BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,026,680	T→C	T237A (ACG→GCG)	yedI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,026,680	0	T	C	93.5%	343.3 / 8.9	123	T237A (ACG→GCG)	yedI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/6); new base C (39/76); total (41/82)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 130 total aligned reads displayed.

TTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTC  >  NC_000913/2026590‑2026767

tttGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAgcgcgc                                                                                       >  2:4045544/1‑93 (MQ=255)

 ttGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGcc                                                                                      <  2:1193440/93‑1 (MQ=255)

    ctgcCCGGCGAAATGTTCAATGGCGTGCTGCAGCGGCGCCATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGaa                                                                                   >  2:1422955/1‑92 (MQ=255)

      gcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACaa                                                                                 >  1:4509532/1‑93 (MQ=255)

         cGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCg                                                                              <  2:1786905/93‑1 (MQ=255)

            cGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATa                                                                           <  2:3781813/93‑1 (MQ=255)

             gAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATaa                                                                          >  2:3207275/1‑93 (MQ=255)

             gAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATaa                                                                          <  1:3668363/93‑1 (MQ=255)

             gAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATaa                                                                          <  2:1662552/93‑1 (MQ=255)

             gAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATaa                                                                          <  1:2253313/93‑1 (MQ=255)

              aaaTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAAc                                                                         <  2:311115/93‑1 (MQ=255)

               aaTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGCTAACg                                                                        >  1:178902/1‑93 (MQ=255)

               aaTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACg                                                                        >  1:2629902/1‑93 (MQ=255)

                aTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCGCCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGc                                                                       <  1:522926/93‑1 (MQ=255)

                aTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGc                                                                       >  2:3507311/1‑93 (MQ=255)

                aTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGc                                                                       <  2:2051153/93‑1 (MQ=255)

                 tGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCt                                                                      <  1:1432357/93‑1 (MQ=255)

                 tGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCt                                                                      >  2:2054217/1‑93 (MQ=255)

                    tCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtc                                                                   >  1:1656848/1‑93 (MQ=255)

                    tCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtc                                                                   <  2:296818/93‑1 (MQ=255)

                    tCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtc                                                                   >  1:2932900/1‑93 (MQ=255)

                     cAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtca                                                                  <  2:1398760/93‑1 (MQ=255)

                      agtGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcat                                                                 <  2:691807/91‑1 (MQ=255)

                      aaTGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcat                                                                 >  1:2808851/1‑93 (MQ=255)

                       aTGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcatc                                                                >  1:1431147/1‑93 (MQ=255)

                       aTGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcatc                                                                <  1:2144342/93‑1 (MQ=255)

                       aTGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcatc                                                                >  2:4332253/1‑93 (MQ=255)

                        tGGCGTGATGCAGCGGCGCACTTCCATGCACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGTCACTAAATAACGCTTtaatca                                                               >  2:2538290/1‑93 (MQ=255)

                        tGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcatca                                                               <  1:2552001/93‑1 (MQ=255)

                        tGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTtcatca                                                               >  1:4382871/1‑93 (MQ=255)

                          gCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGc                                                             <  1:373652/93‑1 (MQ=255)

                           cGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGcg                                                            <  1:3561574/93‑2 (MQ=255)

                            gTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCa                                                           <  1:762679/93‑1 (MQ=255)

                            gTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCa                                                           >  1:4189262/1‑93 (MQ=255)

                             tGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAg                                                          <  2:4266374/93‑1 (MQ=255)

                             tGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAg                                                          <  2:2540587/93‑1 (MQ=255)

                              gATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAgg                                                         <  1:2120765/93‑1 (MQ=255)

                                tGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGgc                                                       <  2:3356174/93‑1 (MQ=255)

                                 gCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGgcg                                                      <  2:554459/93‑1 (MQ=255)

                                 gCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGgcg                                                      <  1:403645/93‑1 (MQ=255)

                                   aGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGcgcg                                                    >  2:936151/1‑93 (MQ=255)

                                     gggcgCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGAt                                                  <  1:4463545/92‑1 (MQ=255)

                                     cggcgCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGAt                                                  <  2:1757409/93‑1 (MQ=255)

                                       gcgcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATaa                                                <  2:431118/93‑1 (MQ=255)

                                        cgcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAAt                                               <  2:3785815/93‑1 (MQ=255)

                                         gcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATc                                              <  1:1318799/93‑1 (MQ=255)

                                         gcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATc                                              <  2:3119450/93‑1 (MQ=255)

                                           aaTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCaa                                            >  2:1133483/1‑93 (MQ=255)

                                           aaTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCaa                                            <  1:4427882/93‑1 (MQ=255)

                                           aaTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCaa                                            >  1:3983834/1‑93 (MQ=255)

                                            aTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAAt                                           >  1:1521585/1‑93 (MQ=255)

                                            aTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAAt                                           <  1:2630026/93‑1 (MQ=255)

                                            aTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAAt                                           >  2:2736771/1‑93 (MQ=255)

                                             ttCGATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATa                                          <  2:258039/93‑1 (MQ=255)

                                              tCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCCATAACGCTTTCATCAGCCAGAGCGCGATAAACAATaa                                         >  2:2193668/1‑93 (MQ=255)

                                               ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAc                                        <  1:3051816/93‑1 (MQ=255)

                                               ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAc                                        <  1:241688/93‑1 (MQ=255)

                                                cATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAcc                                       <  2:572434/93‑1 (MQ=255)

                                                 aTGTCCCCCAATCCCCCCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAccc                                      <  2:919592/93‑1 (MQ=255)

                                                 aTGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAccc                                      <  2:919740/93‑1 (MQ=255)

                                                 aTGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAccc                                      >  2:2693199/1‑93 (MQ=255)

                                                       cacaATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTAcc                                <  2:3770682/93‑1 (MQ=255)

                                                        ccaATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt                               <  2:4104534/92‑1 (MQ=255)

                                                        acaATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt                               >  2:2434174/1‑93 (MQ=255)

                                                        acaATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt                               <  1:1327972/93‑1 (MQ=255)

                                                        acaATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt                               <  1:2271971/93‑1 (MQ=255)

                                                         caATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTa                              <  1:2453489/93‑1 (MQ=255)

                                                         caATCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTa                              <  2:1560322/93‑1 (MQ=255)

                                                          aaTCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTaa                             <  2:2679302/93‑1 (MQ=255)

                                                          aaTCCAACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTaa                             <  1:4345755/93‑1 (MQ=255)

                                                           aTCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAAt                            >  2:215663/1‑93 (MQ=255)

                                                           aTCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAAt                            <  2:3600472/93‑1 (MQ=255)

                                                           aTCCCACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAAt                            <  1:3515295/93‑1 (MQ=255)

                                                              cccccgccgACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                         <  1:2424838/90‑1 (MQ=255)

                                                              ccACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                         <  2:3715878/93‑1 (MQ=255)

                                                              ccACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                         >  1:1641062/1‑93 (MQ=255)

                                                              ccACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                         <  1:4067242/93‑1 (MQ=255)

                                                              ccACCGCCGACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                         >  2:1978433/1‑93 (MQ=255)

                                                                cccgccgACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTg                       <  1:4099464/92‑1 (MQ=255)

                                                                   gccgACGAGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCAt                    <  2:250325/93‑1 (MQ=255)

                                                                     cgacgaGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCa                  >  2:2046383/1‑93 (MQ=255)

                                                                     cgacgaGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCa                  >  2:2485828/1‑93 (MQ=255)

                                                                      gacgaGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAg                 >  1:4693426/1‑93 (MQ=255)

                                                                      aacgaGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAg                 <  2:2264431/92‑1 (MQ=255)

                                                                       acgaGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAgc                <  2:3053259/93‑1 (MQ=255)

                                                                         gaGGAACATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAgcgc              >  2:839119/1‑93 (MQ=255)

                                                                             aaCATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTg          <  2:4043265/93‑1 (MQ=255)

                                                                              aCATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTgg         <  1:1538464/93‑1 (MQ=255)

                                                                               cATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATATCCCTTTACCTAATGCCTGCATCAGCGCGCTGGa        <  2:2150989/93‑1 (MQ=255)

                                                                               cATCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGa        <  1:2913806/93‑1 (MQ=255)

                                                                                aTCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTCCCTAATGCCTGCATCAGCGCGCTGGAt       >  2:593018/1‑93 (MQ=255)

                                                                                aTCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAt       >  2:593019/1‑93 (MQ=255)

                                                                                aTCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAt       <  2:3205648/93‑1 (MQ=255)

                                                                                aTCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAt       >  2:694228/1‑93 (MQ=255)

                                                                                aTCGCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAt       >  1:3645841/1‑93 (MQ=255)

                                                                                   gCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAtttt    >  1:3600439/1‑93 (MQ=255)

                                                                                   gCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAtttt    <  2:1162075/93‑1 (MQ=255)

                                                                                   gCCAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAtttt    <  1:4662692/93‑1 (MQ=255)

                                                                                     cAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTc  <  1:1546956/93‑1 (MQ=255)

                                                                                     cAGCGCGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTc  <  2:3651567/93‑1 (MQ=255)

TTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTC  >  NC_000913/2026590‑2026767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`