Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,654 T→C A232A (GCA→GCG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,6540TC99.4% 505.2 / ‑4.8 157A232A (GCA→GCG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (70/86);  total (71/86)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 160 total aligned reads displayed.

GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT  >  NC_000913/2111564‑2111742
                                                                                          |                                                                                        
 tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCATTCAGCTGAATACGTCCCCACGCa                                                                                       >  2:2619502/1‑93 (MQ=255)
 tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                                                                                       >  2:3667027/1‑93 (MQ=255)
  cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                      <  2:1362133/93‑1 (MQ=255)
   gTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcc                                                                                     >  2:1733716/1‑93 (MQ=255)
    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                    >  2:3228195/1‑93 (MQ=255)
    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                    >  2:3228194/1‑93 (MQ=255)
     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                   <  2:800278/93‑1 (MQ=255)
     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                   >  1:4117399/1‑93 (MQ=255)
       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                 >  2:1714688/1‑93 (MQ=255)
       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                 <  1:3425725/93‑1 (MQ=255)
         gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg                                                                               >  1:1572611/1‑93 (MQ=255)
          tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                              <  2:2134063/93‑1 (MQ=255)
            gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCCGGCTGAGt                                                                            >  1:4304986/1‑93 (MQ=255)
            gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt                                                                            >  1:317142/1‑93 (MQ=255)
             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                           <  1:3421316/93‑1 (MQ=255)
             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                           <  1:1654458/93‑1 (MQ=255)
                  catcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTc                                                                      <  1:1314272/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:1775123/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  1:2249166/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:662641/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:1775124/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     >  1:2980763/1‑93 (MQ=255)
                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                    >  1:1772204/1‑93 (MQ=255)
                      aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt                                                                  >  1:2702497/1‑93 (MQ=255)
                        cgttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTc                                                                <  2:3641006/91‑1 (MQ=255)
                         gttGCATCAACAGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTcc                                                               >  2:67758/2‑93 (MQ=255)
                          ttGCATCAACGGATTGTTTTGTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCg                                                              <  1:1990606/93‑1 (MQ=255)
                            gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                            <  1:963101/93‑1 (MQ=255)
                            gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                            >  2:2140314/1‑93 (MQ=255)
                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCa                                                          >  1:1105026/1‑93 (MQ=255)
                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGCTCCGGCCa                                                          >  1:1703364/1‑93 (MQ=255)
                                cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                        <  2:4267701/93‑1 (MQ=255)
                                cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                        <  1:2294609/93‑1 (MQ=255)
                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCGAGTTCCGGCCAAat                                                       >  1:678237/1‑93 (MQ=255)
                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       >  1:4337433/1‑93 (MQ=255)
                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       >  2:856422/1‑93 (MQ=255)
                                 aaCGGAATGGTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       <  2:4641547/93‑1 (MQ=255)
                                  aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAata                                                      <  2:3474858/93‑1 (MQ=255)
                                   cGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCTGCCAAatat                                                     >  2:2370085/1‑93 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  1:4748744/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  2:443063/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  2:4543333/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  1:751805/93‑1 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:2603388/1‑93 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   <  2:2819845/93‑1 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:1160370/1‑93 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:3832740/1‑93 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAAGAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   <  1:270715/93‑1 (MQ=255)
                                         gTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAg                                               <  1:1232948/93‑1 (MQ=255)
                                           tttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAgca                                             >  1:969630/1‑91 (MQ=255)
                                            ttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaaa                                            <  2:2085410/93‑1 (MQ=255)
                                                 gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                       <  1:547344/93‑1 (MQ=255)
                                                 gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                       >  2:3756588/1‑93 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                      >  1:1991396/1‑93 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  2:2752770/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  2:4254074/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  1:4627411/93‑1 (MQ=255)
                                                    aGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAg                                    >  2:559772/1‑93 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                   <  1:1637594/93‑1 (MQ=255)
                                                      cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                  >  2:4649931/1‑93 (MQ=255)
                                                      cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                  <  1:1171127/93‑1 (MQ=255)
                                                       tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                 >  1:3733109/1‑93 (MQ=255)
                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                <  1:416653/93‑1 (MQ=255)
                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                >  2:3563064/1‑93 (MQ=255)
                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGAACATAGCGAc                                >  2:937088/1‑93 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                               >  1:3383654/1‑93 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                               <  1:4441046/93‑1 (MQ=255)
                                                          gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                              <  2:2658897/93‑1 (MQ=255)
                                                          gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                              >  2:2337489/1‑93 (MQ=255)
                                                               aGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                         >  2:2326215/1‑93 (MQ=255)
                                                               aGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                         >  1:3127388/1‑93 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                        >  2:3469966/1‑93 (MQ=255)
                                                                  aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                      >  1:3055945/1‑93 (MQ=255)
                                                                  aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                      >  1:2743429/1‑93 (MQ=255)
                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                     >  1:1027610/1‑93 (MQ=255)
                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                     <  2:1659566/93‑1 (MQ=255)
                                                                    cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                    <  2:2816583/93‑1 (MQ=255)
                                                                    cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                    >  2:3256989/1‑93 (MQ=255)
                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                  >  1:469431/1‑93 (MQ=255)
                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                  <  2:337688/93‑1 (MQ=255)
                                                                       tcagCTGAATACGTCCCCACGCACCAGGCTGATTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGt                 >  2:3639559/1‑93 (MQ=255)
                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                <  2:3771853/93‑1 (MQ=255)
                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                <  1:2643393/93‑1 (MQ=255)
                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt               >  1:3610248/1‑93 (MQ=255)
                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt               <  2:1863528/93‑1 (MQ=255)
                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg              <  2:3360636/93‑1 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa             <  1:2563753/93‑1 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa             <  1:1756613/93‑1 (MQ=255)
                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  2:1081978/1‑93 (MQ=255)
                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  1:52284/1‑93 (MQ=255)
                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  2:622777/1‑93 (MQ=255)
                                                                             gAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGt           <  1:3010565/93‑1 (MQ=255)
                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         <  1:3244335/93‑1 (MQ=255)
                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         >  2:4164044/1‑93 (MQ=255)
                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         >  1:1668177/1‑93 (MQ=255)
                                                                                 aCGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAg       >  1:3889826/1‑93 (MQ=255)
                                                                                  cGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGc      <  1:1993254/93‑1 (MQ=255)
                                                                                    gccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGt    <  2:2534198/92‑1 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc   <  2:1831252/93‑1 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt  <  1:3143500/93‑1 (MQ=255)
                                                                                          |                                                                                        
GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT  >  NC_000913/2111564‑2111742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑