Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,317 T→C T347A (ACG→GCG)  yeiM ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3170TC98.1% 490.4 / ‑2.8 157T347A (ACG→GCG) yeiMputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (70/84);  total (72/85)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 162 total aligned reads displayed.

AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA  >  NC_000913/2254227‑2254405
                                                                                          |                                                                                        
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                        >  1:4323992/1‑93 (MQ=255)
 aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgcc                                                                                       <  1:1865161/93‑1 (MQ=255)
    tGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgccgcc                                                                                    <  2:762400/93‑1 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                               >  1:510632/1‑93 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                               >  1:2184986/1‑93 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTg                                                                              <  1:364984/93‑1 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTg                                                                              >  2:4614864/1‑93 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGc                                                                             >  1:3132258/1‑93 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGc                                                                             >  1:2794148/1‑93 (MQ=255)
            cGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCa                                                                            <  1:4003297/93‑1 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                          >  2:4481743/1‑93 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                          <  1:4640324/93‑1 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                        >  2:1282698/1‑93 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                        >  2:3880158/1‑93 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:4284540/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:2490595/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:2762382/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGAGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       >  2:1091091/1‑93 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                      <  2:2440540/93‑1 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                      <  2:2802562/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  2:1942184/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  2:802273/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  1:1084873/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggg                                                                    <  1:2820856/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggg                                                                    <  1:4348885/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGCTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATggg                                                                    >  1:4274798/1‑93 (MQ=255)
                     cGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgggg                                                                   <  2:2731037/93‑1 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggggg                                                                  >  2:3490539/1‑92 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                  >  2:3220215/1‑93 (MQ=255)
                       aTAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgag                                                                 >  1:3525573/1‑93 (MQ=255)
                       aTAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGGTTGCAGGTATGGGgag                                                                 >  1:4222878/1‑93 (MQ=255)
                        tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                >  2:4540003/1‑93 (MQ=255)
                        tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                >  2:1716669/1‑93 (MQ=255)
                         aGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaa                                                               >  1:4543411/1‑93 (MQ=255)
                          gAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaaa                                                              <  1:1249645/93‑1 (MQ=255)
                            aCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACt                                                            >  1:2912409/1‑93 (MQ=255)
                             ccAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTc                                                           <  1:3855502/93‑1 (MQ=255)
                             ccAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTc                                                           <  1:763936/93‑1 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                          <  1:1748492/93‑1 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                          >  1:905515/1‑93 (MQ=255)
                               aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                         <  2:2026877/93‑1 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCTTTTGCAGGTATGGGGAGAAACTCAgg                                                        >  1:2382603/1‑93 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                        >  1:3058407/1‑93 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                      <  1:1305206/93‑1 (MQ=255)
                                  gTTAGAAAAACCACAAAGCGCAAAGGAGATAAGCGCAATGGTTTTCACTTGCAGCGCGCCGCCCGTTTGCAGGTATGGTGAGATACTCAggga                                                      >  2:1644653/1‑91 (MQ=255)
                                   gtAGCAAAGCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                     <  1:791184/92‑1 (MQ=255)
                                    tAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAg                                                    <  1:3389519/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                 <  2:73477/93‑1 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGGGTGCCGCCCGTTTGCAGGAATGGGGGGAAACTCAGGAAAGCGACGaa                                             >  1:4495790/1‑93 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                             >  1:1587881/1‑93 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                             <  1:31074/93‑1 (MQ=255)
                                            ccACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAt                                            >  1:1060976/1‑93 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                          <  1:3239670/93‑1 (MQ=255)
                                               caAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCg                                         <  2:3799962/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        >  1:64088/1‑93 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        <  1:3967395/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        <  1:4045439/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCAGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        >  2:695914/1‑93 (MQ=255)
                                                 aaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGtt                                       <  1:959576/93‑1 (MQ=255)
                                                  aGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTa                                      <  2:314669/93‑1 (MQ=255)
                                                    cgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAt                                    <  1:2908915/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  2:580168/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   >  2:4597266/1‑93 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  1:1129018/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  2:2261400/93‑1 (MQ=255)
                                                       aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                 >  2:1451251/1‑93 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                <  1:1533856/93‑1 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                >  2:2486397/1‑93 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  1:3745264/93‑1 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               >  2:369408/1‑93 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  2:3246020/93‑1 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  2:3149916/93‑1 (MQ=255)
                                                           gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGt                             <  2:1370755/93‑1 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                           >  1:4135444/1‑93 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                           >  1:3269933/1‑93 (MQ=255)
                                                                aaTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTg                        <  1:2335353/93‑1 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                       >  1:561911/1‑93 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                       >  2:2020886/1‑93 (MQ=255)
                                                                  tCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTTGTTAATCGCCAGTTTCTGcc                      <  2:3740544/93‑1 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTNGTTAATCGCCAGTTTCTGCCCaa                   >  1:2466014/1‑93 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                   <  2:858210/93‑1 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                   >  2:1521030/1‑93 (MQ=255)
                                                                      aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                  <  2:2415158/93‑1 (MQ=255)
                                                                         ttttttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:3382205/91‑1 (MQ=255)
                                                                         ggTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:4523379/93‑1 (MQ=255)
                                                                         ggTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:1263473/93‑1 (MQ=255)
                                                                          gTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg              >  2:4422231/1‑93 (MQ=255)
                                                                           ttctCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGc             <  2:217523/90‑1 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            >  2:3518050/1‑93 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:4041466/93‑1 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:447742/93‑1 (MQ=255)
                                                                            tttCACTGCCAGCCCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:2267840/93‑1 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        >  2:1277030/1‑93 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        <  1:1054336/93‑1 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        >  2:3028062/1‑93 (MQ=255)
                                                                                 cTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCg       >  2:1651505/1‑93 (MQ=255)
                                                                                  ttCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc      >  1:1918832/1‑93 (MQ=255)
                                                                                  ttCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc      <  2:1780173/93‑1 (MQ=255)
                                                                                    ccAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa    <  2:3385559/93‑1 (MQ=255)
                                                                                      aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa  <  1:1803603/93‑1 (MQ=255)
                                                                                          |                                                                                        
AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA  >  NC_000913/2254227‑2254405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑