BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,972,288	G→A	T354M (ACG→ATG)	cheA ←	CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,972,288	0	G	A	98.5%	468.9 / ‑3.8	135	T354M (ACG→ATG)	cheA	CheA
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (67/66); total (68/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

Only 100 of 141 total aligned reads displayed.

ATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGC  >  NC_000913/1972200‑1972375

aTCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAg                                                                                     <  2:3004098/93‑1 (MQ=255)

aTCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAg                                                                                     <  2:279570/93‑1 (MQ=255)

  cGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGtt                                                                                   >  2:3091792/1‑93 (MQ=255)

   gAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                  <  2:4285735/93‑1 (MQ=255)

   gAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                  <  1:4419519/93‑1 (MQ=255)

     ggCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTa                                                                                >  2:479805/1‑93 (MQ=255)

      gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                               >  1:373024/1‑93 (MQ=255)

      gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                               <  2:530754/93‑1 (MQ=255)

        tATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCt                                                                             <  1:221276/93‑1 (MQ=255)

            gCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCtt                                                                         <  2:3625575/93‑1 (MQ=255)

            gCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCtt                                                                         >  2:2798321/1‑93 (MQ=255)

             cGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTg                                                                        >  2:3465716/1‑93 (MQ=255)

              gTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGc                                                                       >  2:2638144/1‑93 (MQ=255)

              gTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGc                                                                       >  2:1357798/1‑93 (MQ=255)

               tcccAGGTTGTTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCCCCAGCATCAGTTCTACCTGCTTGcc                                                                      <  1:23269/91‑1 (MQ=255)

               tACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGcc                                                                      >  1:2189024/1‑93 (MQ=255)

               tACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGcc                                                                      <  2:1340296/93‑1 (MQ=255)

                  cAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGGTCTATCTGCTTGCCGAg                                                                   >  2:3592100/1‑93 (MQ=255)

                    ggTGGGTCAGCGGGTCGGTCATTCGTTCTATCAGGGTTTTGTCAAGTTCACTAGTACTGCCCACCAGCATCAGTTCTACCGGCTTGGCGAGtt                                                                 >  2:3605671/1‑93 (MQ=255)

                     gTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGttt                                                                >  2:395800/1‑93 (MQ=255)

                          tCAGCGGGTCGATAATGCGTTCTATCAGACTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCg                                                           <  2:3026753/93‑1 (MQ=255)

                             gCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCa                                                        <  2:1249258/93‑1 (MQ=255)

                             gCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCa                                                        <  1:3970475/93‑1 (MQ=255)

                             gCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCa                                                        >  2:2010051/1‑93 (MQ=255)

                               gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                      >  1:1658262/1‑93 (MQ=255)

                                 gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                    <  2:2149082/93‑1 (MQ=255)

                                 gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                    <  1:491381/93‑1 (MQ=255)

                                 gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                    >  2:25651/1‑93 (MQ=255)

                                 gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                    >  1:3622716/1‑93 (MQ=255)

                                  tCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCa                                                   <  1:1243219/93‑1 (MQ=255)

                                    gATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACg                                                 >  1:2034370/1‑93 (MQ=255)

                                    gATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCACCAGATCACg                                                 >  1:1677289/1‑93 (MQ=255)

                                     aTAATGCGTTCTATCAGGCTTTTTTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGc                                                >  2:3782989/1‑93 (MQ=255)

                                     aTAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGc                                                >  1:204853/1‑93 (MQ=255)

                                     aTAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGc                                                <  1:160152/93‑1 (MQ=255)

                                      tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                               >  1:1947806/1‑93 (MQ=255)

                                      tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                               >  2:504886/1‑93 (MQ=255)

                                      tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                               >  2:2371985/1‑93 (MQ=255)

                                       aaTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAc                                              >  1:2255369/1‑93 (MQ=255)

                                       aaTGCGTTCTATCAAGCTTTTGTCGAGTTCAGCAGTACTGCTCACCAGCATCATGTTTACCTGGTTGCCGGCGCTTTCCGCCAGAACACGCAc                                              >  2:3954186/1‑93 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             <  2:3184615/93‑1 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             <  1:3975606/93‑1 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             >  2:4401183/1‑93 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             >  1:1220956/1‑93 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             >  1:1941068/1‑93 (MQ=255)

                                        aTGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAcc                                             <  1:4307148/93‑1 (MQ=255)

                                         tGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCa                                            >  1:4094950/1‑93 (MQ=255)

                                          gCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAg                                           <  1:3731132/93‑1 (MQ=255)

                                           cGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGc                                          >  1:2993724/1‑93 (MQ=255)

                                            gTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGcc                                         >  2:2194497/1‑93 (MQ=255)

                                             ttCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCg                                        <  1:4582733/93‑1 (MQ=255)

                                              tCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCgg                                       <  2:1191922/93‑1 (MQ=255)

                                                tATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTTCCGCCAGATGACGCACCAGCCggga                                     >  1:257130/1‑92 (MQ=255)

                                                tATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCgggg                                     <  1:3392913/93‑1 (MQ=255)

                                                 aTCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                    >  2:386776/1‑93 (MQ=255)

                                                  tCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGAt                                   >  2:238809/1‑93 (MQ=255)

                                                   cAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATa                                  >  1:1332089/1‑93 (MQ=255)

                                                   cAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATa                                  <  1:3106304/93‑1 (MQ=255)

                                                   cAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGACTTTTCCCGCCAGATCACGCACCAGCCGGGGATa                                  <  1:3161804/93‑1 (MQ=255)

                                                    aGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAg                                 <  2:4120479/93‑1 (MQ=255)

                                                     ggCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGc                                >  1:651454/1‑93 (MQ=255)

                                                     ggCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGc                                <  1:2631871/93‑1 (MQ=255)

                                                     ggCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGc                                <  2:2189688/93‑1 (MQ=255)

                                                        ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACTAGCCGGGGATAGCGAc                             >  1:1322829/1‑93 (MQ=255)

                                                        ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                             <  1:2312107/93‑1 (MQ=255)

                                                        ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                             <  1:3113002/93‑1 (MQ=255)

                                                         tgtGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACt                            <  1:2906022/91‑1 (MQ=255)

                                                           tGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTaa                          <  1:4715679/93‑1 (MQ=255)

                                                               gAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAAc                      >  1:1472698/1‑93 (MQ=255)

                                                               gAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAAc                      >  2:2547139/1‑93 (MQ=255)

                                                               gAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAAc                      >  1:2837983/1‑93 (MQ=255)

                                                                aGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACa                     <  2:957441/93‑1 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    <  1:1120881/93‑1 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    >  2:1008212/1‑93 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    >  1:1543420/1‑93 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    <  2:4418357/93‑1 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    >  2:4341332/1‑93 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    <  2:4303082/93‑1 (MQ=255)

                                                                 gTTCAGTAGAACTGCCCACCAACATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                    >  1:4159834/1‑93 (MQ=255)

                                                                   tCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACatat                  <  1:4635420/93‑1 (MQ=255)

                                                                   tCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACatat                  <  2:3997067/93‑1 (MQ=255)

                                                                    cAGTAGAGCTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATAtt                 <  2:131548/93‑1 (MQ=255)

                                                                    cAGTAGAACTGCCCCCCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATAtt                 <  1:4509783/93‑1 (MQ=255)

                                                                        aGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCAt             >  2:4375852/1‑93 (MQ=255)

                                                                        aGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCAt             <  1:745768/93‑1 (MQ=255)

                                                                        aGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGG‑TAGCGACTAAAAACATATTCCATc            >  2:1483596/1‑93 (MQ=255)

                                                                         gAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATc            <  2:716609/93‑1 (MQ=255)

                                                                          aaCTGCCCACCAGCATCAGTTCTCCCTGCTTGCCGAGTTTTCCCGCAAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCg           <  1:1868347/93‑1 (MQ=255)

                                                                           aCTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCgg          >  2:4235315/1‑93 (MQ=255)

                                                                           aCTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCgg          <  2:942144/93‑1 (MQ=255)

                                                                            cTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCgtc         >  1:4586801/1‑91 (MQ=255)

                                                                               cccACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc      <  1:2844319/93‑1 (MQ=255)

                                                                                  cccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg   <  1:3368934/92‑1 (MQ=255)

                                                                                  accaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg   >  2:773985/1‑93 (MQ=255)

                                                                                  accaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg   >  1:4386995/1‑93 (MQ=255)

                                                                                   ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc  >  1:587274/1‑93 (MQ=255)

                                                                                   ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc  <  2:4525125/93‑1 (MQ=255)

                                                                                   ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc  >  1:296378/1‑93 (MQ=255)

                                                                                   ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc  >  1:587273/1‑93 (MQ=255)

                                                                                   ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc  <  1:4392865/93‑1 (MQ=255)

ATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGC  >  NC_000913/1972200‑1972375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`