BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,089,888	T→C	L257P (CTC→CCC)	hisD →	L‑histidinal:NAD+ oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,888	0	T	C	100.0%	351.3 / NA	108	L257P (CTC→CCC)	hisD	L‑histidinal:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (48/60); total (48/60)

Only 100 of 115 total aligned reads displayed.

GCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAA  >  NC_000913/2089800‑2089976

gcggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGtcctct                                                                                      >  2:2025328/1‑93 (MQ=255)

gcggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctct                                                                                      <  1:1036063/93‑1 (MQ=255)

 cggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCcctctc                                                                                     >  1:2165020/1‑93 (MQ=255)

   gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                   >  1:2503694/1‑93 (MQ=255)

     gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                 >  2:3926149/1‑93 (MQ=255)

     gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                 >  1:2198886/1‑93 (MQ=255)

      atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                >  2:1535161/1‑93 (MQ=255)

      atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                <  1:300425/93‑1 (MQ=255)

       tcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTg                                                                               >  2:3018828/1‑93 (MQ=255)

       tcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTg                                                                               >  1:987156/1‑93 (MQ=255)

        cgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGa                                                                              <  1:496086/93‑1 (MQ=255)

        cgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGa                                                                              >  2:684097/1‑93 (MQ=255)

         gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                             <  2:4343875/93‑1 (MQ=255)

         gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                             <  1:3151365/93‑1 (MQ=255)

         gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                             <  2:1137065/93‑1 (MQ=255)

          atatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAac                                                                            <  1:647449/93‑1 (MQ=255)

           tatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTTAGGCTGaaac                                                                           >  2:2931983/1‑91 (MQ=255)

           tatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAaca                                                                           >  2:4471829/1‑93 (MQ=255)

           tatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAaca                                                                           >  1:3499984/1‑93 (MQ=255)

           tatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAaca                                                                           <  2:3398831/93‑1 (MQ=255)

            atGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAacac                                                                          <  1:3527411/93‑1 (MQ=255)

            atGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAacac                                                                          <  2:3272435/93‑1 (MQ=255)

            atGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAacac                                                                          <  1:486583/93‑1 (MQ=255)

               cccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGc                                                                       <  2:3583079/93‑1 (MQ=255)

                ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                      >  1:4389548/1‑93 (MQ=255)

                ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                      >  1:3412284/1‑93 (MQ=255)

                ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                      >  2:3581837/1‑93 (MQ=255)

                 cGCAGGCCCGTCGGAGGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCACAGGCTGAACACGGacc                                                                     <  2:4214802/93‑1 (MQ=255)

                 cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                     >  1:1070379/1‑93 (MQ=255)

                 cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                     <  1:3433310/93‑1 (MQ=255)

                 cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                     <  2:9154/93‑1 (MQ=255)

                  gCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCg                                                                    <  2:1942840/93‑1 (MQ=255)

                    aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGa                                                                  >  2:2368978/1‑93 (MQ=255)

                    aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACAAGGCCCggc                                                                  >  1:1475813/1‑92 (MQ=255)

                     ggCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAc                                                                 >  1:1014666/1‑93 (MQ=255)

                      gCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACt                                                                >  2:700539/1‑93 (MQ=255)

                      gCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACt                                                                <  2:2589394/93‑1 (MQ=255)

                       cccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTc                                                               <  2:1722981/93‑1 (MQ=255)

                          gTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcaca                                                            <  2:2824761/93‑1 (MQ=255)

                           tgtgAAGTGCTGGTGATGGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                           <  1:1490656/90‑1 (MQ=255)

                           tCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                           >  1:329207/1‑93 (MQ=255)

                           tCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                           <  2:4728271/93‑1 (MQ=255)

                            cGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAgg                                                          >  1:2720297/1‑93 (MQ=255)

                             ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAggg                                                         >  2:4292528/1‑92 (MQ=255)

                              gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGCGTCACAGGTg                                                        >  1:3527566/1‑93 (MQ=255)

                              gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                        >  2:2473690/1‑93 (MQ=255)

                              gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                        <  2:2630897/93‑1 (MQ=255)

                              gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                        >  2:656166/1‑93 (MQ=255)

                               aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                       <  2:4737113/93‑1 (MQ=255)

                               aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                       <  2:4737112/93‑1 (MQ=255)

                               aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                       >  1:1944595/1‑93 (MQ=255)

                                aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                      <  2:1438409/93‑1 (MQ=255)

                                  tGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                    >  2:1232910/1‑93 (MQ=255)

                                   gCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAtttt                                                   <  2:3968907/93‑1 (MQ=255)

                                    cTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTa                                                  <  2:2017638/93‑1 (MQ=255)

                                    cTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGATTCTGATTTGCTCTCTCAGGCTGTAGACGGCCCGGACTCACAGGAGATTTTa                                                  >  2:4232796/1‑93 (MQ=255)

                                      ggTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACt                                                <  2:1566172/93‑1 (MQ=255)

                                      ggTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACt                                                <  1:2382280/93‑1 (MQ=255)

                                          aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCCCAGGTGATTTTACTGAcc                                            >  2:269499/1‑92 (MQ=255)

                                          aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAcc                                            >  1:2565657/1‑92 (MQ=255)

                                           ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACCGGTGATTTTACTGCCGc                                           >  1:2741739/1‑93 (MQ=255)

                                           ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGc                                           >  2:2204167/1‑93 (MQ=255)

                                            tGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGcc                                          >  1:4106619/1‑93 (MQ=255)

                                                gACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgct                                      <  1:3167483/93‑1 (MQ=255)

                                                   aGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCgctgct                                   >  2:2005633/1‑93 (MQ=255)

                                                    gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGctgctg                                  <  2:2797611/93‑1 (MQ=255)

                                                     cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                 <  1:2875174/93‑1 (MQ=255)

                                                       gcgcTACGCCTGATTGCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGata                               <  1:4493624/93‑1 (MQ=255)

                                                       gcgcTACGCCGTATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGGCTCACAGGTGATTTTACTGACGCCCGCTGCTGata                               <  2:1952525/93‑1 (MQ=255)

                                                       gcgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGata                               <  1:119651/93‑1 (MQ=255)

                                                         gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGGGATTTTACTGACGCCCGCTGCTGATATg                             >  1:1373803/1‑93 (MQ=255)

                                                           tACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgc                           <  2:2635214/93‑1 (MQ=255)

                                                           tACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgc                           >  2:28009/1‑93 (MQ=255)

                                                            aCGCCTGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgcg                          <  2:941788/93‑1 (MQ=255)

                                                              gCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcg                        >  1:3835845/1‑93 (MQ=255)

                                                                cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                      >  2:2383299/1‑93 (MQ=255)

                                                                 ggATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcg                     >  1:1983033/1‑93 (MQ=255)

                                                                    tttCGTTGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                  <  1:2756738/93‑1 (MQ=255)

                                                                     ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                 <  1:2753499/93‑1 (MQ=255)

                                                                     ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                 <  1:2415941/93‑1 (MQ=255)

                                                                     ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                 <  1:349070/93‑1 (MQ=255)

                                                                       cGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGc               <  1:3794973/93‑1 (MQ=255)

                                                                        gTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGcc              >  1:872171/1‑93 (MQ=255)

                                                                         tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg             <  2:1182735/93‑1 (MQ=255)

                                                                         tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg             >  1:1038774/1‑93 (MQ=255)

                                                                         tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg             >  1:1342108/1‑93 (MQ=255)

                                                                          ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa            <  1:4113352/93‑1 (MQ=255)

                                                                          ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa            >  1:4479595/1‑93 (MQ=255)

                                                                            cTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAgg          >  2:854369/1‑93 (MQ=255)

                                                                             ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc         <  1:3793124/93‑1 (MQ=255)

                                                                             ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc         >  2:3045245/1‑93 (MQ=255)

                                                                              tCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGcc        <  2:3026435/93‑1 (MQ=255)

                                                                                tGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGt      <  1:1467318/93‑1 (MQ=255)

                                                                                tGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGt      <  2:3332685/93‑1 (MQ=255)

                                                                                 gATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGGCCAGGCCGTc     >  2:3237286/1‑93 (MQ=255)

                                                                                 gATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTc     <  2:4713104/93‑1 (MQ=255)

                                                                                  aTTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCg    <  2:4295708/93‑1 (MQ=255)

                                                                                    ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa  <  2:3717907/93‑1 (MQ=255)

                                                                                    ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa  <  1:3502510/93‑1 (MQ=255)

                                                                                    ttGCCCTCTCAGGCTGAACACGACCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa  <  2:453850/93‑1 (MQ=255)

GCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAA  >  NC_000913/2089800‑2089976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`