BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,775	A→G	Y386C (TAC→TGC)	yagG →	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,775	0	A	G	98.2%	330.7 / ‑3.3	111	Y386C (TAC→TGC)	yagG	putative permease
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (48/60); total (50/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 123 total aligned reads displayed.

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  1:3835616/1‑93 (MQ=255)

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  1:4290105/1‑93 (MQ=255)

  cTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTc                                                                                       <  2:3506239/93‑1 (MQ=255)

   tCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCa                                                                                      >  1:147201/1‑93 (MQ=255)

     gACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCCAc                                                                                    >  1:2238211/1‑93 (MQ=255)

     gACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAAc                                                                                    <  1:128300/93‑1 (MQ=255)

     gACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAAc                                                                                    <  1:110083/93‑1 (MQ=255)

     gACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAAc                                                                                    <  2:3767358/93‑1 (MQ=255)

      aCGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACt                                                                                   <  1:1398495/93‑1 (MQ=255)

      aCGGCCTGTTTTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACt                                                                                   <  1:1273889/93‑1 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  >  1:4603369/1‑93 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  <  2:1667123/93‑1 (MQ=255)

        gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 >  1:1033723/1‑93 (MQ=255)

        gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 <  1:1051376/93‑1 (MQ=255)

        gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 >  2:1192767/1‑93 (MQ=255)

        gggCTGGTGTCCTCCCCCTACCCGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 <  1:859215/93‑1 (MQ=255)

         ggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAtt                                                                                <  1:1336126/93‑1 (MQ=255)

          gCTGGTGTTCTCCCCCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                               <  1:2911314/93‑1 (MQ=255)

          gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                               <  2:3960471/93‑1 (MQ=255)

           cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                              >  2:4260219/1‑93 (MQ=255)

               tgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCca                                                                          <  2:3894344/93‑1 (MQ=255)

               tgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCca                                                                          <  1:760376/93‑1 (MQ=255)

               tgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCca                                                                          <  1:2040627/93‑1 (MQ=255)

                  tctcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagca                                                                       >  2:2759692/1‑93 (MQ=255)

                  tctcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagca                                                                       >  1:1108446/1‑93 (MQ=255)

                   ctcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCTGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcag                                                                      >  1:3064867/1‑93 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     <  2:180728/93‑1 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  2:2509053/1‑93 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  2:3080991/1‑93 (MQ=255)

                     cctcctacctGTTCAGCCTGAAGATTGTCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCGGGCGTGCGTCAACTATTCCGCcagcagca                                                                    <  2:88297/90‑1 (MQ=255)

                       ccctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCagcagcagc                                                                  <  1:3001753/92‑1 (MQ=255)

                         ctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGt                                                                <  2:2790296/93‑1 (MQ=255)

                          tacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCAAGCAGCAGCGTg                                                               >  2:3126980/1‑93 (MQ=255)

                           tcctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGc                                                              <  2:1424330/92‑1 (MQ=255)

                           acctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGc                                                              >  2:960421/1‑93 (MQ=255)

                           acctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGc                                                              <  1:1697813/93‑1 (MQ=255)

                             ctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAg                                                            <  1:971643/93‑1 (MQ=255)

                              tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                           <  1:1223806/93‑1 (MQ=255)

                              tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGCCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                           <  1:2389889/93‑1 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  1:874494/1‑93 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          <  2:664589/93‑1 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  2:801525/1‑93 (MQ=255)

                                  cAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGt                                                       >  1:3204586/1‑93 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      >  1:500962/1‑93 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGAGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      <  1:2503797/93‑1 (MQ=255)

                                    gCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTg                                                     >  1:3571231/1‑93 (MQ=255)

                                    gCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCAAGCAGCAGCGTGCAGCCGGTTg                                                     <  1:834944/93‑1 (MQ=255)

                                     ccTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGa                                                    <  2:2328436/93‑1 (MQ=255)

                                     ccTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGa                                                    <  1:3421911/93‑1 (MQ=255)

                                      ccgAAGATTGGTCCGGCGATAGGCGCGGGGGGGGTGGGCTGCGTCCTGGCGTACGTCAACTATTCCGCCAGGAGCAGCGGGCAGCCGGTTGAg                                                   <  2:3299160/91‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  <  2:465506/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  <  1:4172520/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGCCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  >  2:2853972/1‑93 (MQ=255)

                                         aaGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTg                                                >  1:4732153/1‑93 (MQ=255)

                                          aGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGc                                               >  1:4263574/1‑93 (MQ=255)

                                           gATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCt                                              >  2:3701650/1‑93 (MQ=255)

                                           gATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCt                                              >  2:1949368/1‑93 (MQ=255)

                                           gATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGGGCt                                              >  2:1444190/1‑93 (MQ=255)

                                            aTTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTc                                             >  1:693356/1‑93 (MQ=255)

                                            aTTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTc                                             >  1:3438158/1‑93 (MQ=255)

                                              tGGCCTGGCGATTGGCGGGGCGGTGGTTGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcac                                           <  1:4556208/93‑1 (MQ=255)

                                                   tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                      <  1:2551932/93‑1 (MQ=255)

                                                         ttGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGCTGCTCACCACCATCaaaa                                >  1:4013980/1‑93 (MQ=255)

                                                           ggcggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCACAAtt                              >  1:3263995/1‑93 (MQ=255)

                                                            gcggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttc                             >  2:1253855/1‑93 (MQ=255)

                                                             cggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttct                            <  2:3888906/93‑1 (MQ=255)

                                                               gggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgt                          <  1:3172631/93‑1 (MQ=255)

                                                               gggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgt                          >  2:2919529/1‑93 (MQ=255)

                                                                ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                         <  2:1627755/93‑1 (MQ=255)

                                                                ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                         <  2:2250780/93‑1 (MQ=255)

                                                                ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                         <  1:54724/93‑1 (MQ=255)

                                                                  cggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttct                       <  1:1442618/93‑1 (MQ=255)

                                                                  cggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttct                       <  1:1002127/93‑1 (MQ=255)

                                                                    gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     >  1:2091892/1‑93 (MQ=255)

                                                                    gtggtgGGCTGGATCCGGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     <  2:4714899/93‑1 (MQ=255)

                                                                     tggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCg                    >  2:578817/1‑93 (MQ=255)

                                                                      tgtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                   <  2:3493283/92‑1 (MQ=255)

                                                                      tgtggCCTGGATCCTGGCGTGCGTCAACTATTCCGCCACCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                   <  2:2351867/92‑1 (MQ=255)

                                                                      ggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                   <  1:3318931/93‑1 (MQ=255)

                                                                      ggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                   <  1:997744/93‑1 (MQ=255)

                                                                      ggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACAATCAAAATTCTGTTCTGCgt                   <  1:4730297/93‑1 (MQ=255)

                                                                        tggGTTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCCCCTCCATCAAAATTCTGTTCTGCgtgg                 <  2:1327474/93‑1 (MQ=255)

                                                                           gCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGc              >  2:2048518/1‑93 (MQ=255)

                                                                            cTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGcc             >  2:1903743/1‑93 (MQ=255)

                                                                            cTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGcc             <  1:956530/93‑1 (MQ=255)

                                                                             tGGATCCTGGCGTCCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg            >  1:3095024/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           <  1:2554964/93‑1 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           <  1:4595112/93‑1 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           >  2:1745599/1‑93 (MQ=255)

                                                                               gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt          <  1:971191/93‑1 (MQ=255)

                                                                               gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt          >  2:952352/1‑93 (MQ=255)

                                                                               gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGAAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt          >  2:3020654/1‑93 (MQ=255)

                                                                                aTCCTGGCGTGCGCGAACTGTCCCGCCAGCAGCAGCGTGCGGCCGCTTGAGGTCCTCACCGCCATCAAAATTCTGTTCAGCGTGGTGCCggtg         <  2:4044159/93‑1 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        <  1:1608873/93‑1 (MQ=255)

                                                                                  ccTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtggt       <  1:1489808/93‑1 (MQ=255)

                                                                                   cTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGgtggtg      <  2:295234/93‑1 (MQ=255)

                                                                                    tGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGc     >  1:377887/1‑93 (MQ=255)

                                                                                    tGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCT‑‑‑CACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctct  >  2:1231290/1‑93 (MQ=255)

                                                                                      gcgtgcgtCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctc   <  1:4466804/93‑1 (MQ=255)

                                                                                       cGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctct  <  1:3027846/93‑1 (MQ=255)

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`