BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,069,029	T→C	S169S (AGT→AGC)	yeeP →	putative histone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,069,029	0	T	C	98.5%	429.3 / ‑2.6	137	S169S (AGT→AGC)	yeeP	putative histone
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (74/61); total (75/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-01

Only 100 of 141 total aligned reads displayed.

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

 ccGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGcccc                                                                                        >  2:456044/1‑93 (MQ=255)

       gtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                  <  2:3334003/93‑1 (MQ=255)

         ggCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAc                                                                                >  2:4299590/1‑93 (MQ=255)

            cGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCg                                                                             <  1:1541340/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTTGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  1:3387640/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  2:2920206/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  1:1943151/93‑1 (MQ=255)

               aTCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACt                                                                          >  2:429514/1‑93 (MQ=255)

               aTCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACt                                                                          <  1:2524745/93‑1 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  1:3616215/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  1:4242092/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  2:1820518/1‑93 (MQ=255)

                 cGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGc                                                                        >  1:276824/1‑93 (MQ=255)

                 cGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGc                                                                        <  2:1772418/93‑1 (MQ=255)

                   gCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAg                                                                      <  1:2891791/93‑1 (MQ=255)

                    cccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAgg                                                                     <  1:2879942/93‑1 (MQ=255)

                      cGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAc                                                                   <  2:1678464/93‑1 (MQ=255)

                      cGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAc                                                                   >  1:4569633/1‑93 (MQ=255)

                      cGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAc                                                                   <  2:3758718/93‑1 (MQ=255)

                         aCCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAg                                                                >  2:1609811/1‑93 (MQ=255)

                          ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                               >  2:1701561/1‑93 (MQ=255)

                          ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                               >  1:3080822/1‑93 (MQ=255)

                           cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                              >  1:4254439/1‑93 (MQ=255)

                           cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                              >  1:4519411/1‑93 (MQ=255)

                            ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                             >  2:1912647/1‑93 (MQ=255)

                             gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                            <  2:3473337/93‑1 (MQ=255)

                             gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                            <  2:3666020/93‑1 (MQ=255)

                               tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                          >  2:2662059/1‑93 (MQ=255)

                               tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                          <  2:3088029/93‑1 (MQ=255)

                                 ggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAc                                                        >  2:3522002/1‑93 (MQ=255)

                                    aCTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGa                                                     >  2:3004069/1‑93 (MQ=255)

                                     cTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGaa                                                    <  1:1430253/93‑1 (MQ=255)

                                     cTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGaa                                                    <  2:2206749/93‑1 (MQ=255)

                                      tGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGCCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGATGCGCACGGAAt                                                   >  2:763129/1‑93 (MQ=255)

                                      tGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAAt                                                   >  2:763128/1‑93 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  <  2:4111793/93‑1 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  >  1:4174067/1‑93 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  >  1:2206890/1‑93 (MQ=255)

                                        gATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCt                                                 <  1:3584873/93‑1 (MQ=255)

                                        gATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCt                                                 <  2:1398271/93‑1 (MQ=255)

                                        gATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCt                                                 >  2:1602772/1‑93 (MQ=255)

                                           aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                              >  2:3391382/1‑93 (MQ=255)

                                           aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                              >  1:4665384/1‑93 (MQ=255)

                                           aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                              <  1:955780/93‑1 (MQ=255)

                                            cGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCTCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTcc                                             <  2:3140691/93‑1 (MQ=255)

                                             gCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCg                                            >  2:3510508/1‑93 (MQ=255)

                                              cTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCgg                                           >  1:1762676/1‑93 (MQ=255)

                                               tGGTCGGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCggc                                          >  2:3966866/1‑92 (MQ=255)

                                                ggTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtc                                         >  1:3381681/1‑93 (MQ=255)

                                                 gTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        <  1:2006009/93‑1 (MQ=255)

                                                 gTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        <  2:381461/93‑1 (MQ=255)

                                                  tCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtctc                                       <  1:1262616/93‑1 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  2:2228678/93‑1 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  2:938484/93‑1 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      >  1:1046347/1‑93 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  1:3513036/93‑1 (MQ=255)

                                                     gTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAgg                                    >  2:119847/1‑93 (MQ=255)

                                                       gcgcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGcc                                  <  1:3214939/93‑1 (MQ=255)

                                                        cgcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGccc                                 >  1:1137193/1‑93 (MQ=255)

                                                         gcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCg                                >  2:3550501/1‑93 (MQ=255)

                                                          cTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGt                               >  1:4589260/1‑93 (MQ=255)

                                                          cTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGt                               <  1:2138747/93‑1 (MQ=255)

                                                             aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                            >  2:4667043/1‑93 (MQ=255)

                                                             aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                            >  2:580088/1‑93 (MQ=255)

                                                             aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                            >  1:2330172/1‑93 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  1:1930346/93‑1 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  2:4026008/93‑1 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  2:2043404/93‑1 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         >  1:2798404/1‑93 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTACGGTCTCAGGCCCGTGAACAg                         >  2:1148456/1‑93 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       >  2:1914680/1‑93 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       <  1:3393424/93‑1 (MQ=255)

                                                                    cgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTa                     <  2:389683/93‑1 (MQ=255)

                                                                     gcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAc                    <  1:4400774/93‑1 (MQ=255)

                                                                      cTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAcc                   >  1:2852058/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  2:3496379/93‑1 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  2:3763899/93‑1 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  2:2104911/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 >  1:2410562/1‑93 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 >  1:3677997/1‑93 (MQ=255)

                                                                         cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt                >  2:1673507/1‑93 (MQ=255)

                                                                         cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt                <  2:4114229/93‑1 (MQ=255)

                                                                          ccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtg               >  2:598975/1‑93 (MQ=255)

                                                                          ccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtg               >  2:90942/1‑93 (MQ=255)

                                                                           cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgc              >  1:2626593/1‑93 (MQ=255)

                                                                            gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg             >  2:2216392/1‑93 (MQ=255)

                                                                            gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg             >  2:4201603/1‑93 (MQ=255)

                                                                            gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg             >  2:4286642/1‑93 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            <  1:554029/93‑1 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            >  1:4482350/1‑93 (MQ=255)

                                                                                aTGCCGCCAGCCCCCTGATGACCCGACTGTAGGACGAGCTGCGCACGGAATCTGTCGGGTCTCCGGCCCGTGAAGAGTTTACCCGTGCGGTgg         >  1:1011498/1‑93 (MQ=255)

                                                                                aTGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTgg         >  1:2279145/1‑93 (MQ=255)

                                                                                aTGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTgg         >  1:1011499/1‑93 (MQ=255)

                                                                                 tGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGa        >  1:3307575/1‑93 (MQ=255)

                                                                                 tGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGa        >  1:4311910/1‑93 (MQ=255)

                                                                                  gccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAc       <  2:399657/93‑1 (MQ=255)

                                                                                    cgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCg     >  1:1810732/1‑93 (MQ=255)

                                                                                     gccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCgg    <  2:3593289/93‑1 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGa   <  1:1497011/93‑1 (MQ=255)

                                                                                       cAGCCCCCTGATGACCCGACTGCAGGACGATCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGat  >  2:2743068/1‑93 (MQ=255)

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`