BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,093	A→G	C20R (TGC→CGC)	b2073 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,093	0	A	G	97.7%	408.7 / ‑2.5	133	C20R (TGC→CGC)	b2073	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/3); new base G (60/70); total (60/73)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 138 total aligned reads displayed.

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

catcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCGGCAGCAAAATGCAGGGGCGAc                                                                                         <  2:3536667/93‑1 (MQ=255)

 atcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACg                                                                                        >  2:1703386/1‑93 (MQ=255)

     cGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGt                                                                                    <  1:3827577/93‑1 (MQ=255)

      gAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGtt                                                                                   >  1:4224064/1‑93 (MQ=255)

       aaaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTc                                                                                  <  2:4134968/93‑1 (MQ=255)

       aaaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTc                                                                                  <  1:2619009/93‑1 (MQ=255)

       aaaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTc                                                                                  <  1:397366/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  2:1082992/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  1:4217740/93‑1 (MQ=255)

         aGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCg                                                                                >  1:220452/1‑93 (MQ=255)

          ggTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCgg                                                                               >  2:3785921/1‑93 (MQ=255)

           gTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCggg                                                                              >  1:232449/1‑93 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGt                                                                             <  1:2331527/93‑1 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            >  1:4648539/1‑93 (MQ=255)

              aCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTa                                                                           >  1:1363364/1‑93 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          <  1:290603/93‑1 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          <  1:679647/93‑1 (MQ=255)

                cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACt                                                                         >  2:1577164/1‑93 (MQ=255)

                cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACt                                                                         <  1:4678652/93‑1 (MQ=255)

                 aaTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTg                                                                        <  2:3557927/93‑1 (MQ=255)

                   tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGc                                                                      >  1:1527965/1‑93 (MQ=255)

                   tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGc                                                                      <  1:2628179/93‑1 (MQ=255)

                     cGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCaa                                                                    >  2:4237055/1‑93 (MQ=255)

                     cGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGGTACTGGCaa                                                                    >  1:790729/1‑93 (MQ=255)

                      ggCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCaaa                                                                   >  1:1093107/1‑93 (MQ=255)

                      ggCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCaaa                                                                   >  1:1584713/1‑93 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 <  1:2498019/93‑1 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 >  2:4353118/1‑93 (MQ=255)

                         gTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATc                                                                <  2:2685352/93‑1 (MQ=255)

                         gTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATc                                                                >  1:3990283/1‑93 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCg                                                               >  2:4392551/1‑93 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCg                                                               >  2:1813445/1‑93 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGGTACTGGCAAAATct                                                               >  1:4501211/1‑92 (MQ=255)

                           tAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGc                                                              <  2:4160040/93‑1 (MQ=255)

                            aaGTTCATTAATAGGTCTCGCGTGCATAGAACCAGAACCATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTGCTGGCCAAATCgat                                                             <  2:3875385/93‑3 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             <  1:2148674/93‑1 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             <  1:3642414/93‑1 (MQ=255)

                              gTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtgg                                                           <  1:2331048/93‑1 (MQ=255)

                               ttCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggt                                                          <  2:948535/93‑1 (MQ=255)

                                tCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtg                                                         >  1:3432795/1‑93 (MQ=255)

                                 cATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                        >  2:11831/1‑93 (MQ=255)

                                  aTTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGCCGTGGTTCCGGGTTACTGGCAAAATCGCtggtgga                                                       >  1:1231425/1‑92 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  2:931185/1‑93 (MQ=255)

                                     aaTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaaa                                                    >  2:3653253/1‑93 (MQ=255)

                                       tAGGTCTACCGTCCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACg                                                  <  1:3679530/93‑1 (MQ=255)

                                         ggTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTg                                                <  2:873817/93‑1 (MQ=255)

                                          gTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGTAAAATCGCTGGTGGCAAACGTGa                                               >  2:3392663/1‑93 (MQ=255)

                                            cTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAtt                                             <  1:4464373/93‑1 (MQ=255)

                                            cTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAtt                                             >  1:2172308/1‑93 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            >  2:843266/1‑93 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            >  1:877271/1‑93 (MQ=255)

                                                cGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                         >  1:2778489/1‑93 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        <  2:4503776/93‑1 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        >  1:2212020/1‑93 (MQ=255)

                                                  ttCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCg                                       >  1:761124/1‑93 (MQ=255)

                                                   tCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGc                                      <  1:1393626/93‑1 (MQ=255)

                                                   tCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGc                                      <  2:1048746/93‑1 (MQ=255)

                                                    cATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGCCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCt                                     <  2:1172156/93‑1 (MQ=255)

                                                      tAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCa                                   >  2:3104534/1‑93 (MQ=255)

                                                       aGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCAt                                  >  1:4687531/1‑93 (MQ=255)

                                                       aGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCAt                                  <  1:3300998/93‑1 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 <  2:3040768/93‑1 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 <  2:542461/93‑1 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 >  2:267355/1‑93 (MQ=255)

                                                         aaCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATaa                                <  2:567772/93‑1 (MQ=255)

                                                           ccAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGa                              <  2:3987477/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            <  2:4559675/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCAGCGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTAATAAGaaa                            <  1:4276940/93‑1 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           <  2:2014961/93‑1 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           >  1:2711153/1‑93 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           >  2:2993308/1‑93 (MQ=255)

                                                               aaaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATc                          <  2:3641592/93‑1 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         <  2:2466457/93‑1 (MQ=255)

                                                                 aCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCt                        <  1:1148113/93‑1 (MQ=255)

                                                                  cATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCtt                       <  2:2598391/93‑1 (MQ=255)

                                                                  cATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCtt                       >  1:3435857/1‑93 (MQ=255)

                                                                   aTCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCttt                      <  2:2814448/93‑1 (MQ=255)

                                                                   aTCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCttt                      <  1:1648470/93‑1 (MQ=255)

                                                                       agcagcAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTa                  <  1:4641292/93‑1 (MQ=255)

                                                                        gcagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTag                 <  1:4371688/93‑1 (MQ=255)

                                                                          agcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTagag               <  1:198757/93‑1 (MQ=255)

                                                                          agcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTagag               >  2:69199/1‑93 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              <  1:1719378/93‑1 (MQ=255)

                                                                             aaaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAt            >  2:1606434/1‑93 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           <  2:1618867/93‑1 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           <  1:4125344/93‑1 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           >  2:1204910/1‑93 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           <  1:1954357/93‑1 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           >  1:1302152/1‑93 (MQ=255)

                                                                               aaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTa          <  2:1453645/93‑1 (MQ=255)

                                                                               aaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTa          <  2:211242/93‑1 (MQ=255)

                                                                               aaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTa          <  2:211241/93‑1 (MQ=255)

                                                                                  gCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg       >  1:3037608/1‑93 (MQ=255)

                                                                                  gCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg       >  2:2301763/1‑93 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     <  2:1515092/93‑1 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATAGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     >  2:4361895/1‑93 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  2:2317873/1‑93 (MQ=255)

                                                                                      gggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGt   <  2:2796485/93‑1 (MQ=255)

                                                                                      gggCGACGTGGTTCCGGGTGACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGt   <  1:3556787/93‑1 (MQ=255)

                                                                                       ggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc  <  1:3943735/93‑1 (MQ=255)

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`