BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,802	T→C	F180L (TTT→CTT)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,802	0	T	C	97.4%	474.9 / ‑0.8	154	F180L (TTT→CTT)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (87/63); total (90/64)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 161 total aligned reads displayed.

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

cACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGctt                                                                                         >  1:4336094/1‑93 (MQ=255)

  cGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAc                                                                                       >  2:2223033/1‑93 (MQ=255)

  cGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAc                                                                                       <  2:2615476/93‑1 (MQ=255)

   gATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAcc                                                                                      <  1:75749/93‑1 (MQ=255)

    aTCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCg                                                                                     >  1:2598454/1‑93 (MQ=255)

     tCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGt                                                                                    >  1:3711571/1‑93 (MQ=255)

      cTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAAAGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTg                                                                                   >  1:1442790/1‑93 (MQ=255)

          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  1:225456/1‑93 (MQ=255)

          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  1:2948077/1‑93 (MQ=255)

           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              >  2:974213/1‑93 (MQ=255)

           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              >  2:467757/1‑93 (MQ=255)

            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:2210053/93‑1 (MQ=255)

            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:1613957/93‑1 (MQ=255)

             ccccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCt                                                                            <  1:1674523/93‑1 (MQ=255)

             ccccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCt                                                                            >  2:2689410/1‑93 (MQ=255)

              cccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCTAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTg                                                                           >  2:4596531/1‑93 (MQ=255)

              cccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTg                                                                           >  1:2723187/1‑93 (MQ=255)

               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          >  1:4374934/1‑93 (MQ=255)

               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          >  1:1286546/1‑93 (MQ=255)

               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTAAACCGCTTACCGTGCTCTCCTGc                                                                          <  1:834694/93‑1 (MQ=255)

                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  2:1677261/1‑93 (MQ=255)

                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  1:936264/1‑93 (MQ=255)

                  gaagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAc                                                                       <  2:1040871/93‑1 (MQ=255)

                   aagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACa                                                                      >  1:4513498/1‑93 (MQ=255)

                    agacCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     <  1:3479850/93‑1 (MQ=255)

                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     >  2:2137800/1‑93 (MQ=255)

                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     >  2:55911/1‑93 (MQ=255)

                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGAGGCCTTACACCGCTTAAATTGCTCTGCTGTGAGaa                                                                     >  2:1200161/1‑93 (MQ=255)

                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:1123955/1‑93 (MQ=255)

                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:3370436/1‑93 (MQ=255)

                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   <  1:3567370/93‑1 (MQ=255)

                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   >  2:3881018/1‑93 (MQ=255)

                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  >  1:487841/1‑93 (MQ=255)

                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  <  1:143459/93‑1 (MQ=255)

                       aCCTCACTCCGCACCGGATATTCTCGTCGAAGAGCTGAAGCGCCGCCGTGAGCACGGCCTTACACCGCTTCCCGTGCTCTCCTGCGACAatat                                                                  <  2:2867087/93‑1 (MQ=255)

                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 >  2:2469734/1‑93 (MQ=255)

                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               <  1:3751897/93‑1 (MQ=255)

                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              >  2:2934871/1‑93 (MQ=255)

                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              <  2:4629977/93‑1 (MQ=255)

                            aCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             >  1:1205682/1‑93 (MQ=255)

                            aCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             >  1:704249/1‑93 (MQ=255)

                             cTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGa                                                            <  2:2083414/93‑1 (MQ=255)

                             cTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGa                                                            <  2:853615/93‑1 (MQ=255)

                              tCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTCCGACAATATTCCCgcc                                                           >  1:1166486/1‑91 (MQ=255)

                               ccGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGGGAGCGCGGCCTTACACCGCTTACCGTGCTGTCCTGCGACAATATTCCCcaca                                                          >  2:1303498/1‑93 (MQ=255)

                                  cACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATg                                                       >  1:3968519/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      <  2:3575073/93‑1 (MQ=255)

                                      gggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   >  2:2759417/1‑93 (MQ=255)

                                       gggaTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTTTCCTGCGACAATATTCCCGACAATGGTCAt                                                  <  2:3623073/90‑1 (MQ=255)

                                         tATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgt                                                <  2:1875406/93‑1 (MQ=255)

                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  2:1454514/93‑1 (MQ=255)

                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  2:1093329/93‑1 (MQ=255)

                                            tctcTTCGAAGCACTGAAACTCCGCCGTGACCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATTGTCATgtggt                                             <  2:2964705/93‑1 (MQ=255)

                                             ctcGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATgtggtg                                            <  1:1830546/93‑1 (MQ=255)

                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGa                                           <  2:2927222/93‑1 (MQ=255)

                                               cgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaa                                          >  1:189831/1‑93 (MQ=255)

                                               cgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaa                                          <  1:2076098/93‑1 (MQ=255)

                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGacaa                                        >  1:132056/1‑93 (MQ=255)

                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        >  1:3885300/1‑93 (MQ=255)

                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  2:1537260/93‑1 (MQ=255)

                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  1:3262623/93‑1 (MQ=255)

                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  1:3556685/93‑1 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:4272257/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:2688163/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:4099638/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      <  2:1739355/93‑1 (MQ=255)

                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgc                                    >  1:3286467/1‑93 (MQ=255)

                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGGAAAAcgc                                    >  2:1716673/1‑93 (MQ=255)

                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   <  2:1985907/93‑1 (MQ=255)

                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   >  1:22980/1‑93 (MQ=255)

                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  1:2473327/1‑93 (MQ=255)

                                                         cTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTg                                >  1:3693811/1‑93 (MQ=255)

                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               >  2:350405/1‑93 (MQ=255)

                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           >  1:2485402/1‑93 (MQ=255)

                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           <  2:1212776/93‑1 (MQ=255)

                                                               cgccgcCGTGAGCGCGGCCTCACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGa                          <  2:784030/93‑1 (MQ=255)

                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  1:4523487/93‑1 (MQ=255)

                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  2:3648055/93‑1 (MQ=255)

                                                                  cgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATg                       >  1:4191008/1‑93 (MQ=255)

                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACTATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  2:3061029/1‑93 (MQ=255)

                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  1:4074380/1‑93 (MQ=255)

                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  1:49410/1‑93 (MQ=255)

                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      <  2:4381699/93‑1 (MQ=255)

                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGGTCTGGGAATgg                      >  2:131092/1‑93 (MQ=255)

                                                                     cgTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    >  1:1592068/1‑93 (MQ=255)

                                                                     cgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    >  1:1781926/1‑93 (MQ=255)

                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  1:3144683/1‑93 (MQ=255)

                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  2:3873134/1‑93 (MQ=255)

                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  2:1402979/1‑93 (MQ=255)

                                                                        gAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGCGCTGGGAATGGCAGaa                 >  1:101620/1‑93 (MQ=255)

                                                                              ggCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGt           >  1:1046471/1‑93 (MQ=255)

                                                                              ggCCTTACACCGCTTACCGTCCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGt           <  2:239665/93‑1 (MQ=255)

                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:4460576/93‑1 (MQ=255)

                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:420107/93‑1 (MQ=255)

                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:2702596/93‑1 (MQ=255)

                                                                                 cTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCg        >  2:4180226/1‑93 (MQ=255)

                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  2:4419891/1‑93 (MQ=255)

                                                                                      acCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGa   >  2:4412802/1‑93 (MQ=255)

                                                                                       ccGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGaa  <  2:3377097/93‑1 (MQ=255)

                                                                                       ccGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGaa  >  1:4471137/1‑93 (MQ=255)

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`