BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,917	A→G	L41P (CTG→CCG)	afuC ←	AfuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,917	0	A	G	98.7%	491.1 / ‑3.7	157	L41P (CTG→CCG)	afuC	AfuC
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (75/80); total (76/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 168 total aligned reads displayed.

CCATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCA  >  NC_000913/277827‑278006

ccATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGagcggc                                                                                         >  1:979875/1‑93 (MQ=255)

 cATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAgcggcg                                                                                        >  1:388953/1‑93 (MQ=255)

 cATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAgcggcg                                                                                        >  1:388952/1‑93 (MQ=255)

 cATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAgcggcg                                                                                        >  1:1233096/1‑93 (MQ=255)

 cATAAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCAGAgcggcg                                                                                        >  2:4646650/1‑93 (MQ=255)

  aTCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGt                                                                                       >  1:2042952/1‑93 (MQ=255)

   tCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTc                                                                                      >  1:3614356/1‑93 (MQ=255)

   tCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTc                                                                                      >  2:3325933/1‑93 (MQ=255)

   tCAATGAAAATTTGCCCTTCGCTCGGCTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTc                                                                                      >  2:4373336/1‑93 (MQ=255)

        gAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCAt                                                                                 >  2:1533616/1‑93 (MQ=255)

             tttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGcc                                                                            <  1:1198791/93‑1 (MQ=255)

                gCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCt                                                                         >  1:3197410/1‑93 (MQ=255)

                gCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCt                                                                         >  2:3500883/1‑93 (MQ=255)

                  ccTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGc                                                                       <  1:3814003/93‑1 (MQ=255)

                   cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCg                                                                      >  1:1610259/1‑93 (MQ=255)

                   cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCg                                                                      >  1:1651376/1‑93 (MQ=255)

                   cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCg                                                                      >  1:1986595/1‑93 (MQ=255)

                    ttCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGAAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCgg                                                                     >  2:3604696/1‑93 (MQ=255)

                      cGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGa                                                                   >  2:3839065/1‑93 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  >  1:2952990/1‑93 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  <  1:2579886/93‑1 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  <  1:407194/93‑1 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  >  2:1628207/1‑93 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  <  1:4553827/93‑1 (MQ=255)

                        cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATg                                                                 >  2:3143919/1‑93 (MQ=255)

                        cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATg                                                                 >  2:3836270/1‑93 (MQ=255)

                         tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATgg                                                                <  2:2877362/93‑1 (MQ=255)

                         tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATgg                                                                <  1:984088/93‑1 (MQ=255)

                         tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATgg                                                                <  2:4308036/93‑1 (MQ=255)

                         tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATgg                                                                >  2:2667098/1‑93 (MQ=255)

                          cGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCTAGCGGCGTCACCATTTGCCCCTGCGGGATGGt                                                               <  2:170682/93‑1 (MQ=255)

                          cGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGt                                                               <  1:1937271/93‑1 (MQ=255)

                          cGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGt                                                               >  1:4320027/1‑93 (MQ=255)

                          cGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGt                                                               <  2:3030867/93‑1 (MQ=255)

                           ggTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCGCCATTTGCCCCTGCGGGATGGTg                                                              <  1:4675930/93‑1 (MQ=255)

                           ggTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTg                                                              >  2:2355740/1‑93 (MQ=255)

                            gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGCTGGTGa                                                             >  1:2275367/1‑93 (MQ=255)

                            gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTgg                                                             >  1:4504116/1‑92 (MQ=255)

                            gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGa                                                             >  1:694723/1‑93 (MQ=255)

                            gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGa                                                             <  1:1667544/93‑1 (MQ=255)

                            gTTTTTCCAGCCAGGCAACCAGGCGCAAAAGAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGa                                                             <  1:13643/93‑1 (MQ=255)

                             tttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAg                                                            <  2:3043837/93‑1 (MQ=255)

                               tttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGt                                                          <  2:1817074/93‑1 (MQ=255)

                                ttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGtt                                                         <  1:4542324/93‑1 (MQ=255)

                                ttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGtt                                                         <  1:342864/93‑1 (MQ=255)

                                     gCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGTGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGatat                                                    >  1:1425988/1‑93 (MQ=255)

                                      cccGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATAtt                                                   >  2:3315004/1‑93 (MQ=255)

                                       ccGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTg                                                  <  1:4420830/93‑1 (MQ=255)

                                       ccGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTg                                                  >  1:213544/1‑93 (MQ=255)

                                       ccGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTg                                                  <  2:427947/93‑1 (MQ=255)

                                        cGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGt                                                 >  1:4741181/1‑93 (MQ=255)

                                        cGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGt                                                 <  1:681827/93‑1 (MQ=255)

                                         ggCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTc                                                <  2:2405910/93‑1 (MQ=255)

                                         ggCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTc                                                <  1:3311809/93‑1 (MQ=255)

                                         ggCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTc                                                <  1:2419304/93‑1 (MQ=255)

                                          gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCg                                               >  2:4546865/1‑93 (MQ=255)

                                            aaCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGAt                                             >  1:3356004/1‑93 (MQ=255)

                                             aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGAtt                                            <  2:320034/93‑1 (MQ=255)

                                             aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGAtt                                            <  2:815457/93‑1 (MQ=255)

                                              ccAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTa                                           <  1:1566925/93‑1 (MQ=255)

                                                aGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTAcc                                         >  1:1124802/1‑93 (MQ=255)

                                                aGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTAcc                                         <  1:48528/93‑1 (MQ=255)

                                                 ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCg                                        >  2:528362/1‑93 (MQ=255)

                                                 ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCg                                        <  1:910285/93‑1 (MQ=255)

                                                 ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCg                                        >  2:3166071/1‑93 (MQ=255)

                                                  gcgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCAGGATGGTGAGGTTGATATTGTCGATTACCGt                                       <  1:3264417/93‑1 (MQ=255)

                                                     cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTAtt                                    <  1:3277083/93‑1 (MQ=255)

                                                       aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                  >  2:3766400/1‑93 (MQ=255)

                                                       aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                  >  2:3697735/1‑93 (MQ=255)

                                                       aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                  <  1:2913345/93‑1 (MQ=255)

                                                       aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                  <  1:4472438/93‑1 (MQ=255)

                                                         aTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTg                                >  1:1003343/1‑93 (MQ=255)

                                                            gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                             <  2:1214192/93‑1 (MQ=255)

                                                               gTTTTTCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAAt                          <  1:1550046/93‑1 (MQ=255)

                                                                   tGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGtt                      >  1:933991/1‑93 (MQ=255)

                                                                    gCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGttt                     <  2:111719/93‑1 (MQ=255)

                                                                    gCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGttt                     <  1:2440078/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  1:4629733/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  1:1784051/93‑1 (MQ=255)

                                                                      cGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                   >  1:4262688/1‑93 (MQ=255)

                                                                      cGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                   <  1:3739297/93‑1 (MQ=255)

                                                                      cGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                   <  2:1868182/93‑1 (MQ=255)

                                                                       gCAGCCGGCCGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGt                  <  2:125177/93‑1 (MQ=255)

                                                                       gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGt                  >  1:3752443/1‑93 (MQ=255)

                                                                        cAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTg                 >  1:2802723/1‑93 (MQ=255)

                                                                        cAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTg                 <  1:97469/93‑1 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTTAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               <  2:673123/93‑1 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGag               >  2:684337/1‑92 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               >  1:4577207/1‑93 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               <  1:749322/93‑1 (MQ=255)

                                                                           ccGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACg              >  2:4184542/1‑93 (MQ=255)

                                                                            cGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGt             <  2:3123840/93‑1 (MQ=255)

                                                                             ggACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt            >  2:1536147/1‑93 (MQ=255)

                                                                              gACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTg           >  2:450919/1‑93 (MQ=255)

                                                                               aCGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTgc          <  1:2079351/93‑1 (MQ=255)

                                                                               aCGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTgc          >  1:1208263/1‑93 (MQ=255)

                                                                               aCGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTgc          >  1:1208264/1‑93 (MQ=255)

                                                                                    ccGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGt     >  2:1452992/1‑93 (MQ=255)

                                                                                     cGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGtt    >  1:609701/1‑93 (MQ=255)

                                                                                       agcggcGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAGGTTGCGCAGTTCa  >  1:1372838/1‑93 (MQ=255)

CCATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCA  >  NC_000913/277827‑278006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`