BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,142	T→C	R141R (CGT→CGC)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,142	0	T	C	98.7%	494.0 / ‑4.2	154	R141R (CGT→CGC)	yeiI	putative kinase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (62/90); total (63/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TTTGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

tttGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGctt                                                                                         >  2:1649856/1‑93 (MQ=255)

tttGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGctt                                                                                         >  1:18741/1‑93 (MQ=255)

  tGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCACTTGc                                                                                       <  2:1648521/93‑1 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     <  2:3831610/93‑1 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     <  1:183702/93‑1 (MQ=255)

     tttCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATg                                                                                    >  2:1600163/1‑93 (MQ=255)

     tttCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATg                                                                                    >  1:3944735/1‑93 (MQ=255)

        cAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTc                                                                                 <  1:2728436/93‑1 (MQ=255)

        cAGTGATTGGCGATGACTTTT‑ATGGCGAAAGGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTc                                                                                 <  1:2728435/93‑1 (MQ=255)

         aGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATTGTCa                                                                                >  1:1100081/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  2:446527/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  1:3459665/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  2:3054990/1‑93 (MQ=255)

          gTGATTGGC‑ATGGTTTTTGATGGCAAAATGCTCGTGGAAGAAGCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               <  2:1872859/93‑1 (MQ=255)

           tGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  2:2489989/93‑1 (MQ=255)

           ggATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  1:3908646/92‑1 (MQ=255)

            gATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAg                                                                             >  1:1561469/1‑93 (MQ=255)

               tGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGcac                                                                          <  2:4093306/93‑1 (MQ=255)

                 gCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACAt                                                                        <  2:3991842/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:2633198/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:1262720/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  1:3788710/93‑1 (MQ=255)

                   gATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCg                                                                      >  1:4258060/1‑93 (MQ=255)

                   gATGACTTTT‑ATGGCGAAATGCTACTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCg                                                                      <  2:2587584/93‑1 (MQ=255)

                    aTGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGa                                                                     >  1:261723/1‑93 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  1:653433/93‑1 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  2:61011/93‑1 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  1:3777370/93‑1 (MQ=255)

                      gACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACg                                                                   <  2:484767/93‑1 (MQ=255)

                      gACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACg                                                                   <  2:1393226/93‑1 (MQ=255)

                       aCTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGt                                                                  >  2:1712630/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAt                                                                >  2:149529/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAt                                                                >  2:796661/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGCTTCCGGCTGCGTTCGTTTGCATGGTCACAGCACATCGACGTAt                                                                >  1:738400/1‑93 (MQ=255)

                          ttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATc                                                               <  2:440373/93‑1 (MQ=255)

                           tt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCt                                                              <  1:2902166/93‑1 (MQ=255)

                            t‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTg                                                             >  1:2054382/1‑93 (MQ=255)

                                   gAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAAtt                                                       >  2:3260651/1‑93 (MQ=255)

                                   gAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAAtt                                                       >  1:3606087/1‑93 (MQ=255)

                                     aaTGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGc                                                     >  2:2129291/1‑93 (MQ=255)

                                        gCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCaa                                                  >  2:4459431/1‑93 (MQ=255)

                                        gCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCaa                                                  <  1:510821/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                <  1:1575197/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                >  1:737854/1‑93 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                <  1:2998441/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                >  2:4642351/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  1:2572567/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  2:3445580/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  2:3257478/93‑1 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  1:2159047/93‑1 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  1:4401014/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGACGGCGTGAATGGCTCCGGCTGCGTTCGCTTGCATGGTCAGAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  1:1646268/93‑1 (MQ=255)

                                             tGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgag                                             <  2:3565749/93‑1 (MQ=255)

                                             tGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgag                                             <  2:2506909/93‑1 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  2:429227/93‑1 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           >  2:2673174/1‑93 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  1:4112858/93‑1 (MQ=255)

                                                 agaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACgc                                         >  1:3083261/1‑92 (MQ=255)

                                                 agaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGa                                         <  1:2344791/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  1:500045/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  2:454968/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCGGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  1:4572417/93‑1 (MQ=255)

                                                   aaaCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATc                                       <  1:3818821/93‑1 (MQ=255)

                                                      cgcgCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAACCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaaa                                    <  2:3111621/93‑1 (MQ=255)

                                                       gcgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAc                                   <  2:3065592/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  2:612681/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  1:2383620/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  1:1705438/93‑1 (MQ=255)

                                                         gcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCg                                 >  2:2709756/1‑93 (MQ=255)

                                                           cGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTg                               >  1:1053818/1‑93 (MQ=255)

                                                            gTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGc                              <  2:2269597/93‑1 (MQ=255)

                                                             tGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCt                             >  2:3529375/1‑93 (MQ=255)

                                                              gCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTg                            <  2:3502831/93‑1 (MQ=255)

                                                               ccGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTgg                           <  1:2375050/93‑1 (MQ=255)

                                                                cGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGc                          <  1:2677221/93‑1 (MQ=255)

                                                                cGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGc                          >  2:4269799/1‑93 (MQ=255)

                                                                    gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  1:2102467/1‑93 (MQ=255)

                                                                    gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  2:215263/1‑93 (MQ=255)

                                                                     tGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTa                     >  1:4491333/1‑93 (MQ=255)

                                                                        aTGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACg                  >  2:3832610/1‑93 (MQ=255)

                                                                         tGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGa                 <  1:3520205/93‑1 (MQ=255)

                                                                         tGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGa                 <  1:1722893/93‑1 (MQ=255)

                                                                            ctcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAc              <  1:788680/93‑1 (MQ=255)

                                                                            ctcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAc              <  2:3448040/93‑1 (MQ=255)

                                                                             tcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAcc             >  2:1253842/1‑93 (MQ=255)

                                                                             tcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAcc             >  1:3454591/1‑93 (MQ=255)

                                                                              ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  2:1995540/93‑1 (MQ=255)

                                                                              ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  1:2953895/93‑1 (MQ=255)

                                                                               cGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa           <  2:529725/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  2:1563331/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  1:2760235/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  1:3976318/93‑1 (MQ=255)

                                                                                  cTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATct        <  2:950298/93‑1 (MQ=255)

                                                                                  cTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATct        <  2:929465/93‑1 (MQ=255)

                                                                                     cgttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgct     >  1:1304821/1‑93 (MQ=255)

                                                                                      gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    >  1:4238156/1‑93 (MQ=255)

                                                                                      gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    >  2:2094081/1‑93 (MQ=255)

                                                                                       ttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTgg   >  1:253580/1‑93 (MQ=255)

                                                                                       ttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTgg   >  1:539671/1‑93 (MQ=255)

                                                                                        tcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGa  >  1:4088467/1‑93 (MQ=255)

TTTGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`