BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,281	G→A	G76G (GGG→GGA)	b0257 →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,281	0	G	A	99.3%	497.7 / ‑5.3	145	G76G (GGG→GGA)	b0257	putative transposase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (70/74); total (71/74)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 154 total aligned reads displayed.

TGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGC  >  NC_000913/271191‑271370

tGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACt                                                                                         >  2:4058613/1‑93 (MQ=255)

  gcGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTgg                                                                                       <  1:1430210/92‑1 (MQ=255)

  ccGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTgg                                                                                       <  1:2816853/93‑1 (MQ=255)

  ccGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTgg                                                                                       <  1:1430209/93‑1 (MQ=255)

   cGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGt                                                                                      >  2:2616952/1‑93 (MQ=255)

   cGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGt                                                                                      >  1:328907/1‑93 (MQ=255)

    ggAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTc                                                                                     >  1:365870/1‑93 (MQ=255)

       gCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGt                                                                                  <  2:1124029/93‑1 (MQ=255)

          agaagCATCGCCACAATGGCAACCCAGAGATGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGc                                                                               >  1:3270227/1‑93 (MQ=255)

          agaagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGc                                                                               >  1:879834/1‑93 (MQ=255)

           gaagCATCGCCACAATGGCAACTCAGAGAGGATACCAGATGGGGCGCTGCCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGcc                                                                              <  1:3152063/93‑1 (MQ=17)

            aagCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCca                                                                             <  2:1857220/93‑1 (MQ=255)

             agCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcag                                                                            <  1:4145207/93‑1 (MQ=255)

              gCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcagc                                                                           <  1:2532358/93‑1 (MQ=255)

                aTCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCcagcag                                                                         >  2:1551635/1‑93 (MQ=255)

                 tCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTGATGAAAGAGCTGGGACTGGTCAGTTGCCagcagc                                                                        <  2:4618774/93‑1 (MQ=255)

                  cGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGcc                                                                       <  1:1823560/93‑1 (MQ=255)

                  cGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGcc                                                                       <  2:3930548/93‑1 (MQ=255)

                  cGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGcc                                                                       <  2:4016464/93‑1 (MQ=255)

                  cGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTAGTCAGTTGCCAGCAGcc                                                                       >  2:2702265/1‑93 (MQ=255)

                    ccACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGa                                                                     >  2:2758643/1‑93 (MQ=255)

                        aaTGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCa                                                                 >  2:1299186/1‑93 (MQ=255)

                        aaTGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCa                                                                 >  1:2267318/1‑93 (MQ=255)

                         aTGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCAc                                                                >  2:68541/1‑93 (MQ=255)

                         aTGGCAACCCAGAGAGGATACCAGATGGGGCACTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCAc                                                                <  1:2644329/93‑1 (MQ=255)

                          tGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACGGGTCAGTTGCCAGCAGCCGACTCAcc                                                               <  1:1311211/93‑1 (MQ=255)

                           ggCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCg                                                              <  1:856998/93‑1 (MQ=255)

                           ggCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCg                                                              >  2:2061525/1‑93 (MQ=255)

                           ggCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCg                                                              <  1:4357419/93‑1 (MQ=255)

                               aCCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGtat                                                          >  1:3210360/1‑93 (MQ=255)

                                cccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGtata                                                         >  1:3438548/1‑93 (MQ=255)

                                 ccAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGCTGCCAGCAGCCGACCCACCGGTATaa                                                        >  1:149703/1‑93 (MQ=255)

                                   agagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGc                                                      <  2:899968/93‑1 (MQ=255)

                                    gagagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCg                                                     >  2:324401/1‑93 (MQ=255)

                                     agagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGt                                                    <  2:3387698/93‑1 (MQ=255)

                                      gagGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTAATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTg                                                   <  2:2677356/93‑1 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGc                                                 <  2:765105/93‑1 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGc                                                 >  1:338452/1‑93 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGc                                                 >  1:338453/1‑93 (MQ=255)

                                        ggATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGc                                                 >  2:415446/1‑93 (MQ=255)

                                         gATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCg                                                >  1:1925055/1‑93 (MQ=255)

                                          aTACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCgg                                               <  1:3194037/93‑1 (MQ=255)

                                           tACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGt                                              <  2:1802617/93‑1 (MQ=255)

                                            aCCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTc                                             <  2:3030729/93‑1 (MQ=255)

                                            aCCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTc                                             >  1:3898551/1‑93 (MQ=255)

                                             ccAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCa                                            >  1:1871898/1‑93 (MQ=255)

                                              cAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCATCCGACTCACCGGTATAAGCGTGGCGGTCAt                                           >  1:218325/1‑93 (MQ=255)

                                              cAGACGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCAt                                           >  2:1477601/1‑93 (MQ=255)

                                               aGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATg                                          <  2:1540553/93‑1 (MQ=255)

                                                gATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGa                                         >  2:4381788/1‑93 (MQ=255)

                                                gATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGa                                         >  1:4666193/1‑93 (MQ=255)

                                                  tGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGc                                       >  1:1150218/1‑93 (MQ=255)

                                                   ggagcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCa                                      <  2:2079785/90‑1 (MQ=255)

                                                     ggCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACg                                    <  1:832302/93‑1 (MQ=255)

                                                      gcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGt                                   <  2:2350336/93‑1 (MQ=255)

                                                      gcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGt                                   <  2:2350335/93‑1 (MQ=255)

                                                      gcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGt                                   <  2:3557391/93‑1 (MQ=255)

                                                      gcgcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGt                                   <  2:1301875/93‑1 (MQ=255)

                                                        gcTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTg                                 >  1:1273036/1‑93 (MQ=255)

                                                         cTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGc                                <  2:559705/93‑1 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                              <  2:4322781/93‑1 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                              <  1:1355515/93‑1 (MQ=255)

                                                           ggCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTa                              <  1:1313521/93‑1 (MQ=255)

                                                            gCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTAt                             <  1:2208544/93‑1 (MQ=255)

                                                            gCTTGCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTAt                             >  1:2212085/1‑93 (MQ=255)

                                                                gCTGGCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCg                         >  1:251437/1‑93 (MQ=255)

                                                                  tGGAAGACTCATGAAAGAGCTGGGACTGGTCAGGTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGGTGCTATTCCGaa                       >  1:2928140/1‑93 (MQ=18)

                                                                   ggCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAat                      >  2:2475552/1‑93 (MQ=255)

                                                                    gCAGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatc                     <  2:1682052/93‑1 (MQ=255)

                                                                    gCACACTTATGAAAGGGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatc                     <  2:1682053/93‑1 (MQ=18)

                                                                      aGACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcat                   >  1:167519/1‑93 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATTAGCACGTTGCTATCCCGAatcatc                  >  1:4696835/1‑93 (MQ=255)

                                                                       gACTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAatcatc                  <  1:3854941/93‑1 (MQ=255)

                                                                        aCTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCt                 <  1:3238831/93‑1 (MQ=255)

                                                                        aCTCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCt                 >  1:288515/1‑93 (MQ=255)

                                                                          tCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCttt               >  1:2931374/1‑92 (MQ=255)

                                                                          tCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTg               >  1:1733837/1‑93 (MQ=255)

                                                                          tCATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTg               >  1:1733919/1‑93 (MQ=255)

                                                                           cATGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGa              <  1:1149316/93‑1 (MQ=255)

                                                                            aTGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAg             >  1:2748658/1‑93 (MQ=255)

                                                                             tGAAAGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGc            <  1:2331366/93‑1 (MQ=255)

                                                                               aaaGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGa          <  2:3899011/93‑1 (MQ=255)

                                                                                aaGAGCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGAc         >  2:4388339/1‑93 (MQ=255)

                                                                                 agagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa        >  2:2946975/1‑93 (MQ=255)

                                                                                 agagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa        <  1:2159408/93‑1 (MQ=255)

                                                                                 agagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACa        >  1:3422309/1‑93 (MQ=255)

                                                                                  gagTTGGGGCTGGTCAGTTGCCAGCAGCCGAATCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAg       <  2:3501807/93‑1 (MQ=255)

                                                                                  gagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAg       >  2:278839/1‑93 (MQ=255)

                                                                                  gagCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAg       >  1:4483032/1‑93 (MQ=255)

                                                                                   agCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGt      <  2:2722038/93‑1 (MQ=255)

                                                                                   agCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGt      >  1:4528884/1‑93 (MQ=255)

                                                                                   agCTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGt      <  1:1560974/93‑1 (MQ=255)

                                                                                     cTGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTc    <  2:609308/93‑1 (MQ=255)

                                                                                      tGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTTGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCg   >  1:2124485/1‑93 (MQ=255)

                                                                                      tGGGACTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGGCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCg   >  1:2530965/1‑93 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGc  >  1:2726408/1‑93 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGc  >  2:4201464/1‑93 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGc  >  1:1634864/1‑93 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGAGACAGTTCGc  >  2:4725537/1‑93 (MQ=255)

                                                                                       gggaCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGAACGTTGCTATCCCGACTCATCTTGAGCGACAGTTCGc  >  1:1634865/1‑93 (MQ=255)

TGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGC  >  NC_000913/271191‑271370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`