BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,973,518	A→T	D254E (GAT→GAA)	motB ←	MotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,973,518	0	A	T	99.1%	366.2 / NA	107	D254E (GAT→GAA)	motB	MotB
Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (66/39); minor base C (1/0); total (68/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

tGGCTTATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTCCCAGCAGGCTGATGCGACGGTTGACGGcttc                                                                                         <  2:1113001/93‑1 (MQ=255)

tGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttc                                                                                         <  2:1758663/93‑1 (MQ=255)

tGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttc                                                                                         >  1:253318/1‑93 (MQ=255)

tGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttc                                                                                         <  1:2197422/93‑1 (MQ=255)

  gCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttcat                                                                                       <  2:3077297/93‑1 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                      <  2:2853106/93‑1 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                     <  1:2505485/93‑1 (MQ=255)

     cATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAg                                                                                    >  2:914677/1‑93 (MQ=255)

      aTTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAgg                                                                                   <  2:515595/93‑1 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  <  2:95571/93‑1 (MQ=255)

        tCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTc                                                                                 <  2:7207/93‑1 (MQ=255)

        tCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTc                                                                                 <  2:3556125/93‑1 (MQ=255)

          tGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcg                                                                               <  2:4657912/93‑1 (MQ=255)

            gCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcgcg                                                                             >  1:967841/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                           >  2:277268/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                           >  2:4283678/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCCTCATCAGGTCCGCGAt                                                                           >  1:1714369/1‑93 (MQ=255)

                tCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCg                                                                         <  2:2474902/93‑1 (MQ=255)

                 cGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGc                                                                        >  1:2866258/1‑93 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                       <  1:49270/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                       >  1:3079186/1‑93 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGATGATGCGGCGGTTGACGGCTTCATCAGGTCCGCAATCGCt                                                                       >  2:3850716/1‑93 (MQ=255)

                   gCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCtt                                                                      >  2:347862/1‑93 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                     >  1:2637935/1‑93 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   >  1:3745444/1‑93 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   >  2:4479830/1‑93 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCACGTCCGCGGTCGCTTAAg                                                                   >  1:1786140/1‑93 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGGTCGCTTaacg                                                                  >  2:3546723/1‑91 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgc                                                                  >  2:3546724/1‑93 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgc                                                                  <  1:3586747/93‑1 (MQ=255)

                        ttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcg                                                                 >  2:1618306/1‑93 (MQ=255)

                          cATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCa                                                               >  1:2583599/1‑93 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                              >  2:1884805/1‑93 (MQ=255)

                            tGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATTAGGTCCGCGATCGCTTAAGCGCATc                                                             >  1:3615805/1‑93 (MQ=255)

                            tGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATc                                                             >  1:740613/1‑93 (MQ=255)

                             tcAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCg                                                            <  1:3659458/92‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                           >  1:2327696/1‑93 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          >  2:2203559/1‑93 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          >  2:4300761/1‑93 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          <  2:609426/93‑1 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          <  2:1134657/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        >  2:11554/1‑93 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        >  2:106221/1‑93 (MQ=255)

                                 aaTGGCCTGTTCGGCTCGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        >  1:1673615/1‑93 (MQ=255)

                                  aTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgcc                                                       >  1:3780569/1‑93 (MQ=255)

                                  aTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgcc                                                       >  1:2840159/1‑93 (MQ=255)

                                    ggCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgccgc                                                     <  1:498913/93‑1 (MQ=255)

                                    ggCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgccgc                                                     >  1:2479620/1‑93 (MQ=255)

                                      ccTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCa                                                   <  2:3354917/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                  <  2:3558079/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                  <  2:134039/93‑1 (MQ=255)

                                        tGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGACGCCATg                                                 >  1:1186544/1‑93 (MQ=255)

                                          ttCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGcc                                               >  1:1156285/1‑93 (MQ=255)

                                           tCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcg                                              >  1:1587393/1‑93 (MQ=255)

                                            tggCTTGTTTGTTCAGTACCAGCAGGCGGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  2:3264614/92‑1 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  1:4076211/93‑1 (MQ=255)

                                             ggCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgcc                                            >  1:3774698/1‑91 (MQ=255)

                                              gCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacg                                           >  1:3134324/1‑93 (MQ=255)

                                              gCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacg                                           >  1:3276947/1‑93 (MQ=255)

                                               cTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacga                                          >  2:2887573/1‑93 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         >  1:1200424/1‑93 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         >  2:4168784/1‑93 (MQ=255)

                                                 tgtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACTGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGaca                                        <  2:3296486/93‑1 (MQ=255)

                                                 tgtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGaca                                        >  1:2273887/1‑93 (MQ=255)

                                                   tttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACg                                      <  1:221295/93‑1 (MQ=255)

                                                   tttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACg                                      >  2:3256030/1‑93 (MQ=255)

                                                     tgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACAAGTa                                    >  1:3410254/1‑93 (MQ=255)

                                                        tCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAaca                                 >  2:4362139/1‑93 (MQ=255)

                                                          aGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACt                               >  2:1178198/1‑93 (MQ=255)

                                                          aGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACt                               <  1:1483449/93‑1 (MQ=255)

                                                           gTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACtt                              >  1:727529/1‑93 (MQ=255)

                                                           gTACCAGCAGGCGGATGCGACGGTTGAGGGCTGCAACAGGTCCGCGATCGCTTAAGCGCACCGTTGCCGCCATGCCGACGACACGTAACACtt                              <  1:1606335/93‑1 (MQ=255)

                                                             aCCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTg                            >  2:1221902/1‑93 (MQ=255)

                                                              ccAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGc                           <  1:466252/93‑1 (MQ=255)

                                                               cagcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGCCACGTAACACTTTGcc                          >  1:1271440/1‑93 (MQ=255)

                                                                agcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCg                         <  1:318090/93‑1 (MQ=255)

                                                                agcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCg                         <  1:3722173/93‑1 (MQ=255)

                                                                agcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCg                         >  1:2678679/1‑93 (MQ=255)

                                                                  cagGCTGAGGCGACGGTGTACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCt                       <  2:220807/93‑1 (MQ=255)

                                                                   agGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTa                      >  2:4307339/1‑93 (MQ=255)

                                                                   agGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTa                      >  2:3848843/1‑93 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                     >  1:1055933/1‑93 (MQ=255)

                                                                     gCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATc                    <  2:116840/93‑1 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                   <  1:3360911/93‑1 (MQ=255)

                                                                       tGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCa                  <  1:3139718/93‑1 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  1:3697417/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACAATTTGCCGCTATCCaa                 >  2:1868197/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGGACCACTTTGCCGCTATACac                 >  1:4540907/1‑92 (MQ=255)

                                                                         aTGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAc                <  1:783584/93‑1 (MQ=255)

                                                                         aTGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAc                >  1:1610684/1‑93 (MQ=255)

                                                                         aTGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAc                >  1:1589368/1‑93 (MQ=255)

                                                                          tGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAGCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAcc               <  2:3773239/93‑1 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              <  1:4043371/93‑1 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              <  2:1884232/93‑1 (MQ=255)

                                                                            cGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCt             <  2:1082276/93‑1 (MQ=255)

                                                                             gACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTCAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTc            >  2:4695108/1‑93 (MQ=255)

                                                                                    gACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTACCCAACCCTCCGAccct     >  2:2387284/1‑91 (MQ=255)

                                                                                       ggCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAg  >  1:996082/1‑93 (MQ=255)

                                                                                       ggCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAg  >  1:996083/1‑93 (MQ=255)

                                                                                       ggCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAAAACTTTGCCGCTATCCAACCCTCCGACCATCAg  >  1:731588/1‑93 (MQ=255)

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`