BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,162,354	A→G	E19E (GAA→GAG)	baeR →	BaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,162,354	0	A	G	97.7%	411.1 / ‑1.1	131	E19E (GAA→GAG)	baeR	BaeR
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (59/69); total (60/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 134 total aligned reads displayed.

AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443

agTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGcc                                                                                         <  2:2804945/93‑1 (MQ=255)

agTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGcc                                                                                         <  1:1621180/93‑1 (MQ=255)

   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      >  2:4037326/1‑93 (MQ=255)

   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  2:3837539/93‑1 (MQ=255)

   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  2:1162342/93‑1 (MQ=255)

   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  1:2784881/93‑1 (MQ=255)

    aCCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAg                                                                                     >  2:547291/1‑93 (MQ=255)

     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:2789272/93‑1 (MQ=255)

     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:152913/93‑1 (MQ=255)

     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  1:557087/93‑1 (MQ=255)

      cGCTGGAGCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCt                                                                                   <  1:1387462/93‑1 (MQ=255)

       gCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTg                                                                                  >  2:970201/1‑93 (MQ=255)

         tGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTggg                                                                                <  1:3916486/93‑1 (MQ=255)

         tGGAACGGGATTTACAGAGAGAAGTAAGACCGAGTTACCAATAGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGCGCCGACGCTggg                                                                                <  2:4200326/93‑1 (MQ=255)

          ggAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTgggg                                                                               <  1:3300655/93‑1 (MQ=255)

           gAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGc                                                                              >  2:4487170/1‑93 (MQ=255)

            aaCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCa                                                                             <  1:2833999/93‑1 (MQ=255)

             aCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAg                                                                            >  2:1302541/1‑93 (MQ=255)

              cGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGt                                                                           <  2:1700994/93‑1 (MQ=255)

              agggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACCCACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGt                                                                           <  1:1266250/92‑1 (MQ=255)

               gggATTTTAAGAGAGAAGTAAGACCGAATTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          <  2:3199504/93‑1 (MQ=255)

               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          >  2:2469263/1‑93 (MQ=255)

               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          >  2:2728658/1‑93 (MQ=255)

               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          <  1:272781/93‑1 (MQ=255)

               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGCATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtg                                                                          >  1:2689879/1‑92 (MQ=255)

                ggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTg                                                                         >  2:351508/1‑93 (MQ=255)

                ggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTg                                                                         >  1:2249895/1‑93 (MQ=255)

                 gATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGCAGATGGGGGAGTTGc                                                                        >  2:2694221/1‑93 (MQ=255)

                    ttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCa                                                                     <  1:100157/93‑1 (MQ=255)

                    ttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCa                                                                     >  2:3223293/1‑93 (MQ=255)

                     tACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAt                                                                    <  2:408128/93‑1 (MQ=255)

                      aCAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAtt                                                                   <  2:929010/93‑1 (MQ=255)

                        agagagAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGa                                                                 <  2:2434745/93‑1 (MQ=255)

                        agagagAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGa                                                                 >  1:4593738/1‑93 (MQ=255)

                         gagagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAt                                                                <  1:1102587/93‑1 (MQ=255)

                          agagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAtt                                                               <  1:427779/93‑1 (MQ=255)

                          agagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGCGCAGTTGCTCATTGAtt                                                               >  2:4536600/1‑93 (MQ=255)

                           gagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTa                                                              >  1:1366456/1‑93 (MQ=255)

                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  1:1408749/93‑1 (MQ=255)

                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  1:4332885/93‑1 (MQ=255)

                                gTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGc                                                         <  1:1855587/93‑1 (MQ=255)

                                   tGACCGAGTTACCAATCGACGAACACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCTTTGATTATCTGCGtg                                                      >  1:1422672/1‑93 (MQ=255)

                                   tGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCggg                                                      >  1:795243/1‑91 (MQ=255)

                                   tGACCGAGTCACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtg                                                      <  2:3259424/93‑1 (MQ=255)

                                       cGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgctgc                                                  >  2:3351300/1‑93 (MQ=255)

                                        gAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCg                                                 >  1:738142/1‑93 (MQ=255)

                                          gTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAg                                               >  1:3018363/1‑93 (MQ=255)

                                           ttACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGc                                              <  1:2446873/93‑1 (MQ=255)

                                             aCCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTa                                            <  2:898058/93‑1 (MQ=255)

                                              ccAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAt                                           >  1:4441312/1‑93 (MQ=255)

                                              ccAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAt                                           >  1:1716471/1‑93 (MQ=255)

                                               cAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATg                                          <  1:1875911/93‑1 (MQ=255)

                                               cAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATg                                          >  1:2117833/1‑93 (MQ=255)

                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         <  1:437451/93‑1 (MQ=255)

                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         >  2:4504985/1‑93 (MQ=255)

                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         <  1:4512787/93‑1 (MQ=255)

                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATGGATTATCCGCGTGCTGCGAGCCAtac                                         >  2:2861892/1‑91 (MQ=255)

                                                  tCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgcgc                                       >  1:511573/1‑93 (MQ=255)

                                                  tCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgcgc                                       <  2:4045837/93‑1 (MQ=255)

                                                    gacgaAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCg                                     >  1:2522982/1‑93 (MQ=255)

                                                        aaaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGc                                 >  2:1438321/1‑93 (MQ=255)

                                                         aaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCt                                >  1:263930/1‑93 (MQ=255)

                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               <  2:514001/93‑1 (MQ=255)

                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               >  2:535883/1‑93 (MQ=255)

                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               <  1:23184/93‑1 (MQ=255)

                                                            cacacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAt                             <  2:1817848/93‑1 (MQ=255)

                                                            cacacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAt                             <  1:4678246/93‑1 (MQ=255)

                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  1:745931/93‑1 (MQ=255)

                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            >  2:2346391/1‑93 (MQ=255)

                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  1:233814/93‑1 (MQ=255)

                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  2:4655689/93‑1 (MQ=255)

                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  2:4655690/93‑1 (MQ=255)

                                                               cccGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAg                          <  2:4534078/92‑1 (MQ=255)

                                                                 cgcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGcc                        >  2:2732869/1‑93 (MQ=255)

                                                                  gcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCa                       >  2:2323536/1‑93 (MQ=255)

                                                                  gcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCa                       <  2:4555083/93‑1 (MQ=255)

                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGcccc                      >  2:3414048/1‑91 (MQ=255)

                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAc                      <  1:3191609/93‑1 (MQ=255)

                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAc                      <  1:2471199/93‑1 (MQ=255)

                                                                    gTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACg                     >  2:2978838/1‑93 (MQ=255)

                                                                    gTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACg                     >  1:2454850/1‑93 (MQ=255)

                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    <  1:4173314/93‑1 (MQ=255)

                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    <  1:3364763/93‑1 (MQ=255)

                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    >  1:2454954/1‑93 (MQ=255)

                                                                      aTTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGc                   <  1:790001/93‑1 (MQ=255)

                                                                         ttGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGAt                >  2:3482325/1‑93 (MQ=255)

                                                                          tGATGGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATc               <  1:1044732/93‑1 (MQ=255)

                                                                               gTGGAAGATGAACCGAAGCTGGCGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          >  1:4035052/1‑93 (MQ=255)

                                                                                tGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAc         >  2:887624/1‑93 (MQ=255)

                                                                                 ggAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACt        <  1:3721854/93‑1 (MQ=255)

                                                                                 ggAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACt        >  1:3664783/1‑93 (MQ=255)

                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTg       >  2:2530119/1‑93 (MQ=255)

                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTg       <  2:652501/93‑1 (MQ=255)

                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCACCCACGGCGATCAGGTACTg       >  2:3542744/1‑93 (MQ=255)

                                                                                    aGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGcc     >  1:2598201/1‑93 (MQ=255)

                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  2:617867/1‑93 (MQ=255)

                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  1:1553222/1‑93 (MQ=255)

                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  1:1430529/1‑93 (MQ=255)

                                                                                      aTGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAGTGCCGt   >  1:4052699/1‑93 (MQ=255)

                                                                                       tGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTa  <  2:1451277/93‑1 (MQ=255)

AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`