Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,108,194 T→C Q283R (CAG→CGG)  rfbA ← glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,108,1940TC99.4% 583.7 / ‑4.9 178Q283R (CAG→CGG) rfbAglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (84/93);  total (84/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 181 total aligned reads displayed.

AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283
                                                                                          |                                                                                         
    aCTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCAt                                                                                     <  1:4209791/93‑1 (MQ=255)
          ggCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattat                                                                               >  1:2958602/1‑93 (MQ=255)
          ggCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattat                                                                               <  1:2474649/93‑1 (MQ=255)
           gCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattatt                                                                              <  2:3684409/93‑1 (MQ=255)
             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  2:4533797/93‑1 (MQ=255)
             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            >  2:132763/1‑93 (MQ=255)
             tCCAGAATTAGCACACCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  1:844233/93‑1 (MQ=255)
               cAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCttt                                                                          >  2:3222992/1‑93 (MQ=255)
               cAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCttt                                                                          >  1:1296663/1‑93 (MQ=255)
                aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         <  2:377484/93‑1 (MQ=255)
                aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         >  2:2212879/1‑93 (MQ=255)
                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  1:2557950/1‑93 (MQ=255)
                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:4265550/1‑93 (MQ=255)
                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  1:2967190/1‑93 (MQ=255)
                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:1987430/1‑93 (MQ=255)
                  aaTTAGCACATCTTCAATTTCCGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  1:4230243/1‑93 (MQ=255)
                  aaTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  2:1816442/1‑93 (MQ=255)
                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      <  2:3393594/93‑1 (MQ=255)
                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      >  1:516042/1‑93 (MQ=255)
                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      <  1:4699907/93‑1 (MQ=255)
                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    <  1:3636856/93‑1 (MQ=255)
                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   >  2:721600/1‑93 (MQ=255)
                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   >  2:1373499/1‑93 (MQ=255)
                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   <  2:4312624/93‑1 (MQ=255)
                        cacaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 <  1:3566885/93‑1 (MQ=255)
                        cacaTCTTCAATTTCAGTGCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 <  2:3005273/93‑1 (MQ=255)
                         acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                <  1:3403670/93‑1 (MQ=255)
                           ctCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              <  2:2138808/92‑1 (MQ=255)
                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              <  1:2948377/93‑1 (MQ=255)
                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              >  2:3769928/1‑93 (MQ=255)
                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              >  1:3689216/1‑93 (MQ=255)
                            tCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAg                                                             >  2:2688950/1‑93 (MQ=255)
                            tCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAg                                                             >  2:4157192/1‑93 (MQ=255)
                               tCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTa                                                          >  2:2651494/1‑93 (MQ=255)
                                cAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTaa                                                         <  1:2095797/93‑1 (MQ=255)
                                  aTTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAtt                                                       >  2:1093439/1‑93 (MQ=255)
                                   tttCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAttt                                                      >  1:1175082/1‑93 (MQ=255)
                                       aGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCtt                                                  <  2:69111/93‑1 (MQ=255)
                                        gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 >  2:1348568/1‑93 (MQ=255)
                                        gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 <  2:644832/93‑1 (MQ=255)
                                         ttCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTAc                                                >  2:3620606/1‑93 (MQ=255)
                                          tCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACt                                               <  2:3311895/93‑1 (MQ=255)
                                           cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              <  2:2952149/93‑1 (MQ=255)
                                             aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            >  1:1346163/1‑93 (MQ=255)
                                             aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            >  1:4357117/1‑93 (MQ=255)
                                              aTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCt                                           <  2:4140100/93‑1 (MQ=255)
                                               tCACATTCGTTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAGTTGTTCTTTATTACTGGTACAGCTAATGTTCTTACTTGCtt                                          >  2:1926974/1‑92 (MQ=255)
                                               tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  2:1054223/1‑93 (MQ=255)
                                               tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  2:1205779/1‑93 (MQ=255)
                                                 acaTTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCaa                                        >  2:3193035/1‑93 (MQ=255)
                                                  cattcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAAc                                       <  1:484971/93‑1 (MQ=255)
                                                    ttcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     <  2:4050456/93‑1 (MQ=255)
                                                    ttcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     >  1:1488776/1‑93 (MQ=255)
                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:894315/1‑93 (MQ=255)
                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:894318/1‑93 (MQ=255)
                                                           aTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaa                              >  1:2676806/1‑93 (MQ=255)
                                                            tttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaaa                             >  2:3464026/1‑93 (MQ=255)
                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  2:1471304/93‑1 (MQ=255)
                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  1:159482/93‑1 (MQ=255)
                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  2:3211957/93‑1 (MQ=255)
                                                               gAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCt                          <  1:3271499/93‑1 (MQ=255)
                                                               gAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCt                          <  2:4726060/93‑1 (MQ=255)
                                                                aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         >  1:4693113/1‑93 (MQ=255)
                                                                aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         <  2:237773/93‑1 (MQ=255)
                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  2:3881247/93‑1 (MQ=255)
                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  1:4536662/93‑1 (MQ=255)
                                                                  tCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTa                       >  1:1165702/1‑93 (MQ=255)
                                                                   ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      >  1:3105266/1‑93 (MQ=255)
                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:2224216/1‑93 (MQ=255)
                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:1684535/1‑93 (MQ=255)
                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:2993093/1‑93 (MQ=255)
                                                                     ttCGTCATTTTATAAAGATACCGCCCCTAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGa                    >  2:3846548/1‑93 (MQ=255)
                                                                      tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   >  1:4610920/1‑93 (MQ=255)
                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:3798888/93‑1 (MQ=255)
                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:3038918/93‑1 (MQ=255)
                                                                        gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 <  2:2836986/93‑1 (MQ=255)
                                                                        gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 >  1:288187/1‑93 (MQ=255)
                                                                         tCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTACTTTTCTTACTTGCTCAACATCAATAAACCCTTTACGAAATg                >  1:3627344/1‑93 (MQ=255)
                                                                          cATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGc               <  1:2688950/93‑1 (MQ=255)
                                                                          cATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGc               >  2:3496988/1‑93 (MQ=255)
                                                                             tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            <  2:867390/93‑1 (MQ=255)
                                                                             tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            >  1:3456947/1‑93 (MQ=255)
                                                                              ttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtc           <  2:2078905/93‑1 (MQ=255)
                                                                               tataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtct          <  1:4331100/93‑1 (MQ=255)
                                                                               tataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtct          >  1:1895752/1‑93 (MQ=255)
                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         >  1:3776467/1‑93 (MQ=255)
                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         >  1:3354020/1‑93 (MQ=255)
                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:3065095/93‑1 (MQ=255)
                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:245225/93‑1 (MQ=255)
                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  1:316255/93‑1 (MQ=255)
                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        >  1:1320314/1‑93 (MQ=255)
                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  1:1206385/93‑1 (MQ=255)
                                                                                  aaaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCtt       <  1:2058850/93‑1 (MQ=255)
                                                                                  aaaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCtt       <  1:3578964/93‑1 (MQ=255)
                                                                                   aaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTc      >  1:2039603/1‑93 (MQ=255)
                                                                                    aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     <  2:2535603/93‑1 (MQ=255)
                                                                                     gATACCGCCCAGAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    >  2:4679156/1‑93 (MQ=255)
                                                                                     aaTACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    <  1:1627639/92‑1 (MQ=255)
                                                                                      aTACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagg   <  2:1829025/93‑1 (MQ=255)
                                                                                       tACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagga  <  1:3151022/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑