                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGCAAAGCAGCAGCAGGAAAACGCCGGGGAAATGGAGCTGGCTGACATTGAAGGTTACt                >  2:2475931/1‑93 (MQ=255)

                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt                <  2:436452/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  2:3133575/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:4057931/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:4373519/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:3247654/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:993888/93‑1 (MQ=255)

                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  2:3788269/93‑1 (MQ=255)

                                                                            cACTCAGTGCGGTACGCTGATTGCATGTCTGGAAAAGCAGCAGCAGGAAACCGCCGGTGAAATGAAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:3247653/93‑1 (MQ=255)

                                                                              cTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgcg          >  1:2397522/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAGCTTGAAGGTTTCTACCGCGa         >  2:2541635/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa         >  1:757773/1‑93 (MQ=255)

                                                                                cAGTTCGGTGCGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  2:1744716/93‑1 (MQ=255)

                                                                                cAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  1:2759733/93‑1 (MQ=255)

                                                                                cAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  1:3814516/93‑1 (MQ=255)

                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg       >  1:1756791/1‑93 (MQ=255)

                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg       >  1:4352111/1‑93 (MQ=255)

                                                                                  gTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGc      >  2:2205235/1‑93 (MQ=255)

                                                                                  gTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGc      >  2:765494/1‑93 (MQ=255)

                                                                                    tCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGa    >  2:2064281/1‑93 (MQ=255)

                                                                                    tCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGa    >  1:1930319/1‑93 (MQ=255)

                                                                                      ggTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTTGCTGACCTTGAAGGTTTCTACCGCGATGCGaaa  >  1:2595526/1‑93 (MQ=255)

                                                                                      ggTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGaaa  >  2:4192578/1‑93 (MQ=255)

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAA  >  NC_000913/1958498‑1958676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`