BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,515	A→G	R151R (CGT→CGC)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,515	0	A	G	99.4%	516.3 / ‑4.7	160	R151R (CGT→CGC)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (62/96); total (64/96)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 168 total aligned reads displayed.

GAAATCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATACGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCT  >  NC_000913/2281425‑2281604

gAAATCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGcg                                                                                         >  2:4639862/1‑93 (MQ=255)

 aaaTCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATgcgc                                                                                        <  2:3362714/93‑1 (MQ=255)

  aaTCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGcgcg                                                                                       >  2:95615/1‑93 (MQ=255)

   aTCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATgcgcgc                                                                                      <  1:815375/93‑1 (MQ=255)

   aTCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATgcgcgc                                                                                      <  1:815376/93‑1 (MQ=255)

       gCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGcaaca                                                                                  >  1:392729/1‑93 (MQ=255)

        ccACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCAGTTAAATCGATGCGCGcaacaa                                                                                 >  2:4465840/1‑93 (MQ=255)

          aCTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAata                                                                               <  2:1754938/93‑1 (MQ=255)

          aCTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAata                                                                               >  1:3865805/1‑93 (MQ=255)

          aCTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAata                                                                               <  1:3442879/93‑1 (MQ=255)

           cTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAatat                                                                              >  2:4562130/1‑93 (MQ=255)

            tttGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATc                                                                             <  2:186128/93‑1 (MQ=255)

             ttGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATcc                                                                            <  2:678900/93‑1 (MQ=255)

             ttGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATcc                                                                            <  1:212047/93‑1 (MQ=255)

                cgcCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCa                                                                         <  1:1062815/93‑1 (MQ=255)

                 gcCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAt                                                                        <  2:3038242/93‑1 (MQ=255)

                  cccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtg                                                                       >  2:1816901/1‑93 (MQ=255)

                  cccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtg                                                                       >  2:3068372/1‑93 (MQ=255)

                  cccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtg                                                                       >  1:2361974/1‑93 (MQ=255)

                   ccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtga                                                                      >  2:2101904/1‑93 (MQ=255)

                   ccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtga                                                                      <  2:2618945/93‑1 (MQ=255)

                   ccTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtga                                                                      <  1:175874/93‑1 (MQ=255)

                    cTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgat                                                                     >  1:66307/1‑93 (MQ=255)

                    cTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgat                                                                     >  1:4650691/1‑93 (MQ=255)

                    cTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgat                                                                     >  1:3569009/1‑93 (MQ=255)

                     tACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgatt                                                                    >  2:693633/1‑93 (MQ=255)

                     tACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgatt                                                                    >  1:1637301/1‑93 (MQ=255)

                     tACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgatt                                                                    >  1:2798881/1‑93 (MQ=255)

                      aCTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgattg                                                                   <  2:2388812/93‑1 (MQ=255)

                       cTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgattga                                                                  <  1:2565854/93‑1 (MQ=255)

                       cTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgattga                                                                  >  2:3730508/1‑93 (MQ=255)

                        tCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCAtgattgat                                                                 >  2:2924337/1‑93 (MQ=255)

                           cccTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATc                                                              >  2:376174/1‑93 (MQ=255)

                           cccTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATc                                                              <  1:3153203/93‑1 (MQ=255)

                           cccTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGACTGATATc                                                              >  1:4340664/1‑93 (MQ=255)

                             cTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCaa                                                            >  2:2606292/1‑93 (MQ=255)

                             cTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCaa                                                            <  1:2032695/93‑1 (MQ=255)

                             cTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCaa                                                            <  1:1867812/93‑1 (MQ=255)

                             cTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCaa                                                            <  1:277771/93‑1 (MQ=255)

                              tttCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAg                                                           <  2:422846/93‑1 (MQ=255)

                              tttCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAg                                                           >  1:4372005/1‑93 (MQ=255)

                                tCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGAt                                                         >  1:4731316/1‑93 (MQ=255)

                                  cggAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATaa                                                       <  2:412994/92‑1 (MQ=255)

                                  aGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATaa                                                       <  2:412993/93‑1 (MQ=255)

                                  aGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATaa                                                       <  1:2656303/93‑1 (MQ=255)

                                   ggAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAt                                                      <  2:229728/93‑1 (MQ=255)

                                   ggAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAt                                                      >  1:329362/1‑93 (MQ=255)

                                   ggAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAt                                                      <  2:3600413/93‑1 (MQ=255)

                                    gAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtg                                                     <  2:1239538/93‑1 (MQ=255)

                                    gAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtg                                                     <  1:60564/93‑1 (MQ=255)

                                     aaaGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgg                                                    <  1:1147183/93‑1 (MQ=255)

                                      aaGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtggg                                                   <  1:3971559/93‑1 (MQ=255)

                                       aGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggt                                                  >  2:2315391/1‑93 (MQ=255)

                                       aGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggt                                                  <  2:2210625/93‑1 (MQ=255)

                                        gTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggtt                                                 <  1:910776/93‑1 (MQ=255)

                                        gTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggtt                                                 >  1:3061923/1‑93 (MQ=255)

                                         tGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggttg                                                <  2:221807/93‑1 (MQ=255)

                                          gagaTAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggttgg                                               <  1:3622020/93‑1 (MQ=255)

                                          gagaTAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggttgg                                               <  1:1937672/93‑1 (MQ=255)

                                           agaTAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggttggg                                              <  1:1663619/93‑1 (MQ=255)

                                            gaTAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAAtgggttgggt                                             <  2:993364/93‑1 (MQ=255)

                                              tAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGggttgggttg                                           >  2:2329766/1‑93 (MQ=255)

                                              tAGCGGGTGGACTCTGGATTGGTTTCCAATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGggttgggttg                                           <  2:2706320/93‑1 (MQ=255)

                                                gCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGAt                                         <  1:2400587/93‑1 (MQ=255)

                                                gCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGAt                                         <  1:2478307/93‑1 (MQ=255)

                                                acGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGAt                                         <  2:2945191/92‑1 (MQ=255)

                                                acGGGTGGACTCTGGATTGGTTTCCCATTCGGGTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGAt                                         <  1:379245/92‑1 (MQ=255)

                                                   ggTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTc                                      <  2:680085/93‑1 (MQ=255)

                                                   ggTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTc                                      <  2:3479749/93‑1 (MQ=255)

                                                     tGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCga                                    <  2:1113091/93‑1 (MQ=255)

                                                         ctctGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGAtt                                <  2:2529434/93‑1 (MQ=255)

                                                         ctctGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGAtt                                <  1:2836961/93‑1 (MQ=255)

                                                            ttgATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTc                             <  2:3842558/91‑1 (MQ=255)

                                                            tGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTc                             <  1:2445480/93‑1 (MQ=255)

                                                            tGGATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTc                             <  2:3461629/93‑1 (MQ=255)

                                                            tGGATTGGTTTCCCATTCGGTTAAATCGATACGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTc                             >  2:2140896/1‑93 (MQ=255)

                                                              gATTGGTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGt                           <  2:1111778/93‑1 (MQ=255)

                                                                 ttgTTTCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTaa                        <  1:3787256/91‑1 (MQ=255)

                                                                    tttCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAAcgc                     <  2:2439065/93‑1 (MQ=255)

                                                                     ttCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAAcgcg                    <  1:3687350/93‑1 (MQ=255)

                                                                      tCCCATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACgcgc                   >  1:2251860/1‑93 (MQ=255)

                                                                       cccATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCa                  <  1:4511311/93‑1 (MQ=255)

                                                                       cccATTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCa                  <  1:546800/93‑1 (MQ=255)

                                                                          aTTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATAc               >  2:819341/1‑93 (MQ=255)

                                                                          aTTCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATAc               >  2:819340/1‑93 (MQ=255)

                                                                           ttCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACt              >  1:466258/1‑93 (MQ=255)

                                                                           ttCGGTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACt              <  1:2541641/93‑1 (MQ=255)

                                                                               gTTAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGCTGATGTCGAGATTTTCGTTAACGCGCATACTGCTc          >  2:2063494/1‑93 (MQ=255)

                                                                                gtAAATCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCa         <  1:1561074/92‑1 (MQ=255)

                                                                                  aaaTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAgg       >  2:3426946/1‑93 (MQ=255)

                                                                                  aaaTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAgg       <  1:3384645/93‑1 (MQ=255)

                                                                                   aaTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGt      <  1:1884221/93‑1 (MQ=255)

                                                                                   aaTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGt      <  2:2350628/93‑1 (MQ=255)

                                                                                   aaTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGt      <  1:554167/93‑1 (MQ=255)

                                                                                    aTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGtt     >  1:1327800/1‑93 (MQ=255)

                                                                                    aTCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGtt     >  2:2789352/1‑93 (MQ=255)

                                                                                     tCGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTg    >  1:1494078/1‑93 (MQ=255)

                                                                                      cGATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGc   <  1:3028428/93‑1 (MQ=255)

                                                                                       gATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCt  >  1:4567579/1‑93 (MQ=255)

                                                                                       gATGCGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCt  >  1:4489570/1‑93 (MQ=255)

GAAATCCGCCACTTTGCGCCCTACTCGCCCTTTCAGGAAAGTGAGATAGCGGGTGGACTCTGGATTGGTTTCCCATTCGGTTAAATCGATACGCGCAACAATATCCGCATGATTGATATCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCT  >  NC_000913/2281425‑2281604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`