BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,013,946	G→A	Q26Q (CAG→CAA)	fliH →	FliH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,013,946	0	G	A	97.6%	426.3 / ‑3.0	124	Q26Q (CAG→CAA)	fliH	FliH
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (60/61); total (62/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 128 total aligned reads displayed.

GCGAGATGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAAT  >  NC_000913/2013857‑2014035

  gagaTGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCaga                                                                                      <  1:1067130/93‑1 (MQ=255)

  gagaTGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCaga                                                                                      >  2:429726/1‑93 (MQ=255)

  gagaTGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCaga                                                                                      >  1:1957852/1‑93 (MQ=255)

   agaTGGTAATTGGCAGCGGCGAGGATACCTATGTCGGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCagag                                                                                     >  1:837363/1‑93 (MQ=255)

    gaTGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGt                                                                                    >  1:4266829/1‑93 (MQ=255)

     aTGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGtt                                                                                   <  2:1037846/93‑1 (MQ=255)

      tGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGttt                                                                                  <  1:2432689/93‑1 (MQ=255)

       ggTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTtg                                                                                 <  2:1390095/93‑1 (MQ=255)

       ggTAATTGGCAGCGGCGAAGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTtg                                                                                 >  1:2452452/1‑93 (MQ=255)

        gTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTtgt                                                                                <  2:225556/93‑1 (MQ=255)

        gTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTtgt                                                                                <  1:4518551/93‑1 (MQ=255)

         tAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTtgtg                                                                               >  1:2614370/1‑93 (MQ=255)

           aTTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGcc                                                                             <  1:2176072/93‑1 (MQ=255)

           aTTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGcc                                                                             <  1:4563310/93‑1 (MQ=255)

            ttGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGccc                                                                            >  2:3461712/1‑93 (MQ=255)

              ggCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCAt                                                                          <  1:1565251/93‑1 (MQ=255)

                 cgcggcgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGt                                                                       <  1:1788607/92‑1 (MQ=255)

                 aGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGt                                                                       <  2:2764747/93‑1 (MQ=255)

                 aGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGt                                                                       <  2:3201097/93‑1 (MQ=255)

                  gcggcgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTc                                                                      >  1:2256185/1‑93 (MQ=255)

                    ggcgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCgg                                                                    >  2:3341964/1‑92 (MQ=255)

                    ggcgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGa                                                                    <  1:3121124/93‑1 (MQ=255)

                     gcgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAg                                                                   <  2:1170833/93‑1 (MQ=255)

                      cgAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGc                                                                  <  1:1299538/93‑1 (MQ=255)

                       gAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGcc                                                                 >  2:1758925/1‑93 (MQ=255)

                        aGGATGCCTATGTCTGATACTCTGCCGTGGAACACCTGGACGCCGGACGTTCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGGGCCg                                                                <  2:3609250/93‑1 (MQ=255)

                        aGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCg                                                                >  2:2964959/1‑93 (MQ=255)

                        aGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCg                                                                >  1:1061865/1‑93 (MQ=255)

                        aGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCg                                                                >  1:2287969/1‑93 (MQ=255)

                          gATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGga                                                              >  2:1757651/1‑93 (MQ=255)

                             aCCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGgaaga                                                           >  2:201859/1‑93 (MQ=255)

                             aCCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGgaaga                                                           >  1:2383825/1‑93 (MQ=255)

                               cTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGaaa                                                         >  1:1088720/1‑93 (MQ=255)

                               cTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGaaa                                                         <  2:1673638/93‑1 (MQ=255)

                               cTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGaaa                                                         >  1:52857/1‑93 (MQ=255)

                                tATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAAc                                                        >  2:866804/1‑93 (MQ=255)

                                tATGTCTGATAATCTGCAGTGGAAAACCTGGACGCCGGACGATCTCGCGCCTCGACAGGCAGAGATTGAGCCCATAGTAGAGCCGGAAGCAAc                                                        >  2:2765103/1‑93 (MQ=255)

                                aaTGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAAc                                                        <  1:2138140/92‑1 (MQ=255)

                                  tGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACca                                                      >  1:2846575/1‑93 (MQ=255)

                                  tGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACca                                                      >  1:2242118/1‑93 (MQ=255)

                                  tGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATATCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACca                                                      >  2:1281635/1‑93 (MQ=255)

                                   gTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcat                                                     >  1:488647/1‑93 (MQ=255)

                                   gTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcat                                                     >  1:488648/1‑93 (MQ=255)

                                   gTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcat                                                     <  2:2639153/93‑1 (MQ=255)

                                    tCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcatc                                                    <  2:3402641/93‑1 (MQ=255)

                                    tCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcatc                                                    >  2:4183240/1‑93 (MQ=255)

                                    tCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcatc                                                    <  2:568748/93‑1 (MQ=255)

                                      tGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGACGAGCCGGAAGAAACcatcat                                                  >  1:3995917/1‑93 (MQ=255)

                                      tGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACcatcat                                                  >  2:1822329/1‑93 (MQ=255)

                                       gATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCTCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCAtt                                                 >  1:3623875/1‑93 (MQ=255)

                                       gATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCAtt                                                 >  1:259800/1‑93 (MQ=255)

                                          aaTCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGaa                                              >  2:3131698/1‑93 (MQ=255)

                                          aaTCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGaa                                              >  2:2512039/1‑93 (MQ=255)

                                            tCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAaga                                            >  2:4620046/1‑93 (MQ=255)

                                            tCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAaga                                            <  1:3609702/93‑1 (MQ=255)

                                            tCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAaga                                            <  1:2307691/93‑1 (MQ=255)

                                             cTGCCGTGGAAAACCTGGACGCCGGACGAACTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAagag                                           <  2:1703111/93‑1 (MQ=255)

                                              tGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAgg                                          >  1:947834/1‑93 (MQ=255)

                                              tGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAgg                                          >  1:2835583/1‑93 (MQ=255)

                                              cgCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAgg                                          <  2:3534172/92‑1 (MQ=255)

                                               gCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGc                                         <  1:866606/93‑1 (MQ=255)

                                                  gTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGa                                      >  1:1769486/1‑93 (MQ=255)

                                                  gTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGa                                      <  1:1644149/93‑1 (MQ=255)

                                                   tGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGaa                                     >  1:3775076/1‑93 (MQ=255)

                                                     gAAAACCTGGACGCCGGACGATCTCGCGCCACCACAATCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAAcc                                   >  2:3479981/1‑93 (MQ=255)

                                                     gAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAAcc                                   >  2:1389823/1‑93 (MQ=255)

                                                     gAAAACCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAAcc                                   >  1:3464317/1‑93 (MQ=255)

                                                       aaaCCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCa                                 <  1:3779049/93‑1 (MQ=255)

                                                       aaaCCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCa                                 >  2:676911/1‑93 (MQ=255)

                                                       aaaCCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCa                                 <  2:838795/93‑1 (MQ=255)

                                                        aaCCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAg                                <  2:3121880/93‑1 (MQ=255)

                                                         aCCTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGc                               <  1:3386147/93‑1 (MQ=255)

                                                           cTGGACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCt                             <  2:2154870/93‑1 (MQ=255)

                                                             ggACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTg                           <  2:54090/93‑1 (MQ=255)

                                                             ggACGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTg                           >  1:3772748/1‑93 (MQ=255)

                                                               aCGCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGag                         <  2:4580393/93‑1 (MQ=255)

                                                                 gcgggACGACCTCGCGCCACCACAAGCAGAGTTTGTGCCCAAAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGagca                       <  2:1246444/90‑1 (MQ=255)

                                                                 gCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGagca                       <  1:464091/93‑1 (MQ=255)

                                                                 gCCGGACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGagca                       >  1:1565359/1‑93 (MQ=255)

                                                                    ggACGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAgcagca                    >  1:4320476/1‑93 (MQ=255)

                                                                      aCGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAAc                  <  2:412157/93‑1 (MQ=255)

                                                                       cGATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACt                 <  2:3523393/93‑1 (MQ=255)

                                                                        gATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTg                <  2:2112828/93‑1 (MQ=255)

                                                                        gATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTg                <  2:2085047/93‑1 (MQ=255)

                                                                        gATCTCGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTg                <  2:263176/93‑1 (MQ=255)

                                                                          tctcGCGCCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGgc              >  1:40504/1‑93 (MQ=255)

                                                                              gcgcCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCaa          <  2:2233890/93‑1 (MQ=255)

                                                                               cgcCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAAc         <  1:1625757/93‑1 (MQ=255)

                                                                               cgcCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAAc         <  2:2059595/93‑1 (MQ=255)

                                                                                gcCACCACAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACt        <  2:834889/93‑1 (MQ=255)

                                                                                 ccaccaCAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGCACCCAGCCTTGAACAGCAACTGGCGCAACtc       >  2:1201987/1‑92 (MQ=255)

                                                                                 ccaccaCAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTg       >  2:2663913/1‑93 (MQ=255)

                                                                                 ccaccaCAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTg       <  2:4720715/93‑1 (MQ=255)

                                                                                  caccacAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGc      >  2:2719256/1‑93 (MQ=255)

                                                                                   accacAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCa     >  1:4399248/1‑93 (MQ=255)

                                                                                   accacAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCa     >  2:1024501/1‑93 (MQ=255)

                                                                                   accacAAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCa     >  1:875939/1‑93 (MQ=255)

                                                                                     cacaGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCaaa   >  2:3995363/1‑93 (MQ=255)

                                                                                     cacaAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCaaa   <  1:3362656/93‑1 (MQ=255)

                                                                                      acaAGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAAt  <  2:4050631/93‑1 (MQ=255)

GCGAGATGGTAATTGGCAGCGGCGAGGATACCTATGTCTGATAATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAAT  >  NC_000913/2013857‑2014035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`